################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanII BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KpnI MluI MluNI MroI MscI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SalI SbfI ScaI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SwaI TaqI TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 358 GTAGAC GT^[AC][GT]AC AlwI 705 GGATC GGATC(4/5) AocI 1179 CCTGAGG CC^T[ACGT]AGG AspI 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 292 CCTCAGC CCTCAGC(-5/-2) BcnI 139 CCCGG CC^[GC]GG BfaI 709 CTAG C^TAG BglII 645 AGATCT A^GATCT BlpI 15 GCTGAGC GC^T[ACGT]AGC BmyI 43 GTGCCC G[AGT]GC[ACT]^C Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC BsaBI 646 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 462 GAGACC GGTCTC(1/5) BsiEI 1246 CGATCG CG[GA][CT]^CG Bsp1286I 43 GTGCCC G[AGT]GC[ACT]^C BsrDI 742 GCAATG GCAATG(2/0) BstEII 386 GGTTACC G^GT[ACGT]ACC BstPI 386 GGTTACC G^GT[ACGT]ACC Bsu36I 1179 CCTGAGG CC^T[ACGT]AGG CelII 15 GCTGAGC GC^T[ACGT]AGC DraI 1167 TTTAAA TTT^AAA Eco81I 1179 CCTGAGG CC^T[ACGT]AGG EcoO65I 386 GGTTACC G^GT[ACGT]ACC EcoT14I 776 CCATGG C^C[AT][AT]GG HincII 1045 GTTAAC GT[CT]^[GA]AC HindII 1045 GTTAAC GT[CT]^[GA]AC HpaI 1045 GTTAAC GTT^AAC KspI 232 CCGCGG CCGC^GG MaeI 709 CTAG C^TAG MaeII 410 ACGT A^CGT MamI 646 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 992 CAATTG C^AATTG MunI 992 CAATTG C^AATTG NciI 139 CCCGG CC^[GC]GG NcoI 776 CCATGG C^CATGG NdeI 1351 CATATG CA^TATG NspI 839 GCATGC [GA]CATG^[CT] PflFI 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1185 GACTC GAGTC(4/5) PstI 818 CTGCAG CTGCA^G PvuI 1246 CGATCG CGAT^CG PvuII 770 CAGCTG CAG^CTG SacII 232 CCGCGG CCGC^GG SapI 132 GCTCTTC GCTCTTC(1/4) SexAI 251 ACCTGGT A^CC[AT]GGT SfcI 818 CTGCAG C^T[GA][CT]AG SgrAI 53 CGCCGGCG C[GA]^CCGG[CT]G SmlI 88 CTCAAG C^T[CT][GA]AG SphI 839 GCATGC GCATG^C SspI 613 AATATT AAT^ATT StuI 783 AGGCCT AGG^CCT StyI 776 CCATGG C^C[AT][AT]GG TfiI 111 GAATC G^A[AT]TC Tsp45I 906 GTCAC ^GT[GC]AC Tth111I 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 708 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 960 GAATTT [GA]^AATT[CT] 1023 AAATTT AfaI 684 GTAC GT^AC 1214 GTAC ApoI 960 GAATTT [GA]^AATT[CT] 1023 AAATTT Asp700I 408 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 825 GAAACAATTC BbsI 412 GTCTTC GAAGAC(2/6) 1158 GTCTTC BpuAI 412 GTCTTC GAAGAC(2/6) 1158 GTCTTC BsaWI 702 ACCGGA [AT]^CCGG[AT] 1271 ACCGGA BseRI 533 CTCCTC GAGGAG(10/8) 145 GAGGAG BsmAI 341 GAGAC GTCTC(1/5) 462 GAGAC BsmFI 36 GGGAC GGGAC(10/14) 661 GTCCC BstYI 645 AGATCT [GA]^GATC[CT] 705 GGATCT DsaI 232 CCGCGG C^C[GA][CT]GG 776 CCATGG EarI 133 CTCTTC CTCTTC(1/4) 1356 GAAGAG HgaI 124 GACGC GACGC(5/10) 1109 GACGC HinfI 111 GAATC G^A[ACGT]TC 1185 GACTC Ksp632I 133 CTCTTC CTCTTC(1/4) 1356 GAAGAG MflI 645 AGATCT [GA]^GATC[CT] 705 GGATCT MspA1I 232 CCGCGG C[AC]G^C[GT]G 770 CAGCTG NaeI 54 GCCGGC GCC^GGC 207 GCCGGC NgoMIV 54 GCCGGC G^CCGGC 207 GCCGGC PpuMI 36 GGGACCC [GA]G^G[AT]CC[CT] 248 AGGACCT RsaI 684 GTAC GT^AC 1214 GTAC SfaNI 951 GATGC GCATC(5/9) 1112 GCATC XhoII 645 AGATCT [GA]^GATC[CT] 705 GGATCT XmnI 408 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 825 GAAACAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BanI BbeI BbvI BpmI BsaHI BsaJI BsiYI BslI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NarI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfoI Tru9I TseI Tsp509I TspRI