(Circular) (Six-base) MAPSORT of: pFN21AE9664.seq Check: 8153 from: 1 to: 6097 pFN21AE9664 6097 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2238 2377 3017 3033 3275 3389 4160 Size: 519 87 139 640 16 242 114 771 Cuts at: 4160 5544 5812 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 771 640 519 268 242 139 114 87 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 2081 3840 5412 2081 Size: 1759 1572 2766 Fragments arranged by size: 2766 1759 1572 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2178 2423 2562 2629 3019 3216 3406 3632 Size: 685 245 139 67 390 197 190 226 Cuts at: 3632 3784 5893 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 685 390 245 226 197 190 152 139 67 AseI AT'TA_AT Cuts at: 160 1032 2492 3186 4651 160 Size: 872 1460 694 1465 1606 Fragments arranged by size: 1606 1465 1460 872 694 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3422 1870 Size: 73 1479 4545 Fragments arranged by size: 4545 1479 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3418 4873 618 Size: 325 217 627 1631 1455 1842 Fragments arranged by size: 1842 1631 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3416 1391 Size: 2025 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3014 3362 928 Size: 971 1115 348 3663 Fragments arranged by size: 3663 1115 971 348 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 4288 4322 1903 Size: 34 662 34 1655 34 3678 Fragments arranged by size: 3678 1655 662 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2238 3989 5199 2238 Size: 1751 1210 3136 Fragments arranged by size: 3136 1751 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 6092 2067 Size: 4025 2072 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2730 4757 1239 Size: 1283 208 2027 2579 Fragments arranged by size: 2579 2027 1283 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 2112 2598 6091 2112 Size: 486 3493 2118 Fragments arranged by size: 3493 2118 486 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2835 2865 1178 Size: 30 165 30 1432 30 4410 Fragments arranged by size: 4410 1432 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6097 BseYI C'CCAG_C Cuts at: 5300 5796 5300 Size: 496 5601 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 1163 Size: 573 1167 4357 Fragments arranged by size: 4357 1167 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4158 4243 5314 4158 Size: 85 1071 4941 Fragments arranged by size: 4941 1071 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3416 4158 4243 5314 1391 Size: 399 1626 742 85 1071 2174 Fragments arranged by size: 2174 1626 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2067 3427 3794 4179 4196 5648 5734 5746 Size: 95 1360 367 385 17 1452 86 12 Cuts at: 5746 6092 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1360 385 367 346 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2718 2753 529 Size: 35 2154 35 3873 Fragments arranged by size: 3873 2154 35 35 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3544 4433 6062 Size: 54 1425 50 275 18 1714 889 1629 Cuts at: 6062 8 Size: 43 Fragments arranged by size: 1714 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2523 2532 3365 4027 1353 Size: 1170 9 833 662 3423 Fragments arranged by size: 3423 1170 833 662 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2238 2377 2522 2730 3017 3033 Size: 393 519 87 139 145 208 287 16 Cuts at: 3033 3275 3389 4160 4757 5544 5812 1239 Size: 242 114 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 519 393 287 268 242 208 145 139 114 87 16 EcoO109I rG'GnC_Cy Cuts at: 2908 2908 Size: 6097 EcoRI G'AATT_C Cuts at: 2178 2562 3216 3406 3784 2178 Size: 384 654 190 378 4491 Fragments arranged by size: 4491 654 384 378 190 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3147 3179 4738 4770 4812 Size: 32 135 32 1575 32 1559 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1575 1559 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2854 3393 3441 3687 669 Size: 988 281 916 539 48 246 3079 Fragments arranged by size: 3079 988 916 539 281 246 48 HindIII A'AGCT_T Cuts at: 748 2795 748 Size: 2047 4050 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2856 5211 5395 1305 Size: 1551 2355 184 2007 Fragments arranged by size: 2355 2007 1551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3096 4055 4414 4573 518 Size: 648 1930 959 359 159 2042 Fragments arranged by size: 2042 1930 959 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3362 3513 4190 5338 5583 1606 Size: 1756 151 677 1148 245 2120 Fragments arranged by size: 2120 1756 1148 677 245 151 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2406 3455 5000 1132 Size: 97 1177 1049 1545 2229 Fragments arranged by size: 2229 1545 1177 1049 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2908 2908 Size: 6097 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 2766 3667 2766 Size: 901 5196 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 3362 3362 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2523 2523 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 3445 4580 5261 5452 826 Size: 219 2400 1135 681 191 1471 Fragments arranged by size: 2400 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 2406 3455 1132 Size: 1274 1049 3774 Fragments arranged by size: 3774 1274 1049 SspI AAT'ATT Cuts at: 5 52 2650 3270 4021 5 Size: 47 2598 620 751 2081 Fragments arranged by size: 2598 2081 751 620 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 3536 513 Size: 552 939 1532 3074 Fragments arranged by size: 3074 1532 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4124 4141 4294 4479 4924 325 Size: 1255 2544 17 153 185 445 1498 Fragments arranged by size: 2544 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2815 2815 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 3270 3380 4226 3270 Size: 110 846 5141 Fragments arranged by size: 5141 846 110 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI