(Circular) (Six-base) MAPSORT of: pFN21AE4411.seq Check: 8589 from: 1 to: 5977 pFN21AE4411 5977 bp With 182 enzymes: SgfI * March 28, 2012 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2568 3045 3320 1937 Size: 631 477 275 4594 Fragments arranged by size: 4594 631 477 275 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2643 4040 5424 5692 1632 Size: 710 301 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 710 301 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2846 4876 5853 1203 Size: 1643 2030 977 1327 Fragments arranged by size: 2030 1643 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 3720 5292 3720 Size: 1572 4405 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2853 2882 3286 3512 3664 5773 1493 Size: 1360 29 404 226 152 2109 1697 Fragments arranged by size: 2109 1697 1360 404 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3302 1870 Size: 73 889 470 4545 Fragments arranged by size: 4545 889 470 73 AvrII C'CTAG_G Cuts at: 2578 2578 Size: 5977 BamHI G'GATC_C Cuts at: 3205 3307 3674 3205 Size: 102 367 5508 Fragments arranged by size: 5508 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2779 3298 4753 618 Size: 325 217 627 992 519 1455 1842 Fragments arranged by size: 1842 1455 992 627 519 325 217 BanII G_rGCy'C Cuts at: 1391 2056 3296 1391 Size: 665 1240 4072 Fragments arranged by size: 4072 1240 665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 2097 928 Size: 971 147 51 4808 Fragments arranged by size: 4808 971 147 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2583 2788 4588 Size: 107 122 71 631 605 911 205 1800 Cuts at: 4588 136 Size: 1525 Fragments arranged by size: 1800 1525 911 631 605 205 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2688 3394 2688 Size: 706 5271 Bme1580I G_kGCm'C Cuts at: 1790 2798 4038 5194 1790 Size: 1008 1240 1156 2573 Fragments arranged by size: 2573 1240 1156 1008 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3005 3708 4666 Size: 416 53 83 399 24 1588 703 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1588 958 703 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2629 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2742 2792 3022 4637 1239 Size: 903 600 50 230 1615 2579 Fragments arranged by size: 2579 1615 903 600 230 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3235 3768 493 Size: 711 2031 533 2702 Fragments arranged by size: 2702 2031 711 533 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2511 4410 4808 5082 5229 5640 2511 Size: 1899 398 274 147 411 2848 Fragments arranged by size: 2848 1899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2790 2820 1178 Size: 30 165 30 1387 30 4335 Fragments arranged by size: 4335 1387 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 3173 1186 Size: 1330 657 3990 Fragments arranged by size: 3990 1330 657 BseYI C'CCAG_C Cuts at: 2061 2065 2921 5180 5676 2061 Size: 4 856 2259 496 2362 Fragments arranged by size: 2362 2259 856 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2728 4038 4123 5194 2728 Size: 1310 85 1071 3511 Fragments arranged by size: 3511 1310 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2728 2798 3296 4038 4123 5194 Size: 399 266 672 70 498 742 85 1071 Cuts at: 5194 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 672 498 399 266 85 70 BspEI T'CCGG_A Cuts at: 2511 5640 2511 Size: 3129 2848 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2118 3864 1394 Size: 429 295 1746 3507 Fragments arranged by size: 3507 1746 429 295 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 2548 96 Size: 2452 3525 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 3125 3125 Size: 5977 BstYI r'GATC_y Cuts at: 1972 3205 3307 3674 4059 4076 5528 5614 5626 Size: 1233 102 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2014 3148 513 Size: 552 949 1134 3342 Fragments arranged by size: 3342 1134 949 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2826 3058 3261 3493 4287 4307 2826 Size: 232 203 232 794 20 4496 Fragments arranged by size: 4496 794 232 232 203 20 ClaI AT'CG_AT Cuts at: 1554 2757 1554 Size: 1203 4774 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3136 3281 3136 Size: 145 5832 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 3424 4313 Size: 54 1425 50 275 18 582 1012 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1012 889 582 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 3907 1353 Size: 957 1597 3423 Fragments arranged by size: 3423 1597 957 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2342 2643 2742 2792 3022 4040 Size: 393 510 200 301 99 50 230 1018 Cuts at: 4040 4637 5424 5692 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1018 787 597 510 393 301 268 230 200 99 50 EcoNI CCTnn'n_nnAGG Cuts at: 2577 2969 2577 Size: 392 5585 EcoO109I rG'GnC_Cy Cuts at: 2709 2983 2709 Size: 274 5703 EcoRI G'AATT_C Cuts at: 2853 3286 3664 2853 Size: 433 378 5166 Fragments arranged by size: 5166 433 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2632 2975 2632 Size: 343 5634 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2231 2263 2995 3027 4618 Size: 32 135 32 659 32 732 32 1591 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1591 732 659 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3046 3321 3567 669 Size: 988 281 1108 275 246 3079 Fragments arranged by size: 3079 1108 988 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 2471 3576 2471 Size: 1105 4872 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 10 Size: 54 1425 343 582 3573 Fragments arranged by size: 3573 1425 582 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2019 3935 4294 4453 518 Size: 648 853 1916 359 159 2042 Fragments arranged by size: 2042 1916 853 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 3393 4070 5218 5463 1606 Size: 459 1328 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 459 245 NcoI C'CATG_G Cuts at: 513 1065 3148 513 Size: 552 2083 3342 Fragments arranged by size: 3342 2083 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2180 2850 3335 4880 1132 Size: 97 951 670 485 1545 2229 Fragments arranged by size: 2229 1545 951 670 485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 2846 4876 2846 Size: 2030 3947 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4309 PfoI T'CCnGG_A Cuts at: 3117 3117 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 3235 3235 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2949 2981 4046 4078 5559 5591 2949 Size: 32 1065 32 1481 32 3335 Fragments arranged by size: 3335 1481 1065 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2709 2709 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 3230 3329 830 Size: 2400 99 3478 Fragments arranged by size: 3478 2400 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2065 2065 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3044 3319 1936 Size: 1108 275 4594 Fragments arranged by size: 4594 1108 275 SapI GCTCTTCn'nnn_ Cuts at: 2310 2310 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 3220 3226 3325 4460 5141 5332 826 Size: 219 2175 6 99 1135 681 191 1471 Fragments arranged by size: 2175 1471 1135 681 219 191 99 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2583 2583 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 2834 3304 1872 Size: 962 470 4545 Fragments arranged by size: 4545 962 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2578 3148 3416 513 Size: 552 1513 570 268 3074 Fragments arranged by size: 3074 1513 570 552 268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2548 4223 5935 Size: 274 80 33 51 1443 571 1675 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1712 1675 1443 571 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2790 2822 5937 5969 2790 Size: 32 3115 32 2798 Fragments arranged by size: 3115 2798 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 2832 3302 1870 Size: 962 470 4545 Fragments arranged by size: 4545 962 470 XmnI GAAnn'nnTTC Cuts at: 2094 2745 4106 2094 Size: 651 1361 3965 Fragments arranged by size: 3965 1361 651 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI