(Linear) (Six-base) MAPSORT of: KIEE6427.seq Check: 6890 from: 1 to: 1863 KIEE6427.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 257 1609 1725 1863 Size: 257 1352 116 138 Fragments arranged by size: 1352 257 138 116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 549 1863 Size: 549 1314 ApaLI G'TGCA_C Cuts at: 0 1468 1863 Size: 1468 395 ApoI r'AATT_y Cuts at: 0 226 1012 1033 1863 Size: 226 786 21 830 Fragments arranged by size: 830 786 226 21 AvaI C'yCGr_G Cuts at: 0 1201 1863 Size: 1201 662 AvrII C'CTAG_G Cuts at: 0 132 309 1863 Size: 132 177 1554 Fragments arranged by size: 1554 177 132 BamHI G'GATC_C Cuts at: 0 1352 1863 Size: 1352 511 BanII G_rGCy'C Cuts at: 0 17 802 1673 1863 Size: 17 785 871 190 Fragments arranged by size: 871 785 190 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 605 1863 Size: 605 1258 BciVI GTATCCnnnnn_n' Cuts at: 0 1386 1863 Size: 1386 477 Bme1580I G_kGCm'C Cuts at: 0 1472 1842 1863 Size: 1472 370 21 Fragments arranged by size: 1472 370 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 264 1863 Size: 264 1599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1754 1863 Size: 1754 109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 928 1837 1863 Size: 433 495 909 26 Fragments arranged by size: 909 495 433 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 69 1863 Size: 69 1794 BsaBI GATnn'nnATC Cuts at: 0 1303 1863 Size: 1303 560 BsaHI Gr'CG_yC Cuts at: 0 508 1863 Size: 508 1355 BsaWI w'CCGG_w Cuts at: 0 575 1295 1863 Size: 575 720 568 Fragments arranged by size: 720 575 568 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1532 1562 1863 Size: 1532 30 301 Fragments arranged by size: 1532 301 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 490 1255 1519 1588 1863 Size: 490 765 264 69 275 Fragments arranged by size: 765 490 275 264 69 BseYI C'CCAG_C Cuts at: 0 934 1805 1863 Size: 934 871 58 Fragments arranged by size: 934 871 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 963 1863 Size: 963 900 BsiEI CG_ry'CG Cuts at: 0 909 1335 1527 1634 1863 Size: 909 426 192 107 229 Fragments arranged by size: 909 426 229 192 107 BsiHKAI G_wGCw'C Cuts at: 0 521 1384 1472 1863 Size: 521 863 88 391 Fragments arranged by size: 863 521 391 88 BsiWI C'GTAC_G Cuts at: 0 1232 1863 Size: 1232 631 BsmI GAATG_Cn' Cuts at: 0 730 791 1863 Size: 730 61 1072 Fragments arranged by size: 1072 730 61 Bsp1286I G_dGCh'C Cuts at: 0 17 521 802 1384 1472 1673 1842 1863 Size: 17 504 281 582 88 201 169 21 Fragments arranged by size: 582 504 281 201 169 88 21 17 BspEI T'CCGG_A Cuts at: 0 575 1295 1863 Size: 575 720 568 Fragments arranged by size: 720 575 568 BsrBI CCG'CTC Cuts at: 0 5 1655 1863 Size: 5 1650 208 Fragments arranged by size: 1650 208 5 BsrDI GCAATG_nn' Cuts at: 0 521 1863 Size: 521 1342 BsrFI r'CCGG_y Cuts at: 0 77 908 1330 1863 Size: 77 831 422 533 Fragments arranged by size: 831 533 422 77 BsrGI T'GTAC_A Cuts at: 0 1301 1863 Size: 1301 562 BssSI C'ACGA_G Cuts at: 0 41 1687 1863 Size: 41 1646 176 Fragments arranged by size: 1646 176 41 BstXI CCAn_nnnn'nTGG Cuts at: 0 442 1863 Size: 442 1421 BstYI r'GATC_y Cuts at: 0 1196 1352 1811 1863 Size: 1196 156 459 52 Fragments arranged by size: 1196 459 156 52 BtgI C'CryG_G Cuts at: 0 29 218 1139 1458 1532 1863 Size: 29 189 921 319 74 331 Fragments arranged by size: 921 331 319 189 74 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 575 1290 1863 Size: 575 715 573 Fragments arranged by size: 715 575 573 BtsI GCAGTG_nn' Cuts at: 0 1180 1540 1863 Size: 1180 360 323 Fragments arranged by size: 1180 360 323 DraIII CAC_nnn'GTG Cuts at: 0 595 1863 Size: 595 1268 EaeI y'GGCC_r Cuts at: 0 656 884 906 1332 1401 1524 1598 1631 Size: 656 228 22 426 69 123 74 33 Cuts at: 1631 1700 1863 Size: 69 163 Fragments arranged by size: 656 426 228 163 123 74 69 69 33 22 EagI C'GGCC_G Cuts at: 0 906 1332 1524 1631 1863 Size: 906 426 192 107 232 Fragments arranged by size: 906 426 232 192 107 EarI CTCTTCn'nnn_ Cuts at: 0 22 233 298 1744 1863 Size: 22 211 65 1446 119 Fragments arranged by size: 1446 211 119 65 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 257 1609 1725 1754 1863 Size: 257 1352 116 29 109 Fragments arranged by size: 1352 257 116 109 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 859 981 1863 Size: 859 122 882 Fragments arranged by size: 882 859 122 EcoO109I rG'GnC_Cy Cuts at: 0 214 1863 Size: 214 1649 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 205 1863 Size: 205 1658 FspI TGC'GCA Cuts at: 0 25 91 1863 Size: 25 66 1772 Fragments arranged by size: 1772 66 25 HaeII r_GCGC'y Cuts at: 0 1192 1863 Size: 1192 671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 553 585 1817 1849 1863 Size: 553 32 1232 32 14 Fragments arranged by size: 1232 553 32 32 14 HindIII A'AGCT_T Cuts at: 0 1721 1863 Size: 1721 142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 827 846 1863 Size: 386 441 19 1017 Fragments arranged by size: 1017 441 386 19 MscI TGG'CCA Cuts at: 0 658 886 1600 1702 1863 Size: 658 228 714 102 161 Fragments arranged by size: 714 658 228 161 102 MslI CAynn'nnrTG Cuts at: 0 397 415 1387 1863 Size: 397 18 972 476 Fragments arranged by size: 972 476 397 18 MspA1I CmG'CkG Cuts at: 0 905 1581 1755 1863 Size: 905 676 174 108 Fragments arranged by size: 905 676 174 108 NaeI GCC'GGC Cuts at: 0 1332 1863 Size: 1332 531 NcoI C'CATG_G Cuts at: 0 1139 1458 1863 Size: 1139 319 405 Fragments arranged by size: 1139 405 319 NgoMIV G'CCGG_C Cuts at: 0 1330 1863 Size: 1330 533 NspI r_CATG'y Cuts at: 0 1330 1862 1863 Size: 1330 532 1 Fragments arranged by size: 1330 532 1 PasI CC'CwG_GG Cuts at: 0 1422 1863 Size: 1422 441 PflMI CCAn_nnn'nTGG Cuts at: 0 553 635 1848 1863 Size: 553 82 1213 15 Fragments arranged by size: 1213 553 82 15 PpuMI rG'GwC_Cy Cuts at: 0 214 1863 Size: 214 1649 PshAI GACnn'nnGTC Cuts at: 0 688 1863 Size: 688 1175 PstI C_TGCA'G Cuts at: 0 1196 1325 1863 Size: 1196 129 538 Fragments arranged by size: 1196 538 129 PvuII CAG'CTG Cuts at: 0 1581 1755 1863 Size: 1581 174 108 Fragments arranged by size: 1581 174 108 RsrII CG'GwC_CG Cuts at: 0 1529 1863 Size: 1529 334 SanDI GG'GwC_CC Cuts at: 0 214 1863 Size: 214 1649 SapI GCTCTTCn'nnn_ Cuts at: 0 22 298 1863 Size: 22 276 1565 Fragments arranged by size: 1565 276 22 SfcI C'TryA_G Cuts at: 0 1192 1321 1863 Size: 1192 129 542 Fragments arranged by size: 1192 542 129 SmlI C'TyrA_G Cuts at: 0 448 943 1816 1863 Size: 448 495 873 47 Fragments arranged by size: 873 495 448 47 SphI G_CATG'C Cuts at: 0 1330 1863 Size: 1330 533 SspI AAT'ATT Cuts at: 0 663 1863 Size: 663 1200 StyI C'CwwG_G Cuts at: 0 132 309 629 855 1139 1458 1863 Size: 132 177 320 226 284 319 405 Fragments arranged by size: 405 320 319 284 226 177 132 TatI w'GTAC_w Cuts at: 0 371 1301 1863 Size: 371 930 562 Fragments arranged by size: 930 562 371 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1532 1564 1863 Size: 1532 32 299 Fragments arranged by size: 1532 299 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 840 1863 Size: 840 1023 XmnI GAAnn'nnTTC Cuts at: 0 1150 1738 1863 Size: 1150 588 125 Fragments arranged by size: 1150 588 125 Enzymes that do cut: AcuI AlfI ApaLI ApoI AvaI AvrII BamHI BanII BbsI BciVI Bme1580I BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpuMI PshAI PstI PvuII RsrII SanDI SapI SfcI SmlI SphI SspI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaAI BsmBI BspHI BspMI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FseI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI