(Circular) (Six-base) MAPSORT of: pFN21AE4369.seq Check: 9671 from: 1 to: 5863 pFN21AE4369 5863 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3206 1937 Size: 694 575 4594 Fragments arranged by size: 4594 694 575 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 3168 3926 5310 5578 1632 Size: 840 696 758 1384 268 1917 Fragments arranged by size: 1917 1384 840 758 696 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 2879 1166 Size: 1713 4150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AlwNI CAG_nnn'CTG Cuts at: 2397 2484 3606 5178 2397 Size: 87 1122 1572 3082 Fragments arranged by size: 3082 1572 1122 87 ApaLI G'TGCA_C Cuts at: 2432 3920 5076 2432 Size: 1488 1156 3219 Fragments arranged by size: 3219 1488 1156 ApoI r'AATT_y Cuts at: 1493 2276 2531 2616 2748 3172 3398 3550 5659 Size: 783 255 85 132 424 226 152 2109 Cuts at: 5659 1493 Size: 1697 Fragments arranged by size: 2109 1697 783 424 255 226 152 132 85 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2679 3188 1870 Size: 73 736 509 4545 Fragments arranged by size: 4545 736 509 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 3182 1391 Size: 1791 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3156 928 Size: 971 1257 3635 Fragments arranged by size: 3635 1257 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3033 3755 4965 3033 Size: 722 1210 3931 Fragments arranged by size: 3931 1210 722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4474 136 Size: 107 122 71 631 605 2802 1525 Fragments arranged by size: 2802 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3075 3280 3075 Size: 205 5658 Bme1580I G_kGCm'C Cuts at: 1790 2436 3924 5080 1790 Size: 646 1488 1156 2573 Fragments arranged by size: 2573 1488 1156 646 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3594 4552 442 Size: 416 53 83 399 24 2177 958 1753 Fragments arranged by size: 2177 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2844 2844 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3083 4523 1239 Size: 1844 1440 2579 Fragments arranged by size: 2579 1844 1440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 3084 3988 4853 5151 2525 Size: 559 904 865 298 3237 Fragments arranged by size: 3237 904 865 559 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2133 4526 882 Size: 617 634 2393 2219 Fragments arranged by size: 2393 2219 634 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4296 4694 4968 5115 5526 2334 Size: 1962 398 274 147 411 2671 Fragments arranged by size: 2671 1962 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2007 2037 2096 2126 1178 Size: 30 165 30 604 30 59 30 4915 Fragments arranged by size: 4915 604 165 59 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 1186 Size: 826 5037 BseYI C'CCAG_C Cuts at: 2217 2221 3050 5066 5562 2217 Size: 4 829 2016 496 2518 Fragments arranged by size: 2518 2016 829 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1998 2531 1163 Size: 573 262 533 4495 Fragments arranged by size: 4495 573 533 262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 3924 4009 5080 2436 Size: 1488 85 1071 3219 Fragments arranged by size: 3219 1488 1071 85 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 BsmBI CGTCTCn'nnnn_ Cuts at: 2999 2999 Size: 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2436 3182 3924 4009 5080 1391 Size: 399 646 746 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 646 399 85 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 3218 844 Size: 1634 740 3489 Fragments arranged by size: 3489 1634 740 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4507 4694 1700 Size: 110 2697 187 2869 Fragments arranged by size: 2869 2697 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3014 3923 4935 3014 Size: 909 1012 3942 Fragments arranged by size: 3942 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5863 BstBI TT'CG_AA Cuts at: 2317 4667 2317 Size: 2350 3513 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3111 513 Size: 552 2046 3265 Fragments arranged by size: 3265 2046 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DraIII CAC_nnn'GTG Cuts at: 3117 3117 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2192 2206 Size: 54 1425 50 275 18 198 164 14 Cuts at: 2206 2599 3310 4199 5828 8 Size: 393 711 889 1629 43 Fragments arranged by size: 1629 1425 889 711 393 275 198 164 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2813 3793 1353 Size: 1181 279 980 3423 Fragments arranged by size: 3423 1181 980 279 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2472 3083 3168 3926 4523 5310 5578 Size: 393 840 611 85 758 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 840 787 758 611 597 393 268 85 EcoO109I rG'GnC_Cy Cuts at: 2605 2605 Size: 5863 EcoRI G'AATT_C Cuts at: 2531 2748 3172 3550 2531 Size: 217 424 378 4844 Fragments arranged by size: 4844 424 378 217 FspI TGC'GCA Cuts at: 2868 4369 2868 Size: 1501 4362 HaeII r_GCGC'y Cuts at: 1678 2903 5010 1678 Size: 1225 2107 2531 Fragments arranged by size: 2531 2107 1225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2980 3012 4504 4536 4578 Size: 32 135 32 1408 32 1492 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1492 1408 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 3207 3453 669 Size: 988 281 394 875 246 3079 Fragments arranged by size: 3079 988 875 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2194 2208 2601 10 Size: 54 1425 343 198 164 14 393 3272 Fragments arranged by size: 3272 1425 393 343 198 164 54 14 MslI CAynn'nnrTG Cuts at: 518 1166 2429 2879 3821 4180 4339 518 Size: 648 1263 450 942 359 159 2042 Fragments arranged by size: 2042 1263 942 648 450 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 3054 3279 3956 5104 5349 1606 Size: 615 833 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 833 677 615 245 225 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5311 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 3221 4766 1132 Size: 97 1992 1545 2229 Fragments arranged by size: 2229 1992 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2605 Size: 5863 PshAI GACnn'nnGTC Cuts at: 1613 2145 1613 Size: 532 5331 PsiI TTA'TAA Cuts at: 2556 3433 2556 Size: 877 4986 PstI C_TGCA'G Cuts at: 830 3047 3215 830 Size: 2217 168 3478 Fragments arranged by size: 3478 2217 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2221 3054 2221 Size: 833 5030 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 2978 4111 5823 2978 Size: 1133 1712 3018 Fragments arranged by size: 3018 1712 1133 SfcI C'TryA_G Cuts at: 826 1045 2891 3043 3211 4346 5027 5218 826 Size: 219 1846 152 168 1135 681 191 1471 Fragments arranged by size: 1846 1471 1135 681 219 191 168 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 2681 3190 1872 Size: 809 509 4545 Fragments arranged by size: 4545 809 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 3099 3967 4868 5130 820 Size: 197 926 561 595 868 901 262 1553 Fragments arranged by size: 1553 926 901 868 595 561 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2050 2100 2574 2779 3302 513 Size: 552 985 50 474 205 523 3074 Fragments arranged by size: 3074 985 552 523 474 205 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 2157 2231 3890 3907 4060 4245 Size: 1255 520 57 74 1659 17 153 185 Cuts at: 4245 4690 325 Size: 445 1498 Fragments arranged by size: 1659 1498 1255 520 445 185 153 74 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 2958 2976 Size: 274 80 33 51 1443 115 866 18 Cuts at: 2976 2991 3155 4109 5821 96 Size: 15 164 954 1712 138 Fragments arranged by size: 1712 1443 954 866 274 164 138 115 80 51 33 18 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 5823 5855 2007 Size: 32 3784 32 2015 Fragments arranged by size: 3784 2015 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2609 3058 2609 Size: 449 5414 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 2679 3188 1870 Size: 809 509 4545 Fragments arranged by size: 4545 809 509 XmnI GAAnn'nnTTC Cuts at: 2584 3992 2584 Size: 1408 4455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI