################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsmBI BspDI BspHI BspLU11I BspMI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcyI 507 GGCGTC G[GA]^CG[CT]C Alw44I 1468 GTGCAC G^TGCAC ApaLI 1468 GTGCAC G^TGCAC AvaI 1201 CTCGGG C^[CT]CG[GA]G BamHI 1352 GGATCC G^GATCC BbsI 598 GAAGAC GAAGAC(2/6) BciVI 1392 GGATAC GTATCC(6/5) BpmI 1769 CTCCAG CTGGAG(16/14) BpuAI 598 GAAGAC GAAGAC(2/6) BsaBI 1299 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 507 GGCGTC G[GA]^CG[CT]C BsaI 63 GGTCTC GGTCTC(1/5) BsgI 978 CTGCAC GTGCAG(16/14) BsiWI 1232 CGTACG C^GTACG BsoBI 1201 CTCGGG C^[CT]CG[GA]G Bsp1407I 1301 TGTACA T^GTACA BsrDI 522 CATTGC GCAATG(2/0) BsrGI 1301 TGTACA T^GTACA BstXI 435 CCAGGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 1528 CGGACCG CG^G[AT]CCG DraII 213 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 590 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 213 GGGACCC [GA]G^G[ACGT]CC[CT] HaeII 1188 GGCGCT [GA]GCGC^[CT] Hin1I 507 GGCGTC G[GA]^CG[CT]C HindIII 1721 AAGCTT A^AGCTT HphI 1143 GGTGA GGTGA(8/7) MaeII 1061 ACGT A^CGT MamI 1299 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1330 GCCGGC GCC^GGC NgoMIV 1330 GCCGGC G^CCGGC PpuMI 213 GGGACCC [GA]G^G[AT]CC[CT] PshAI 684 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1528 CGGACCG CG^G[AT]CCG SphI 1326 GCATGC GCATG^C SspBI 1301 TGTACA T^GTACA SspI 661 AATATT AAT^ATT XcmI 833 CCAAGATACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 72 CGCG CG^CG 1643 CGCG AccIII 575 TCCGGA T^CCGGA 1295 TCCGGA Asp700I 1146 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1734 GAATCTCTTC AviII 23 TGCGCA TGC^GCA 89 TGCGCA AvrII 132 CCTAGG C^CTAGG 309 CCTAGG BcnI 51 CCGGG CC^[GC]GG 512 CCGGG BlnI 132 CCTAGG C^CTAGG 309 CCTAGG BsaWI 575 TCCGGA [AT]^CCGG[AT] 1295 TCCGGA BseAI 575 TCCGGA T^CCGGA 1295 TCCGGA BsmI 730 GCATTC GAATGC(1/-1) 791 GCATTC BspEI 575 TCCGGA T^CCGGA 1295 TCCGGA BsrBI 1653 CCGCTC CCGCTC(-3/-3) 3 GAGCGG BssSI 1687 CACGAG CACGAG(-5/-1) 41 CTCGTG BstUI 72 CGCG CG^CG 1643 CGCG EcoNI 855 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 977 CCTGCACAAGG FspI 23 TGCGCA TGC^GCA 89 TGCGCA HgaI 508 GCGTC GACGC(5/10) 926 GCGTC MroI 575 TCCGGA T^CCGGA 1295 TCCGGA MvnI 72 CGCG CG^CG 1643 CGCG NciI 51 CCGGG CC^[GC]GG 512 CCGGG NcoI 1139 CCATGG C^CATGG 1458 CCATGG NspI 1326 GCATGC [GA]CATG^[CT] 1858 ACATGC PstI 1192 CTGCAG CTGCA^G 1321 CTGCAG PvuII 1579 CAGCTG CAG^CTG 1753 CAGCTG SapI 303 GAAGAGC GCTCTTC(1/4) 15 GCTCTTC SfcI 1192 CTGCAG C^T[GA][CT]AG 1321 CTGCAG TaqI 1130 TCGA T^CGA 1274 TCGA TthHB8I 1130 TCGA T^CGA 1274 TCGA XmnI 1146 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1734 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BanII BbvI BfaI BmyI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII