Restriction map for
pFN21AE4338
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 341 AAATTC [GA]^AATT[CT]
AhdI 371 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 891 GATCC GGATC(4/5)
AlwNI 955 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 460 GGGCCC GGGCC^C
ApoI 341 AAATTC [GA]^AATT[CT]
AspEI 371 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 536 CCCGGG C^[CT]CG[GA]G
AviII 194 TGCGCA TGC^GCA
BalI 593 TGGCCA TGG^CCA
BanII 460 GGGCCC G[GA]GC[CT]^C
BfaI 766 CTAG C^TAG
BsmBI 410 GAGACG CGTCTC(1/5)
BsoBI 536 CCCGGG C^[CT]CG[GA]G
BspMI 499 ACCTGC ACCTGC(4/8)
BsrBI 68 CCGCTC CCGCTC(-3/-3)
BsrDI 608 GCAATG GCAATG(2/0)
BsrFI 737 GCCGGC [GA]^CCGG[CT]
BssSI 433 CACGAG CACGAG(-5/-1)
BstYI 890 AGATCC [GA]^GATC[CT]
Cfr10I 737 GCCGGC [GA]^CCGG[CT]
DdeI 112 CTCAG C^T[ACGT]AG
DpnII 891 GATC ^GATC
EaeI 593 TGGCCA [CT]^GGCC[GA]
Eam1105I 371 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 782 CCTTGG C^C[AT][AT]GG
FspI 194 TGCGCA TGC^GCA
HaeII 996 GGCGCT [GA]GCGC^[CT]
HphI 755 TCACC GGTGA(8/7)
MaeI 766 CTAG C^TAG
MboI 891 GATC ^GATC
MflI 890 AGATCC [GA]^GATC[CT]
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
NaeI 737 GCCGGC GCC^GGC
NdeII 891 GATC ^GATC
NgoMIV 737 GCCGGC G^CCGGC
NspI 467 GCATGT [GA]CATG^[CT]
PvuII 562 CAGCTG CAG^CTG
Sau3AI 891 GATC ^GATC
SmaI 536 CCCGGG CCC^GGG
StuI 672 AGGCCT AGG^CCT
StyI 782 CCTTGG C^C[AT][AT]GG
TaqI 446 TCGA T^CGA
TthHB8I 446 TCGA T^CGA
XcmI 734 CCAGCCGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 890 AGATCC [GA]^GATC[CT]
XmaCI 536 CCCGGG C^CCGGG
XmaI 536 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 471 GTAC GT^AC
495 GTAC
Alw44I 331 GTGCAC G^TGCAC
690 GTGCAC
ApaLI 331 GTGCAC G^TGCAC
690 GTGCAC
AspHI 331 GTGCAC G[AT]GC[AT]^C
690 GTGCAC
BsaI 289 GAGACC GGTCTC(1/5)
557 GAGACC
BsiHKAI 331 GTGCAC G[AT]GC[AT]^C
690 GTGCAC
BstAPI 186 GCACAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
467 GCATGTACTGC
BstF5I 324 CATCC GGATG(2/0)
524 GGATG
DraII 908 AGGTCCC [GA]G^G[ACGT]CC[CT]
965 AGGACCT
DsaI 53 CCACGG C^C[GA][CT]GG
704 CCGTGG
EcoO109I 908 AGGTCCC [GA]G^G[ACGT]CC[CT]
965 AGGACCT
FokI 324 CATCC GGATG(9/13)
524 GGATG
PflMI 831 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
954 CCAGGAGCTGG
PpuMI 908 AGGTCCC [GA]G^G[AT]CC[CT]
965 AGGACCT
RsaI 471 GTAC GT^AC
495 GTAC
Tsp509I 191 AATT ^AATT
342 AATT
Van91I 831 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
954 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EarI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI
Tsp45I TspRI