Restriction map for
pFN21AE2162
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AvaII AviII AvrII BamHI BanII
BbeI BbvCI BcgI BclI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 650 CGCG CG^CG
AcyI 688 GACGCC G[GA]^CG[CT]C
AhdI 162 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 162 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 771 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 485 CCCGAG C^[CT]CG[GA]G
BalI 813 TGGCCA TGG^CCA
BanI 244 GGCACC G^G[CT][GA]CC
BbrPI 275 CACGTG CAC^GTG
BbsI 49 GTCTTC GAAGAC(2/6)
BciVI 736 GGATAC GTATCC(6/5)
BcnI 414 CCGGG CC^[GC]GG
BlpI 134 GCTCAGC GC^T[ACGT]AGC
BpmI 717 CTCCAG CTGGAG(16/14)
Bpu1102I 134 GCTCAGC GC^T[ACGT]AGC
BpuAI 49 GTCTTC GAAGAC(2/6)
BsaHI 688 GACGCC G[GA]^CG[CT]C
BsiYI 313 CCTAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 313 CCTAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 559 GGGAC GGGAC(10/14)
BsoBI 485 CCCGAG C^[CT]CG[GA]G
BsrDI 396 CATTGC GCAATG(2/0)
BsrFI 451 GCCGGC [GA]^CCGG[CT]
BstUI 650 CGCG CG^CG
BstXI 816 CCACAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 134 GCTCAGC GC^T[ACGT]AGC
CfoI 651 GCGC GCG^C
Cfr10I 451 GCCGGC [GA]^CCGG[CT]
Cfr13I 403 GGGCC G^G[ACGT]CC
Eam1105I 162 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 782 CTCTTC CTCTTC(1/4)
EcoRI 529 GAATTC G^AATTC
EcoT14I 56 CCAAGG C^C[AT][AT]GG
FauI 357 GCGGG CCCGC(4/6)
HgaI 688 GACGC GACGC(5/10)
HhaI 651 GCGC GCG^C
Hin1I 688 GACGCC G[GA]^CG[CT]C
HinP1I 651 GCGC G^CGC
Ksp632I 782 CTCTTC CTCTTC(1/4)
MluNI 813 TGGCCA TGG^CCA
MnlI 439 GAGG CCTC(7/6)
MscI 813 TGGCCA TGG^CCA
MspA1I 611 CCGCTG C[AC]G^C[GT]G
MvnI 650 CGCG CG^CG
MwoI 625 GCCTGGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 451 GCCGGC GCC^GGC
NciI 414 CCGGG CC^[GC]GG
NgoMIV 451 GCCGGC G^CCGGC
NlaIII 384 CATG CATG^
PflFI 771 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 747 GACTC GAGTC(4/5)
PmaCI 275 CACGTG CAC^GTG
PmlI 275 CACGTG CAC^GTG
PshAI 609 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 781 GCTCTTC GCTCTTC(1/4)
Sau96I 403 GGGCC G^G[ACGT]CC
StyI 56 CCAAGG C^C[AT][AT]GG
TfiI 298 GATTC G^A[AT]TC
Tth111I 771 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 361 GAGCAC G[AT]GC[AT]^C
419 GTGCTC
BbvI 664 GCAGC GCAGC(8/12)
377 GCTGC
BmyI 361 GAGCAC G[AGT]GC[ACT]^C
419 GTGCTC
BsaAI 15 CACGTA [CT]AC^GT[GA]
275 CACGTG
BsaI 148 GAGACC GGTCTC(1/5)
607 GAGACC
BsiHKAI 361 GAGCAC G[AT]GC[AT]^C
419 GTGCTC
Bsp1286I 361 GAGCAC G[AGT]GC[ACT]^C
419 GTGCTC
BsrI 319 ACTGG ACTGG(1/-1)
834 ACTGG
DpnII 25 GATC ^GATC
265 GATC
EaeI 449 TGGCCG [CT]^GGCC[GA]
813 TGGCCA
HapII 414 CCGG C^CGG
452 CCGG
HindIII 82 AAGCTT A^AGCTT
820 AAGCTT
HinfI 298 GATTC G^A[ACGT]TC
747 GACTC
HpaII 414 CCGG C^CGG
452 CCGG
HphI 53 TCACC GGTGA(8/7)
310 TCACC
MboI 25 GATC ^GATC
265 GATC
MspI 414 CCGG C^CGG
452 CCGG
NdeII 25 GATC ^GATC
265 GATC
NlaIV 244 GGCACC GG[ACGT]^[ACGT]CC
574 GGCTCC
Sau3AI 25 GATC ^GATC
265 GATC
TaqI 296 TCGA T^CGA
392 TCGA
TseI 377 GCTGC G^C[AT]GC
664 GCAGC
Tsp45I 160 GTGAC ^GT[GC]AC
490 GTGAC
TthHB8I 296 TCGA T^CGA
392 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BfaI BsaJI BsmAI BssKI BstF5I
BstNI Cac8I DdeI EcoRII Fnu4HI
FokI HaeIII ItaI MaeI MaeII
MaeIII MboII MseI MslI MvaI
RsaI ScrFI Tru9I Tsp509I TspRI