(Circular) (Six-base) MAPSORT of: pFN21AB7783.seq Check: 5923 from: 1 to: 6076 pFN21AB7783 6076 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2364 3419 1937 Size: 427 1055 4594 Fragments arranged by size: 4594 1055 427 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 3375 4203 4576 1563 Size: 1812 828 373 3063 Fragments arranged by size: 3063 1812 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2406 4139 5523 5791 1632 Size: 545 229 1733 1384 268 1917 Fragments arranged by size: 1917 1733 1384 545 268 229 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 4975 5952 1203 Size: 3772 977 1327 Fragments arranged by size: 3772 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 2520 3198 1166 Size: 1354 678 4044 Fragments arranged by size: 4044 1354 678 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6076 AlwNI CAG_nnn'CTG Cuts at: 2498 2698 2930 3819 5391 2498 Size: 200 232 889 1572 3183 Fragments arranged by size: 3183 1572 889 232 200 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 2965 3028 3385 3611 3763 5872 1493 Size: 1472 63 357 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 357 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 3401 1870 Size: 73 1458 4545 Fragments arranged by size: 4545 1458 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3202 3235 3202 Size: 33 6043 BamHI G'GATC_C Cuts at: 3406 3773 3406 Size: 367 5709 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2108 3290 3366 3397 Size: 325 217 627 275 46 1182 76 31 Cuts at: 3397 4852 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1182 627 325 275 217 76 46 31 BanII G_rGCy'C Cuts at: 1391 3395 1391 Size: 2004 4072 BbeI G_GCGC'C Cuts at: 2066 2112 3294 2066 Size: 46 1182 4848 Fragments arranged by size: 4848 1182 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 3156 928 Size: 971 511 746 3848 Fragments arranged by size: 3848 971 746 511 BbvCI CC'TCA_GC Cuts at: 2298 2298 Size: 6076 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4267 4301 1903 Size: 34 2330 34 3678 Fragments arranged by size: 3678 2330 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 5178 3968 Size: 1210 4866 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4687 136 Size: 107 122 71 631 605 3015 1525 Fragments arranged by size: 3015 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2650 6071 2650 Size: 3421 2655 BlpI GC'TnA_GC Cuts at: 2021 3493 2021 Size: 1472 4604 Bme1580I G_kGCm'C Cuts at: 1790 2052 4137 5293 1790 Size: 262 2085 1156 2573 Fragments arranged by size: 2573 2085 1156 262 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2718 3807 4765 Size: 416 53 83 399 24 1301 1089 958 Cuts at: 4765 442 Size: 1753 Fragments arranged by size: 1753 1301 1089 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2292 2589 2945 3259 4736 1239 Size: 993 60 297 356 314 1477 2579 Fragments arranged by size: 2579 1477 993 356 314 297 60 Bpu10I CC'TnA_GC Cuts at: 2298 2298 Size: 6076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2078 4201 5066 5364 2078 Size: 2123 865 298 2790 Fragments arranged by size: 2790 2123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2461 4739 882 Size: 617 962 2278 2219 Fragments arranged by size: 2278 2219 962 617 BsaAI yAC'GTr Cuts at: 493 1204 3867 493 Size: 711 2663 2702 Fragments arranged by size: 2702 2663 711 BsaBI GATnn'nnATC Cuts at: 2655 6070 2655 Size: 3415 2661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 2109 3291 3883 Size: 53 83 186 936 530 46 1182 592 Cuts at: 3883 4265 4900 275 Size: 382 635 1451 Fragments arranged by size: 1451 1182 936 635 592 530 382 186 83 53 46 BsaWI w'CCGG_w Cuts at: 2707 3276 4509 4907 5181 5328 5739 2707 Size: 569 1233 398 274 147 411 3044 Fragments arranged by size: 3044 1233 569 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2521 2551 2587 2617 2962 Size: 30 165 30 1118 30 36 30 345 Cuts at: 2962 2992 1178 Size: 30 4262 Fragments arranged by size: 4262 1118 345 165 36 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2529 1186 Size: 979 364 4733 Fragments arranged by size: 4733 979 364 BseYI C'CCAG_C Cuts at: 2052 2104 2217 2673 5279 5775 2052 Size: 52 113 456 2606 496 2353 Fragments arranged by size: 2606 2353 496 456 113 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5503 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3254 3526 4287 5315 1540 Size: 275 186 1253 272 761 1028 2301 Fragments arranged by size: 2301 1253 1028 761 275 272 186 BsiHKAI G_wGCw'C Cuts at: 4137 4222 5293 4137 Size: 85 1071 4920 Fragments arranged by size: 4920 1071 85 BsmI GAATG_Cn' Cuts at: 3587 3680 3587 Size: 93 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 3395 4137 4222 5293 1391 Size: 399 262 1343 742 85 1071 2174 Fragments arranged by size: 2174 1343 1071 742 399 262 85 BspEI T'CCGG_A Cuts at: 5739 5739 Size: 6076 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 844 Size: 2587 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3963 1394 Size: 429 2140 3507 Fragments arranged by size: 3507 2140 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2754 4571 4745 65 Size: 1183 1506 1817 174 1396 Fragments arranged by size: 1817 1506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2077 2098 2212 2231 4720 4907 Size: 110 249 18 21 114 19 2489 187 Cuts at: 4907 1700 Size: 2869 Fragments arranged by size: 2869 2489 249 187 114 110 21 19 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 2391 1184 Size: 1207 4869 BstYI r'GATC_y Cuts at: 1972 2650 2710 3406 3773 4158 4175 5627 5713 Size: 678 60 696 367 385 17 1452 86 Cuts at: 5713 5725 6071 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 696 678 385 367 346 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 3185 3185 Size: 6076 BtgI C'CryG_G Cuts at: 513 1065 2004 2237 2781 513 Size: 552 939 233 544 3808 Fragments arranged by size: 3808 939 552 544 233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 2832 3592 4386 4406 2832 Size: 760 794 20 4502 Fragments arranged by size: 4502 794 760 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3174 3380 3174 Size: 206 5870 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3523 4412 6041 Size: 54 1425 50 275 18 1693 889 1629 Cuts at: 6041 8 Size: 43 Fragments arranged by size: 1693 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3356 4006 1353 Size: 791 1212 650 3423 Fragments arranged by size: 3423 1212 791 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4663 5037 5183 387 Size: 1064 138 3074 374 146 1280 Fragments arranged by size: 3074 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2232 2292 2406 2589 2945 3259 Size: 393 545 55 60 114 183 356 314 Cuts at: 3259 4139 4736 5523 5791 1239 Size: 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 597 545 393 356 314 268 183 114 60 55 EcoO109I rG'GnC_Cy Cuts at: 2042 2203 2254 2557 2042 Size: 161 51 303 5561 Fragments arranged by size: 5561 303 161 51 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2034 2066 2112 2138 3294 5223 1678 Size: 356 32 46 26 1156 1929 2531 Fragments arranged by size: 2531 1929 1156 356 46 32 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2521 2553 2587 Size: 32 135 32 767 32 150 32 34 Cuts at: 2587 2619 4717 4749 4791 4823 1373 Size: 32 2098 32 42 32 2626 Fragments arranged by size: 2626 2098 767 150 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3052 3420 3666 669 Size: 988 281 1114 368 246 3079 Fragments arranged by size: 3079 1114 988 368 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6076 HpaI GTT'AAC Cuts at: 3052 3666 3052 Size: 614 5462 KasI G'GCGC_C Cuts at: 2062 2108 3290 2062 Size: 46 1182 4848 Fragments arranged by size: 4848 1182 46 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 2997 3675 2997 Size: 678 5398 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3091 5190 5374 1305 Size: 1786 2099 184 2007 Fragments arranged by size: 2099 2007 1786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4254 Fragments arranged by size: 4254 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 3198 3214 4034 4393 4552 518 Size: 648 1354 678 16 820 359 159 2042 Fragments arranged by size: 2042 1354 820 678 648 359 159 16 MspA1I CmG'CkG Cuts at: 1606 2239 2777 3492 4169 5317 5562 1606 Size: 633 538 715 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 633 538 245 NaeI GCC'GGC Cuts at: 2061 2214 2061 Size: 153 5923 NarI GG'CG_CC Cuts at: 2063 2109 3291 2063 Size: 46 1182 4848 Fragments arranged by size: 4848 1182 46 NcoI C'CATG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3808 Fragments arranged by size: 3808 939 777 552 NdeI CA'TA_TG Cuts at: 387 3357 387 Size: 2970 3106 NgoMIV G'CCGG_C Cuts at: 2059 2212 2059 Size: 153 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NspI r_CATG'y Cuts at: 1132 1229 2848 3434 4979 1132 Size: 97 1619 586 1545 2229 Fragments arranged by size: 2229 1619 1545 586 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6076 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2066 2098 4145 4177 5658 5690 2066 Size: 32 2047 32 1481 32 2452 Fragments arranged by size: 2452 2047 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 2254 2042 Size: 212 5864 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PstI C_TGCA'G Cuts at: 830 2827 3428 830 Size: 1997 601 3478 Fragments arranged by size: 3478 1997 601 PvuI CG_AT'CG Cuts at: 2001 3254 2001 Size: 1253 4823 PvuII CAG'CTG Cuts at: 2777 2777 Size: 6076 SacII CC_GC'GG Cuts at: 2240 2240 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SanDI GG'GwC_CC Cuts at: 2042 2042 Size: 6076 SapI GCTCTTCn'nnn_ Cuts at: 2144 2144 Size: 6076 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 4324 6036 4324 Size: 1712 4364 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 6076 SfcI C'TryA_G Cuts at: 826 1045 2823 3424 4559 5240 5431 826 Size: 219 1778 601 1135 681 191 1471 Fragments arranged by size: 1778 1471 1135 681 601 219 191 SfoI GGC'GCC Cuts at: 2064 2110 3292 2064 Size: 46 1182 4848 Fragments arranged by size: 4848 1182 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 2059 1700 Size: 359 5717 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 4180 5081 5343 820 Size: 197 926 150 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 150 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 2848 3434 1132 Size: 1716 586 3774 Fragments arranged by size: 3774 1716 586 SspI AAT'ATT Cuts at: 5 52 2620 4000 5 Size: 47 2568 1380 2081 Fragments arranged by size: 2568 2081 1380 47 StuI AGG'CCT Cuts at: 1517 2790 1517 Size: 1273 4803 StyI C'CwwG_G Cuts at: 513 1065 2004 2781 3515 513 Size: 552 939 777 734 3074 Fragments arranged by size: 3074 939 777 734 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4103 4120 4273 4458 4903 325 Size: 1255 2523 17 153 185 445 1498 Fragments arranged by size: 2523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4322 6034 96 Size: 274 80 33 51 1443 2345 1712 138 Fragments arranged by size: 2345 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2887 2919 3048 3080 6036 6068 2887 Size: 32 129 32 2956 32 2895 Fragments arranged by size: 2956 2895 129 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2258 1316 Size: 942 5134 XbaI T'CTAG_A Cuts at: 2713 3412 2713 Size: 699 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 2417 2834 4205 2417 Size: 417 1371 4288 Fragments arranged by size: 4288 1371 417 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SfiI SrfI SwaI XcmI