(Circular) (Six-base) MAPSORT of: pFN21AE4338.seq Check: 6611 from: 1 to: 5755 pFN21AE4338 5755 bp With 182 enzymes: SgfI * March 1, 2012 16:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2513 2513 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3012 3818 5202 5470 1632 Size: 1380 806 1384 268 1917 Fragments arranged by size: 1917 1384 1380 806 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 2381 4484 2381 Size: 2103 3652 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4513 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2720 1184 Size: 1536 4219 AlwNI CAG_nnn'CTG Cuts at: 2965 3498 5070 2965 Size: 533 1572 3650 Fragments arranged by size: 3650 1572 533 ApaI G_GGCC'C Cuts at: 2469 2469 Size: 5755 ApaLI G'TGCA_C Cuts at: 2336 2695 3812 4968 2336 Size: 359 1117 1156 3123 Fragments arranged by size: 3123 1156 1117 359 ApoI r'AATT_y Cuts at: 1493 2346 3064 3290 3442 5551 1493 Size: 853 718 226 152 2109 1697 Fragments arranged by size: 2109 1697 853 718 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2541 3080 1870 Size: 73 598 539 4545 Fragments arranged by size: 4545 598 539 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 4531 618 Size: 325 217 627 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 325 217 BanII G_rGCy'C Cuts at: 1391 2469 3074 1391 Size: 1078 605 4072 Fragments arranged by size: 4072 1078 605 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3946 3980 1903 Size: 34 50 34 1925 34 3678 Fragments arranged by size: 3678 1925 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2340 2469 2699 3816 4972 1790 Size: 550 129 230 1117 1156 2573 Fragments arranged by size: 2573 1156 1117 550 230 129 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 2987 2996 4415 1239 Size: 840 908 9 1419 2579 Fragments arranged by size: 2579 1419 908 840 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2288 2556 4418 882 Size: 617 789 268 1862 2219 Fragments arranged by size: 2219 1862 789 617 268 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2077 2107 1178 Size: 30 165 30 674 30 4826 Fragments arranged by size: 4826 674 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2807 2963 2995 1186 Size: 961 660 156 32 3946 Fragments arranged by size: 3946 961 660 156 32 BseYI C'CCAG_C Cuts at: 2279 2468 3004 3037 4958 5454 2279 Size: 189 536 33 1921 496 2580 Fragments arranged by size: 2580 1921 536 496 189 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2521 2572 3015 1163 Size: 573 785 51 443 3903 Fragments arranged by size: 3903 785 573 443 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2699 3816 3901 4972 2340 Size: 359 1117 85 1071 3123 Fragments arranged by size: 3123 1117 1071 359 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 BsmBI CGTCTCn'nnnn_ Cuts at: 2409 2409 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2469 2699 3074 3816 3901 4972 Size: 399 550 129 230 375 742 85 1071 Cuts at: 4972 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 399 375 230 129 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2513 3110 844 Size: 1669 597 3489 Fragments arranged by size: 3489 1669 597 BsrBI CCG'CTC Cuts at: 1394 1823 2075 3642 1394 Size: 429 252 1567 3507 Fragments arranged by size: 3507 1567 429 252 BsrDI GCAATG_nn' Cuts at: 65 1248 2620 4250 4424 65 Size: 1183 1372 1630 174 1396 Fragments arranged by size: 1630 1396 1372 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2742 4399 4586 1700 Size: 110 932 1657 187 2869 Fragments arranged by size: 2869 1657 932 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 2438 3815 4827 2438 Size: 1377 1012 3366 Fragments arranged by size: 3366 1377 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2197 2478 794 Size: 1403 281 4071 Fragments arranged by size: 4071 1403 281 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2895 3085 3452 3837 3854 5306 5392 5404 Size: 923 190 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 923 385 367 346 190 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2058 2709 513 Size: 552 993 651 3559 Fragments arranged by size: 3559 993 651 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2584 3032 3271 4065 4085 2584 Size: 448 239 794 20 4254 Fragments arranged by size: 4254 794 448 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 3202 4091 Size: 54 1425 50 275 18 768 604 889 Cuts at: 4091 5720 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 768 604 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2208 2988 3042 3685 1353 Size: 855 780 54 643 3423 Fragments arranged by size: 3423 855 780 643 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2987 2996 3012 3818 4415 5202 Size: 393 447 908 9 16 806 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 908 806 787 597 447 393 268 16 9 EcoO109I rG'GnC_Cy Cuts at: 2914 2971 2914 Size: 57 5698 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 5755 FspI TGC'GCA Cuts at: 2201 4261 2201 Size: 2060 3695 HaeII r_GCGC'y Cuts at: 1678 3005 4902 1678 Size: 1327 1897 2531 Fragments arranged by size: 2531 1897 1327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2367 2399 4396 Size: 32 135 32 505 32 258 32 1997 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1997 505 258 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2102 2296 4869 5053 1305 Size: 797 194 2573 184 2007 Fragments arranged by size: 2573 2007 797 194 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 10 Size: 54 1425 343 768 3165 Fragments arranged by size: 3165 1425 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2408 2594 3713 4072 4231 518 Size: 648 1167 75 186 1119 359 159 2042 Fragments arranged by size: 2042 1167 1119 648 359 186 159 75 MspA1I CmG'CkG Cuts at: 1606 2011 2569 3029 3171 3848 4996 5241 1606 Size: 405 558 460 142 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 460 405 245 142 NaeI GCC'GGC Cuts at: 2744 2744 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2742 2742 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2476 3113 4658 1132 Size: 97 1247 637 1545 2229 Fragments arranged by size: 2229 1545 1247 637 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2842 2965 1108 Size: 1734 123 3898 Fragments arranged by size: 3898 1734 123 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2914 2971 2914 Size: 57 5698 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2465 2465 Size: 5755 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2569 2569 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 3103 4238 4919 5110 826 Size: 219 2058 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 2543 3082 1872 Size: 671 539 4545 Fragments arranged by size: 4545 671 539 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2679 1517 Size: 1162 4593 StyI C'CwwG_G Cuts at: 513 1065 2787 3194 513 Size: 552 1722 407 3074 Fragments arranged by size: 3074 1722 552 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2808 3782 3799 3952 4137 4582 325 Size: 1255 1228 974 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1228 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 2499 4001 Size: 274 80 33 51 1443 498 24 1502 Cuts at: 4001 5713 96 Size: 1712 138 Fragments arranged by size: 1712 1502 1443 498 274 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2537 2569 2611 2643 5715 5747 2537 Size: 32 42 32 3072 32 2545 Fragments arranged by size: 3072 2545 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2746 2746 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 2541 3080 1870 Size: 671 539 4545 Fragments arranged by size: 4545 671 539 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI