Restriction map for
pFN21AE4369
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 625 GTCTAC GT^[AC][GT]AC
Alw44I 427 GTGCAC G^TGCAC
AlwI 1089 GATCC GGATC(4/5)
ApaLI 427 GTGCAC G^TGCAC
Asp700I 575 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 427 GTGCAC G[AT]GC[AT]^C
AvaI 674 CCCGGG C^[CT]CG[GA]G
AviII 861 TGCGCA TGC^GCA
BbsI 1144 GAAGAC GAAGAC(2/6)
BciVI 1017 GTATCC GTATCC(6/5)
BlpI 1069 GCTGAGC GC^T[ACGT]AGC
BmyI 427 GTGCAC G[AGT]GC[ACT]^C
BpmI 1057 CTGGAG CTGGAG(16/14)
Bpu1102I 1069 GCTGAGC GC^T[ACGT]AGC
BpuAI 1144 GAAGAC GAAGAC(2/6)
BsaI 134 GAGACC GGTCTC(1/5)
BsaWI 329 ACCGGA [AT]^CCGG[AT]
BseRI 16 CTCCTC GAGGAG(10/8)
BsiHKAI 427 GTGCAC G[AT]GC[AT]^C
BsmBI 1000 GAGACG CGTCTC(1/5)
BsoBI 674 CCCGGG C^[CT]CG[GA]G
Bsp1286I 427 GTGCAC G[AGT]GC[ACT]^C
BspMI 464 ACCTGC ACCTGC(4/8)
BsrI 1100 ACTGG ACTGG(1/-1)
BssSI 1009 CACGAG CACGAG(-5/-1)
BstBI 311 TTCGAA TT^CGAA
BstF5I 420 CATCC GGATG(2/0)
Cac8I 34 GCAAGC GC[ACGT]^[ACGT]GC
CelII 1069 GCTGAGC GC^T[ACGT]AGC
DraII 599 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1107 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1106 CCACGG C^C[GA][CT]GG
EcoO109I 599 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 420 CATCC GGATG(9/13)
FspI 861 TGCGCA TGC^GCA
HaeII 894 GGCGCT [GA]GCGC^[CT]
HgaI 1002 GACGC GACGC(5/10)
HincII 325 GTTGAC GT[CT]^[GA]AC
HindII 325 GTTGAC GT[CT]^[GA]AC
HphI 209 TCACC GGTGA(8/7)
MaeII 323 ACGT A^CGT
MaeIII 112 GTGAC ^GT[ACGT]AC
MseI 41 TTAA T^TAA
NspV 311 TTCGAA TT^CGAA
PpuMI 599 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 136 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1038 CTGCAG CTGCA^G
ScaI 971 AGTACT AGT^ACT
SfaNI 389 GCATC GCATC(5/9)
SfuI 311 TTCGAA TT^CGAA
SmaI 674 CCCGGG CCC^GGG
TaqI 312 TCGA T^CGA
Tru9I 41 TTAA T^TAA
Tsp45I 112 GTGAC ^GT[GC]AC
TthHB8I 312 TCGA T^CGA
XmaCI 674 CCCGGG C^CCGGG
XmaI 674 CCCGGG C^CCGGG
XmnI 575 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 387 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
474 CAGGAACTG
AvaII 600 GGACC G^G[AT]CC
1103 GGTCC
BsgI 8 CTGCAC GTGCAG(16/14)
541 CTGCAC
CfoI 862 GCGC GCG^C
895 GCGC
DpnII 451 GATC ^GATC
1089 GATC
EarI 534 GAAGAG CTCTTC(1/4)
813 GAAGAG
EcoRI 526 GAATTC G^AATTC
743 GAATTC
HhaI 862 GCGC GCG^C
895 GCGC
HinP1I 862 GCGC G^CGC
895 GCGC
Ksp632I 534 GAAGAG CTCTTC(1/4)
813 GAAGAG
MboI 451 GATC ^GATC
1089 GATC
MslI 420 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
870 CACTACTGTG
MspA1I 214 CAGCTG C[AC]G^C[GT]G
1047 CAGCTG
NdeII 451 GATC ^GATC
1089 GATC
NlaIV 369 GGTTCC GG[ACGT]^[ACGT]CC
770 GGCTCC
PleI 914 GACTC GAGTC(4/5)
283 GAGTC
PvuII 214 CAGCTG CAG^CTG
1047 CAGCTG
Sau3AI 451 GATC ^GATC
1089 GATC
SfcI 886 CTACAG C^T[GA][CT]AG
1038 CTGCAG
SmlI 499 CTTGAG C^T[CT][GA]AG
1094 CTCAAG
TfiI 178 GAATC G^A[AT]TC
396 GAATC
TspRI 162 CACTG CA[GC]TG[ACGT][ACGT]^
681 CACTG
XcmI 597 CCAGGACCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1046 CCAGCTGTCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI ApoI BalI
BbvI BcnI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BssKI BstNI
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeI MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NlaIII RsaI Sau96I ScrFI StyI
TseI Tsp509I