(Circular) (Six-base) MAPSORT of: pFN21AB6665.seq Check: 8187 from: 1 to: 5977 pFN21AB6665 5977 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 2465 3197 4040 5424 5692 1632 Size: 824 9 732 843 1384 268 1917 Fragments arranged by size: 1917 1384 843 824 732 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2052 2632 2761 3720 5292 2052 Size: 580 129 959 1572 2737 Fragments arranged by size: 2737 1572 959 580 129 ApaI G_GGCC'C Cuts at: 2188 2219 2188 Size: 31 5946 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3028 3302 1870 Size: 73 1085 274 4545 Fragments arranged by size: 4545 1085 274 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 3005 3207 3272 3298 Size: 325 217 627 313 905 202 65 26 Cuts at: 3298 4753 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 905 627 325 313 217 202 65 26 BanII G_rGCy'C Cuts at: 1391 2188 2219 2963 3296 1391 Size: 797 31 744 333 4072 Fragments arranged by size: 4072 797 744 333 31 BbeI G_GCGC'C Cuts at: 3276 3276 Size: 5977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2504 2612 928 Size: 971 605 108 4293 Fragments arranged by size: 4293 971 605 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2597 3237 4588 Size: 107 122 71 631 605 925 640 1351 Cuts at: 4588 136 Size: 1525 Fragments arranged by size: 1525 1351 925 640 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2268 2276 3149 3394 2268 Size: 8 873 245 4851 Fragments arranged by size: 4851 873 245 8 Bme1580I G_kGCm'C Cuts at: 1790 2103 2188 2219 3115 4038 5194 1790 Size: 313 85 31 896 923 1156 2573 Fragments arranged by size: 2573 1156 923 896 313 85 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2215 3016 Size: 416 53 83 399 24 774 24 801 Cuts at: 3016 3708 4666 442 Size: 692 958 1753 Fragments arranged by size: 1753 958 801 774 692 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1995 2046 2255 2312 4637 1239 Size: 756 51 209 57 2325 2579 Fragments arranged by size: 2579 2325 756 209 57 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2684 4640 882 Size: 617 1185 1956 2219 Fragments arranged by size: 2219 1956 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 3259 3768 493 Size: 711 2055 509 2702 Fragments arranged by size: 2702 2055 711 509 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3273 3784 4166 4801 Size: 53 83 186 936 1740 511 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1740 1451 936 635 511 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 3071 3182 3203 1186 Size: 1732 153 111 21 3960 Fragments arranged by size: 3960 1732 153 111 21 BseYI C'CCAG_C Cuts at: 2166 2371 5180 5676 2166 Size: 205 2809 496 2467 Fragments arranged by size: 2809 2467 496 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 1163 Size: 573 534 4870 Fragments arranged by size: 4870 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2400 3427 4188 5216 1540 Size: 275 186 399 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 399 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2548 2548 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2188 2219 2963 3115 3296 4038 Size: 399 313 85 31 744 152 181 742 Cuts at: 4038 4123 5194 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 744 742 399 313 181 152 85 85 31 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 3332 844 Size: 1858 630 3489 Fragments arranged by size: 3489 1858 630 BsrBI CCG'CTC Cuts at: 1394 1823 2272 2329 3864 1394 Size: 429 449 57 1535 3507 Fragments arranged by size: 3507 1535 449 429 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 2361 4621 4808 1700 Size: 110 293 258 2260 187 2869 Fragments arranged by size: 2869 2260 293 258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 2140 2280 2335 1925 Size: 215 140 55 5567 Fragments arranged by size: 5567 215 140 55 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 2971 1184 Size: 1787 4190 BstXI CCAn_nnnn'nTGG Cuts at: 3259 3259 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2842 3307 3674 4059 4076 5528 5614 5626 Size: 870 465 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 870 465 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2038 2038 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2866 513 Size: 552 939 201 661 3624 Fragments arranged by size: 3624 939 661 552 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2630 487 Size: 683 56 1404 3834 Fragments arranged by size: 3834 1404 683 56 BtsI GCAGTG_nn' Cuts at: 2782 3493 4287 4307 2782 Size: 711 794 20 4452 Fragments arranged by size: 4452 794 711 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 4984 809 Size: 507 1224 2444 1802 Fragments arranged by size: 2444 1802 1224 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2880 2891 Size: 54 1425 50 275 18 567 483 11 Cuts at: 2891 2953 3130 3229 3424 4313 5942 8 Size: 62 177 99 195 889 1629 43 Fragments arranged by size: 1629 1425 889 567 483 275 195 177 99 62 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2397 3424 1537 Size: 275 585 1027 4090 Fragments arranged by size: 4090 1027 585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2432 2832 3066 3907 1353 Size: 657 422 400 234 841 3423 Fragments arranged by size: 3423 841 657 422 400 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2046 2255 2312 2456 2465 3197 Size: 393 363 51 209 57 144 9 732 Cuts at: 3197 4040 4637 5424 5692 1239 Size: 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 732 597 393 363 268 209 144 57 51 9 EcoO109I rG'GnC_Cy Cuts at: 2116 2215 2216 3033 2116 Size: 99 1 817 5060 Fragments arranged by size: 5060 817 99 1 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 2561 4483 2561 Size: 1922 4055 HaeII r_GCGC'y Cuts at: 1678 2541 2713 3276 5124 1678 Size: 863 172 563 1848 2531 Fragments arranged by size: 2531 1848 863 563 172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 2894 2926 3047 Size: 32 135 32 1027 32 263 32 121 Cuts at: 3047 3079 4618 4650 4692 4724 1373 Size: 32 1539 32 42 32 2626 Fragments arranged by size: 2626 1539 1027 263 135 121 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KasI G'GCGC_C Cuts at: 3272 3272 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 2893 3132 10 Size: 54 1425 343 1050 11 239 2855 Fragments arranged by size: 2855 1425 1050 343 239 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2882 3935 4294 4453 518 Size: 648 1716 1053 359 159 2042 Fragments arranged by size: 2042 1716 1053 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 2127 2347 2557 2716 3393 4070 5218 Size: 480 41 220 210 159 677 677 1148 Cuts at: 5218 5463 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 677 480 245 220 210 159 41 NarI GG'CG_CC Cuts at: 3273 3273 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2252 3335 4880 1132 Size: 97 1023 1083 1545 2229 Fragments arranged by size: 2229 1545 1083 1023 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 2761 2889 1108 Size: 1065 588 128 4196 Fragments arranged by size: 4196 1065 588 128 PfoI T'CCnGG_A Cuts at: 2133 2133 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 3033 2116 Size: 917 5060 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 2184 2215 2184 Size: 31 5946 PstI C_TGCA'G Cuts at: 830 2571 3329 830 Size: 1741 758 3478 Fragments arranged by size: 3478 1741 758 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2127 2557 2127 Size: 430 5547 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 3033 3033 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SexAI A'CCwGG_T Cuts at: 2118 2118 Size: 5977 SfcI C'TryA_G Cuts at: 826 1045 2068 2567 3325 4460 5141 5332 826 Size: 219 1023 499 758 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 758 681 499 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3237 3237 Size: 5977 SfoI GGC'GCC Cuts at: 3274 3274 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3030 3304 1872 Size: 1158 274 4545 Fragments arranged by size: 4545 1158 274 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 2252 3335 1132 Size: 1120 1083 3774 Fragments arranged by size: 3774 1120 1083 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2036 2243 2684 1517 Size: 519 207 441 4810 Fragments arranged by size: 4810 519 441 207 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 2913 3211 3416 513 Size: 552 939 588 321 298 205 3074 Fragments arranged by size: 3074 939 588 552 321 298 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2879 4004 4021 4174 4359 4804 325 Size: 1255 1299 1125 17 153 185 445 1498 Fragments arranged by size: 1498 1299 1255 1125 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3044 3076 5937 5969 3044 Size: 32 2861 32 3052 Fragments arranged by size: 3052 2861 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2120 1316 Size: 804 5173 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3224 3224 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3028 3302 1870 Size: 1158 274 4545 Fragments arranged by size: 4545 1158 274 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SrfI SwaI