(Circular) (Six-base) MAPSORT of: pFN21AE6427.seq Check: 9777 from: 1 to: 6568 pFN21AE6427 6568 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2262 3614 3730 4631 6015 6283 1632 Size: 630 1352 116 901 1384 268 1917 Fragments arranged by size: 1917 1384 1352 901 630 268 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2554 1184 Size: 1370 5198 AlwNI CAG_nnn'CTG Cuts at: 4311 5883 4311 Size: 1572 4996 ApaLI G'TGCA_C Cuts at: 3473 4625 5781 3473 Size: 1152 1156 4260 Fragments arranged by size: 4260 1156 1152 ApoI r'AATT_y Cuts at: 1493 2231 3017 3038 3877 4103 4255 6364 1493 Size: 738 786 21 839 226 152 2109 1697 Fragments arranged by size: 2109 1697 839 786 738 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 3206 3893 1870 Size: 73 1263 687 4545 Fragments arranged by size: 4545 1263 687 73 AvrII C'CTAG_G Cuts at: 2137 2314 2137 Size: 177 6391 BamHI G'GATC_C Cuts at: 3357 3898 4265 3357 Size: 541 367 5660 Fragments arranged by size: 5660 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3889 5344 618 Size: 325 217 627 2102 1455 1842 Fragments arranged by size: 2102 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2807 3678 3887 1391 Size: 631 785 871 209 4072 Fragments arranged by size: 4072 871 785 631 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 928 Size: 971 711 4886 Fragments arranged by size: 4886 971 711 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3391 4460 5670 3391 Size: 1069 1210 4289 Fragments arranged by size: 4289 1210 1069 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6563 6563 Size: 6568 BlpI GC'TnA_GC Cuts at: 3985 3985 Size: 6568 Bme1580I G_kGCm'C Cuts at: 1790 3477 3847 4629 5785 1790 Size: 1687 370 782 1156 2573 Fragments arranged by size: 2573 1687 1156 782 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4299 5257 442 Size: 416 53 83 399 24 2882 958 1753 Fragments arranged by size: 2882 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3759 5228 1239 Size: 2520 1469 2579 Fragments arranged by size: 2579 2520 1469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 2933 3842 4693 5558 5856 2438 Size: 495 909 851 865 298 3150 Fragments arranged by size: 3150 909 865 851 495 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 5231 882 Size: 617 575 3157 2219 Fragments arranged by size: 3157 2219 617 575 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 3308 6562 3308 Size: 3254 3314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2513 4375 4757 5392 Size: 53 83 186 936 980 1862 382 635 Cuts at: 5392 275 Size: 1451 Fragments arranged by size: 1862 1451 980 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2580 3300 5001 5399 5673 5820 6231 2580 Size: 720 1701 398 274 147 411 2917 Fragments arranged by size: 2917 1701 720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3537 3567 1178 Size: 30 165 30 2134 30 4179 Fragments arranged by size: 4179 2134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 3260 3524 3593 1186 Size: 1309 765 264 69 4161 Fragments arranged by size: 4161 1309 765 264 69 BseYI C'CCAG_C Cuts at: 2939 3810 5771 6267 2939 Size: 871 1961 496 3240 Fragments arranged by size: 3240 1961 871 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2968 1163 Size: 573 1232 4763 Fragments arranged by size: 4763 1232 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3340 3532 3639 4018 4779 Size: 275 186 913 426 192 107 379 761 Cuts at: 4779 5807 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 913 761 426 379 275 192 186 107 BsiHKAI G_wGCw'C Cuts at: 2526 3389 3477 4629 4714 5785 2526 Size: 863 88 1152 85 1071 3309 Fragments arranged by size: 3309 1152 1071 863 88 85 BsiWI C'GTAC_G Cuts at: 3237 3237 Size: 6568 BsmI GAATG_Cn' Cuts at: 2735 2796 4079 4172 2735 Size: 61 1283 93 5131 Fragments arranged by size: 5131 1283 93 61 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2526 2807 3389 3477 3678 3847 Size: 399 232 504 281 582 88 201 169 Cuts at: 3847 3887 4629 4714 5785 1391 Size: 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 504 399 281 232 201 169 88 85 40 BspEI T'CCGG_A Cuts at: 2580 3300 6231 2580 Size: 720 2931 2917 Fragments arranged by size: 2931 2917 720 BspHI T'CATG_A Cuts at: 4457 6187 4457 Size: 1730 4838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3923 844 Size: 3079 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3660 4455 1394 Size: 429 187 1650 795 3507 Fragments arranged by size: 3507 1650 795 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2526 5063 5237 65 Size: 1183 1278 2537 174 1396 Fragments arranged by size: 2537 1396 1278 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 2913 3335 5212 5399 1700 Size: 110 272 831 422 1877 187 2869 Fragments arranged by size: 2869 1877 831 422 272 187 110 BsrGI T'GTAC_A Cuts at: 96 3306 96 Size: 3210 3358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 2046 3692 4628 5640 2046 Size: 1646 936 1012 2974 Fragments arranged by size: 2974 1646 1012 936 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6568 BstBI TT'CG_AA Cuts at: 5372 5372 Size: 6568 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstXI CCAn_nnnn'nTGG Cuts at: 2447 2447 Size: 6568 BstYI r'GATC_y Cuts at: 1972 3201 3357 3816 3898 4265 4650 4667 6119 Size: 1229 156 459 82 367 385 17 1452 Cuts at: 6119 6205 6217 6563 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1229 459 385 367 346 156 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 2223 3144 3463 3537 513 Size: 552 969 189 921 319 74 3544 Fragments arranged by size: 3544 969 921 552 319 189 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2580 3295 487 Size: 683 56 1354 715 3760 Fragments arranged by size: 3760 1354 715 683 56 BtsI GCAGTG_nn' Cuts at: 3185 3545 4084 4878 4898 3185 Size: 360 539 794 20 4855 Fragments arranged by size: 4855 794 539 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DraIII CAC_nnn'GTG Cuts at: 2600 2600 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2661 2889 2911 Size: 54 1425 50 275 18 831 228 22 Cuts at: 2911 3337 3406 3529 3603 3636 3705 4015 4904 Size: 426 69 123 74 33 69 310 889 Cuts at: 4904 6533 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 831 426 310 275 228 123 74 69 69 54 50 43 33 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 3337 3529 3636 4015 1537 Size: 275 1099 426 192 107 379 4090 Fragments arranged by size: 4090 1099 426 379 275 192 107 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2238 2303 3749 4498 1353 Size: 674 211 65 1446 749 3423 Fragments arranged by size: 3423 1446 749 674 211 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 3614 3730 3759 4631 5228 6015 Size: 393 630 1352 116 29 872 597 787 Cuts at: 6015 6283 1239 Size: 268 1524 Fragments arranged by size: 1524 1352 872 787 630 597 393 268 116 29 EcoNI CCTnn'n_nnAGG Cuts at: 2864 2986 2864 Size: 122 6446 EcoO109I rG'GnC_Cy Cuts at: 2219 2219 Size: 6568 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 2210 Size: 6568 FspI TGC'GCA Cuts at: 2030 2096 5074 2030 Size: 66 2978 3524 Fragments arranged by size: 3524 2978 66 HaeII r_GCGC'y Cuts at: 1678 3197 5715 1678 Size: 1519 2518 2531 Fragments arranged by size: 2531 2518 1519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2558 2590 3822 3854 5209 Size: 32 135 32 986 32 1232 32 1355 Cuts at: 5209 5241 5283 5315 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1355 1232 986 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3912 4158 669 Size: 988 281 1974 246 3079 Fragments arranged by size: 3079 1974 988 281 246 HindIII A'AGCT_T Cuts at: 748 3726 748 Size: 2978 3590 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 2832 2851 5682 5866 1305 Size: 1086 441 19 2831 184 2007 Fragments arranged by size: 2831 2007 1086 441 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 2663 2891 3605 3707 10 Size: 54 1425 343 831 228 714 102 2871 Fragments arranged by size: 2871 1425 831 714 343 228 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2420 3392 4526 4885 5044 518 Size: 648 1236 18 972 1134 359 159 2042 Fragments arranged by size: 2042 1236 1134 972 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2910 3586 3760 3984 4661 5809 6054 1606 Size: 1304 676 174 224 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 677 676 245 224 174 NaeI GCC'GGC Cuts at: 3337 3337 Size: 6568 NcoI C'CATG_G Cuts at: 513 1065 3144 3463 513 Size: 552 2079 319 3618 Fragments arranged by size: 3618 2079 552 319 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NgoMIV G'CCGG_C Cuts at: 3335 3335 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 3335 3867 3926 5471 1132 Size: 97 2106 532 59 1545 2229 Fragments arranged by size: 2229 2106 1545 532 97 59 PasI CC'CwG_GG Cuts at: 1348 3427 1348 Size: 2079 4489 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2558 2640 3853 1108 Size: 1450 82 1213 3823 Fragments arranged by size: 3823 1450 1213 82 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2219 2219 Size: 6568 PshAI GACnn'nnGTC Cuts at: 1613 2693 1613 Size: 1080 5488 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PstI C_TGCA'G Cuts at: 830 3201 3330 3920 830 Size: 2371 129 590 3478 Fragments arranged by size: 3478 2371 590 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 3586 3760 3586 Size: 174 6394 RsrII CG'GwC_CG Cuts at: 3534 3534 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SanDI GG'GwC_CC Cuts at: 2219 2219 Size: 6568 SapI GCTCTTCn'nnn_ Cuts at: 2027 2303 2027 Size: 276 6292 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SfcI C'TryA_G Cuts at: 826 1045 3197 3326 3916 5051 5732 5923 826 Size: 219 2152 129 590 1135 681 191 1471 Fragments arranged by size: 2152 1471 1135 681 590 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2948 3821 4672 5573 5835 Size: 197 926 510 495 873 851 901 262 Cuts at: 5835 820 Size: 1553 Fragments arranged by size: 1553 926 901 873 851 510 495 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3335 3926 1132 Size: 2203 591 3774 Fragments arranged by size: 3774 2203 591 SspI AAT'ATT Cuts at: 5 52 2668 4492 5 Size: 47 2616 1824 2081 Fragments arranged by size: 2616 2081 1824 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2137 2314 2634 2860 3144 3463 4007 Size: 552 1072 177 320 226 284 319 544 Cuts at: 4007 513 Size: 3074 Fragments arranged by size: 3074 1072 552 544 320 319 284 226 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4595 4612 4765 4950 5395 325 Size: 1255 3015 17 153 185 445 1498 Fragments arranged by size: 3015 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 3306 4814 Size: 274 80 33 51 1443 399 930 1508 Cuts at: 4814 6526 96 Size: 1712 138 Fragments arranged by size: 1712 1508 1443 930 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3537 3569 6528 6560 3537 Size: 32 2959 32 3545 Fragments arranged by size: 3545 2959 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 2845 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 3155 3743 4697 3155 Size: 588 954 5026 Fragments arranged by size: 5026 954 588 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI