Restriction map for
pFN21AE9664
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BclI BfaI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MaeI MfeI MluI
MluNI MroI MscI MunI MvnI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AlwNI 71 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BcgI 605 CGATAGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 222 GTATCC GTATCC(6/5)
BcnI 297 CCGGG CC^[GC]GG
BglII 62 AGATCT A^GATCT
BsaJI 1256 CCCAGG C^C[ACGT][ACGT]GG
BsgI 877 GTGCAG GTGCAG(16/14)
BsiYI 951 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 951 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1254 GTCCC GGGAC(10/14)
BstYI 62 AGATCT [GA]^GATC[CT]
DraII 902 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 902 AGGACCT [GA]G^G[ACGT]CC[CT]
HapII 297 CCGG C^CGG
HindIII 790 AAGCTT A^AGCTT
HpaII 297 CCGG C^CGG
MaeII 409 ACGT A^CGT
MflI 62 AGATCT [GA]^GATC[CT]
MslI 1087 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1355 CAGCTG C[AC]G^C[GT]G
MspI 297 CCGG C^CGG
NciI 297 CCGGG CC^[GC]GG
NspI 397 GCATGC [GA]CATG^[CT]
PpuMI 902 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1355 CAGCTG CAG^CTG
SapI 523 GAAGAGC GCTCTTC(1/4)
SphI 397 GCATGC GCATG^C
TaqI 177 TCGA T^CGA
TspRI 203 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 177 TCGA T^CGA
XcmI 803 CCAGACAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 62 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 470 CCGC CCGC(-3/-1)
445 GCGG
AseI 486 ATTAAT AT^TAAT
1180 ATTAAT
AsnI 486 ATTAAT AT^TAAT
1180 ATTAAT
Asp700I 1261 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGATGTTC
BbsI 1002 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BpmI 740 CTCCAG CTGGAG(16/14)
496 CTGGAG
BpuAI 1002 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BsaBI 103 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
589 GATTTTCATC
BstNI 477 CCAGG CC^[AT]GG
1257 CCAGG
Cac8I 397 GCATGC GC[ACGT]^[ACGT]GC
854 GCCTGC
EcoRII 477 CCAGG ^CC[AT]GG
1257 CCAGG
HincII 847 GTTGAC GT[CT]^[GA]AC
1386 GTCAAC
HindII 847 GTTGAC GT[CT]^[GA]AC
1386 GTCAAC
HphI 300 GGTGA GGTGA(8/7)
92 TCACC
MaeIII 265 GTTAC ^GT[ACGT]AC
1171 GTAAC
MamI 103 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
589 GATTTTCATC
MvaI 477 CCAGG CC^[AT]GG
1257 CCAGG
PshBI 486 ATTAAT AT^TAAT
1180 ATTAAT
SfaNI 198 GATGC GCATC(5/9)
1228 GATGC
SspI 643 AATATT AAT^ATT
1263 AATATT
XmnI 1261 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGATGTTC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BsmAI BsrI BssKI Cfr13I DdeI
DpnII EarI EcoRI Fnu4HI HinfI
ItaI Ksp632I MboI MboII MnlI
MseI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I