(Circular) (Six-base) MAPSORT of: pFN21AE1591.seq Check: 3817 from: 1 to: 6091 pFN21AE1591 6091 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3087 3434 1937 Size: 1150 347 4594 Fragments arranged by size: 4594 1150 347 Acc65I G'GTAC_C Cuts at: 2058 3412 2058 Size: 1354 4737 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 3218 4154 5538 5806 1632 Size: 597 989 936 1384 268 1917 Fragments arranged by size: 1917 1384 989 936 597 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2370 2372 2653 3120 4990 5967 1203 Size: 1167 2 281 467 1870 977 1327 Fragments arranged by size: 1870 1327 1167 977 467 281 2 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 2025 2316 2953 3028 3834 5406 2025 Size: 291 637 75 806 1572 2710 Fragments arranged by size: 2710 1572 806 637 291 75 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2519 2840 3211 3416 1870 Size: 73 576 321 371 205 4545 Fragments arranged by size: 4545 576 371 321 205 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2058 2065 2509 3412 Size: 325 217 627 259 12 7 444 903 Cuts at: 3412 4867 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 903 627 444 325 259 217 12 7 BanII G_rGCy'C Cuts at: 1391 2565 3218 3410 1391 Size: 1174 653 192 4072 Fragments arranged by size: 4072 1174 653 192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5120 BbvCI CC'TCA_GC Cuts at: 3347 3347 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 3189 1548 Size: 1641 4450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2619 3176 4702 Size: 107 122 71 631 605 947 557 1526 Cuts at: 4702 136 Size: 1525 Fragments arranged by size: 1526 1525 947 631 605 557 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2990 3180 3508 2990 Size: 190 328 5573 Fragments arranged by size: 5573 328 190 Bme1580I G_kGCm'C Cuts at: 1790 2049 2223 2514 4152 5308 1790 Size: 259 174 291 1638 1156 2573 Fragments arranged by size: 2573 1638 1156 291 259 174 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2981 3050 3092 4751 1239 Size: 881 861 69 42 1659 2579 Fragments arranged by size: 2579 1659 881 861 69 42 Bpu10I CC'TnA_GC Cuts at: 3347 3347 Size: 6091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2771 4216 5081 5379 2771 Size: 1445 865 298 3483 Fragments arranged by size: 3483 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 3009 4754 882 Size: 617 684 826 1745 2219 Fragments arranged by size: 2219 1745 826 684 617 BsaAI yAC'GTr Cuts at: 493 1204 2373 3091 3882 493 Size: 711 1169 718 791 2702 Fragments arranged by size: 2702 1169 791 718 711 BsaBI GATnn'nnATC Cuts at: 3206 6085 3206 Size: 2879 3212 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2546 3898 4280 4915 Size: 53 83 186 936 1013 1352 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1451 1352 1013 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2883 4524 4922 5196 5343 5754 2883 Size: 1641 398 274 147 411 3220 Fragments arranged by size: 3220 1641 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 1178 Size: 30 165 30 603 30 5233 Fragments arranged by size: 5233 603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 1186 Size: 1588 4503 BseYI C'CCAG_C Cuts at: 2098 2176 2222 2617 3107 3341 3353 5294 5790 Size: 78 46 395 490 234 12 1941 496 Cuts at: 5790 2098 Size: 2399 Fragments arranged by size: 2399 1941 496 490 395 234 78 46 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2809 1163 Size: 573 1073 4445 Fragments arranged by size: 4445 1073 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 3541 4302 5330 1540 Size: 275 186 487 1053 761 1028 2301 Fragments arranged by size: 2301 1053 1028 761 487 275 186 BsiHKAI G_wGCw'C Cuts at: 2528 2565 4152 4237 5308 2528 Size: 37 1587 85 1071 3311 Fragments arranged by size: 3311 1587 1071 85 37 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 BsmBI CGTCTCn'nnnn_ Cuts at: 2972 2972 Size: 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2223 2514 2528 2565 3218 3410 Size: 399 259 174 291 14 37 653 192 Cuts at: 3410 4152 4237 5308 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 653 399 291 259 192 174 85 37 14 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3162 3446 844 Size: 2318 284 3489 Fragments arranged by size: 3489 2318 284 BsrBI CCG'CTC Cuts at: 1394 1823 3184 3276 3978 1394 Size: 429 1361 92 702 3507 Fragments arranged by size: 3507 1361 702 429 92 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2367 96 Size: 2271 3820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 2025 794 Size: 1231 4860 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2407 1184 Size: 1223 4868 BstYI r'GATC_y Cuts at: 1972 2820 3207 3421 3788 4173 4190 5642 5728 Size: 848 387 214 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 848 387 385 367 346 214 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2290 2290 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2749 487 Size: 683 56 1523 3829 Fragments arranged by size: 3829 1523 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DraIII CAC_nnn'GTG Cuts at: 2706 2706 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3375 3538 Size: 54 1425 50 275 18 177 1368 163 Cuts at: 3538 4427 6056 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1368 889 275 177 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 4021 1353 Size: 852 1816 3423 Fragments arranged by size: 3423 1816 852 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 4678 5052 5198 387 Size: 1064 138 741 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 741 374 146 138 EcoICRI GAG'CTC Cuts at: 2563 2563 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2229 2981 3050 3092 3218 4154 Size: 393 488 109 752 69 42 126 936 Cuts at: 4154 4751 5538 5806 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 936 787 752 597 488 393 268 126 109 69 42 EcoNI CCTnn'n_nnAGG Cuts at: 2786 2786 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 3025 3259 3025 Size: 234 5857 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 2353 2284 Size: 69 6022 FspI TGC'GCA Cuts at: 2579 4597 2579 Size: 2018 4073 HaeII r_GCGC'y Cuts at: 1678 2124 2316 2997 3080 3171 5238 1678 Size: 446 192 681 83 91 2067 2531 Fragments arranged by size: 2531 2067 681 446 192 91 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 4732 4764 4806 Size: 32 135 32 701 32 2427 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 2427 701 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 2062 3416 2062 Size: 1354 4737 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3225 3384 5205 5389 1305 Size: 1920 159 1821 184 2007 Fragments arranged by size: 2007 1920 1821 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4092 Fragments arranged by size: 4092 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2052 4049 4408 4567 518 Size: 648 886 1997 359 159 2042 Fragments arranged by size: 2042 1997 886 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2421 2617 2740 2950 3067 3333 3507 4184 Size: 815 196 123 210 117 266 174 677 Cuts at: 4184 5332 5577 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 815 677 266 245 210 196 174 123 117 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2464 3055 3124 3449 4994 1132 Size: 97 1235 591 69 325 1545 2229 Fragments arranged by size: 2229 1545 1235 591 325 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 3120 4990 3120 Size: 1870 4221 PflMI CCAn_nnn'nTGG Cuts at: 1108 2156 3028 1108 Size: 1048 872 4171 Fragments arranged by size: 4171 1048 872 PfoI T'CCnGG_A Cuts at: 3234 3234 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PmlI CAC'GTG Cuts at: 2373 2373 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2188 2220 2627 2659 4160 4192 5673 5705 2188 Size: 32 407 32 1501 32 1481 32 2574 Fragments arranged by size: 2574 1501 1481 407 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3025 3025 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2351 2383 2351 Size: 32 6059 PstI C_TGCA'G Cuts at: 830 2442 3060 3443 830 Size: 1612 618 383 3478 Fragments arranged by size: 3478 1612 618 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2617 2740 2950 3333 2617 Size: 123 210 383 5375 Fragments arranged by size: 5375 383 210 123 RsrII CG'GwC_CG Cuts at: 3040 3040 Size: 6091 SacI G_AGCT'C Cuts at: 2565 2565 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 2429 4339 6051 2429 Size: 1910 1712 2469 Fragments arranged by size: 2469 1910 1712 SexAI A'CCwGG_T Cuts at: 2925 2925 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2438 3056 3334 3439 4574 5255 5446 Size: 219 1393 618 278 105 1135 681 191 Cuts at: 5446 826 Size: 1471 Fragments arranged by size: 1471 1393 1135 681 618 278 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3213 3418 1872 Size: 1341 205 4545 Fragments arranged by size: 4545 1341 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 2786 4195 5096 5358 820 Size: 197 926 843 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 843 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 3171 3530 513 Size: 552 939 462 705 359 3074 Fragments arranged by size: 3074 939 705 552 462 359 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 2502 3395 4118 4135 4288 4473 Size: 1255 510 412 893 723 17 153 185 Cuts at: 4473 4918 325 Size: 445 1498 Fragments arranged by size: 1498 1255 893 723 510 445 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 2427 4337 Size: 274 80 33 51 1443 390 60 1910 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1910 1712 1443 390 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 2927 1316 Size: 1611 4480 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3211 3416 1870 Size: 1341 205 4545 Fragments arranged by size: 4545 1341 205 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI