(Linear) (Six-base) MAPSORT of: KIEE4501.seq Check: 3380 from: 1 to: 1581 KIEE4501.orf With 182 enzymes: SgfI * March 22, 2012 18:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 831 1525 1581 Size: 831 694 56 Fragments arranged by size: 831 694 56 AflIII A'CryG_T Cuts at: 0 35 1581 Size: 35 1546 AlwNI CAG_nnn'CTG Cuts at: 0 1309 1534 1581 Size: 1309 225 47 Fragments arranged by size: 1309 225 47 AseI AT'TA_AT Cuts at: 0 809 1581 Size: 809 772 AvaI C'yCGr_G Cuts at: 0 131 1387 1581 Size: 131 1256 194 Fragments arranged by size: 1256 194 131 BamHI G'GATC_C Cuts at: 0 1144 1581 Size: 1144 437 BanI G'GyrC_C Cuts at: 0 328 607 1581 Size: 328 279 974 Fragments arranged by size: 974 328 279 BanII G_rGCy'C Cuts at: 0 479 1127 1581 Size: 479 648 454 Fragments arranged by size: 648 479 454 BbeI G_GCGC'C Cuts at: 0 332 1581 Size: 332 1249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 550 584 1581 Size: 550 34 997 Fragments arranged by size: 997 550 34 BclI T'GATC_A Cuts at: 0 971 1581 Size: 971 610 BlpI GC'TnA_GC Cuts at: 0 1085 1581 Size: 1085 496 Bme1580I G_kGCm'C Cuts at: 0 610 1303 1581 Size: 610 693 278 Fragments arranged by size: 693 610 278 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1221 1581 Size: 1221 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1373 1581 Size: 1373 208 BsaI GGTCTCn'nnnn_ Cuts at: 0 698 1390 1581 Size: 698 692 191 Fragments arranged by size: 698 692 191 BsaAI yAC'GTr Cuts at: 0 186 804 1581 Size: 186 618 777 Fragments arranged by size: 777 618 186 BsaHI Gr'CG_yC Cuts at: 0 329 1581 Size: 329 1252 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 191 221 695 725 1581 Size: 191 30 474 30 856 Fragments arranged by size: 856 474 191 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 688 1210 1581 Size: 688 522 371 Fragments arranged by size: 688 522 371 BseYI C'CCAG_C Cuts at: 0 249 1140 1581 Size: 249 891 441 Fragments arranged by size: 891 441 249 BsiHKAI G_wGCw'C Cuts at: 0 479 1127 1581 Size: 479 648 454 Fragments arranged by size: 648 479 454 BsmBI CGTCTCn'nnnn_ Cuts at: 0 292 763 1581 Size: 292 471 818 Fragments arranged by size: 818 471 292 Bsp1286I G_dGCh'C Cuts at: 0 479 610 1127 1303 1581 Size: 479 131 517 176 278 Fragments arranged by size: 517 479 278 176 131 BspHI T'CATG_A Cuts at: 0 1244 1581 Size: 1244 337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1490 1581 Size: 1490 91 BsrBI CCG'CTC Cuts at: 0 1325 1581 Size: 1325 256 BsrDI GCAATG_nn' Cuts at: 0 353 1581 Size: 353 1228 BsrGI T'GTAC_A Cuts at: 0 1553 1581 Size: 1553 28 BstEII G'GTnAC_C Cuts at: 0 1395 1581 Size: 1395 186 BstXI CCAn_nnnn'nTGG Cuts at: 0 344 1140 1581 Size: 344 796 441 Fragments arranged by size: 796 441 344 BstYI r'GATC_y Cuts at: 0 664 1144 1581 Size: 664 480 437 Fragments arranged by size: 664 480 437 BtgI C'CryG_G Cuts at: 0 1277 1573 1581 Size: 1277 296 8 Fragments arranged by size: 1277 296 8 BtsI GCAGTG_nn' Cuts at: 0 604 1033 1581 Size: 604 429 548 Fragments arranged by size: 604 548 429 DraIII CAC_nnn'GTG Cuts at: 0 564 1319 1581 Size: 564 755 262 Fragments arranged by size: 755 564 262 EaeI y'GGCC_r Cuts at: 0 639 1581 Size: 639 942 EarI CTCTTCn'nnn_ Cuts at: 0 1172 1581 Size: 1172 409 EcoICRI GAG'CTC Cuts at: 0 477 1125 1581 Size: 477 648 456 Fragments arranged by size: 648 477 456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 831 1373 1525 1581 Size: 831 542 152 56 Fragments arranged by size: 831 542 152 56 EcoRV GAT'ATC Cuts at: 0 1410 1581 Size: 1410 171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 861 954 1581 Size: 861 93 627 Fragments arranged by size: 861 627 93 HaeII r_GCGC'y Cuts at: 0 332 1581 Size: 332 1249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1233 1265 1581 Size: 1233 32 316 Fragments arranged by size: 1233 316 32 HincII GTy'rAC Cuts at: 0 741 1273 1581 Size: 741 532 308 Fragments arranged by size: 741 532 308 HindIII A'AGCT_T Cuts at: 0 1154 1581 Size: 1154 427 KasI G'GCGC_C Cuts at: 0 328 1581 Size: 328 1253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 123 439 847 1581 Size: 123 316 408 734 Fragments arranged by size: 734 408 316 123 MscI TGG'CCA Cuts at: 0 641 1581 Size: 641 940 MslI CAynn'nnrTG Cuts at: 0 342 1581 Size: 342 1239 MspA1I CmG'CkG Cuts at: 0 888 1447 1581 Size: 888 559 134 Fragments arranged by size: 888 559 134 NarI GG'CG_CC Cuts at: 0 329 1581 Size: 329 1252 NspI r_CATG'y Cuts at: 0 39 178 445 1581 Size: 39 139 267 1136 Fragments arranged by size: 1136 267 139 39 PasI CC'CwG_GG Cuts at: 0 428 1539 1581 Size: 428 1111 42 Fragments arranged by size: 1111 428 42 PciI A'CATG_T Cuts at: 0 35 1581 Size: 35 1546 PflMI CCAn_nnn'nTGG Cuts at: 0 1319 1581 Size: 1319 262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1471 1503 1581 Size: 1471 32 78 Fragments arranged by size: 1471 78 32 SacI G_AGCT'C Cuts at: 0 479 1127 1581 Size: 479 648 454 Fragments arranged by size: 648 479 454 ScaI AGT'ACT Cuts at: 0 1204 1581 Size: 1204 377 SfoI GGC'GCC Cuts at: 0 330 1581 Size: 330 1251 SnaBI TAC'GTA Cuts at: 0 186 804 1581 Size: 186 618 777 Fragments arranged by size: 777 618 186 StuI AGG'CCT Cuts at: 0 937 1581 Size: 937 644 StyI C'CwwG_G Cuts at: 0 1007 1581 Size: 1007 574 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1414 1474 1581 Size: 1414 60 107 Fragments arranged by size: 1414 107 60 TatI w'GTAC_w Cuts at: 0 1202 1553 1581 Size: 1202 351 28 Fragments arranged by size: 1202 351 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 191 223 695 727 1581 Size: 191 32 472 32 854 Fragments arranged by size: 854 472 191 32 32 Tth111I GACn'n_nGTC Cuts at: 0 46 1581 Size: 46 1535 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 360 1581 Size: 360 1221 Enzymes that do cut: AcuI AflIII AlwNI AseI AvaI BamHI BanI BanII BbeI BcgI BclI BlpI Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NspI PasI PciI PflMI PpiI SacI ScaI SfoI SnaBI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BbsI BbvCI BciVI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI