(Circular) (Six-base) MAPSORT of: pFN21AB6184.seq Check: 3202 from: 1 to: 6805 pFN21AB6184 6805 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3979 3979 Size: 6805 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4615 278 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4148 1937 Size: 2211 4594 Acc65I G'GTAC_C Cuts at: 4126 4126 Size: 6805 AclI AA'CG_TT Cuts at: 1563 4932 5305 1563 Size: 3369 373 3063 Fragments arranged by size: 3369 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2952 3213 3620 4088 4868 6252 6520 Size: 731 589 261 407 468 780 1384 268 Cuts at: 6520 1632 Size: 1917 Fragments arranged by size: 1917 1384 780 731 589 468 407 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6608 AflIII A'CryG_T Cuts at: 1203 5704 6681 1203 Size: 4501 977 1327 Fragments arranged by size: 4501 1327 977 AgeI A'CCGG_T Cuts at: 5636 5636 Size: 6805 AhdI GACnn_n'nnGTC Cuts at: 5534 5534 Size: 6805 AleI CACnn'nnGTG Cuts at: 1166 3434 1166 Size: 2268 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 2153 Size: 32 6773 AlwNI CAG_nnn'CTG Cuts at: 3373 3990 4057 4548 6120 3373 Size: 617 67 491 1572 4058 Fragments arranged by size: 4058 1572 617 491 67 ApaI G_GGCC'C Cuts at: 2137 2137 Size: 6805 ApaLI G'TGCA_C Cuts at: 4862 6018 4862 Size: 1156 5649 ApoI r'AATT_y Cuts at: 1493 2819 2984 4114 4340 4492 6601 1493 Size: 1326 165 1130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 1130 226 165 152 AseI AT'TA_AT Cuts at: 160 1032 5359 160 Size: 872 4327 1606 Fragments arranged by size: 4327 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 AvaI C'yCGr_G Cuts at: 1870 1943 2621 3698 3923 4130 1870 Size: 73 678 1077 225 207 4545 Fragments arranged by size: 4545 1077 678 225 207 73 BamHI G'GATC_C Cuts at: 4135 4502 4135 Size: 367 6438 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4126 5581 618 Size: 325 217 627 1310 1029 1455 1842 Fragments arranged by size: 1842 1455 1310 1029 627 325 217 BanII G_rGCy'C Cuts at: 1391 2137 2622 2772 2811 3206 3416 3737 4124 Size: 746 485 150 39 395 210 321 387 Cuts at: 4124 1391 Size: 4072 Fragments arranged by size: 4072 746 485 395 387 321 210 150 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 2666 3888 4001 928 Size: 971 381 386 1222 113 3732 Fragments arranged by size: 3732 1222 971 386 381 113 BbvCI CC'TCA_GC Cuts at: 2129 2129 Size: 6805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3178 3212 4996 5030 1903 Size: 34 1241 34 1784 34 3678 Fragments arranged by size: 3678 1784 1241 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4697 5907 4697 Size: 1210 5595 BclI T'GATC_A Cuts at: 1548 1548 Size: 6805 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3126 5416 136 Size: 107 122 71 631 605 1454 2290 1525 Fragments arranged by size: 2290 1525 1454 631 605 122 107 71 BglII A'GATC_T Cuts at: 2782 6800 2782 Size: 4018 2787 BlpI GC'TnA_GC Cuts at: 4222 4222 Size: 6805 Bme1580I G_kGCm'C Cuts at: 1790 2137 2650 3102 4866 6022 1790 Size: 347 513 452 1764 1156 2573 Fragments arranged by size: 2573 1764 1156 513 452 347 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6805 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2594 3096 3361 Size: 416 53 83 399 24 1177 502 265 Cuts at: 3361 3428 4536 5494 442 Size: 67 1108 958 1753 Fragments arranged by size: 1753 1177 1108 958 502 416 399 265 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6805 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3433 3433 Size: 6805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 2636 3428 3602 3647 5465 1239 Size: 1385 12 792 174 45 1818 2579 Fragments arranged by size: 2579 1818 1385 792 174 45 12 Bpu10I CC'TnA_GC Cuts at: 2129 3146 2129 Size: 1017 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2593 3218 4930 5795 6093 2593 Size: 625 1712 865 298 3305 Fragments arranged by size: 3305 1712 865 625 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5468 882 Size: 617 3969 2219 Fragments arranged by size: 3969 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4596 493 Size: 711 3392 2702 Fragments arranged by size: 3392 2702 711 BsaBI GATnn'nnATC Cuts at: 3176 6799 3176 Size: 3623 3182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4612 4994 5629 275 Size: 53 83 186 936 3079 382 635 1451 Fragments arranged by size: 3079 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3750 5238 5636 5910 6057 6468 3750 Size: 1488 398 274 147 411 4087 Fragments arranged by size: 4087 1488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 1178 Size: 30 165 30 1272 30 5278 Fragments arranged by size: 5278 1272 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2438 2441 2729 2744 2891 3644 1186 Size: 943 309 3 288 15 147 753 4347 Fragments arranged by size: 4347 943 753 309 288 147 15 3 BseYI C'CCAG_C Cuts at: 2353 2461 2472 2649 3185 4073 6008 6504 2353 Size: 108 11 177 536 888 1935 496 2654 Fragments arranged by size: 2654 1935 888 536 496 177 108 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 3059 3455 3460 3476 1163 Size: 573 291 1032 396 5 16 4492 Fragments arranged by size: 4492 1032 573 396 291 16 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4255 5016 6044 1540 Size: 275 186 2254 761 1028 2301 Fragments arranged by size: 2301 2254 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2622 3234 3335 4866 4951 6022 2622 Size: 612 101 1531 85 1071 3405 Fragments arranged by size: 3405 1531 1071 612 101 85 BsmI GAATG_Cn' Cuts at: 4316 4409 4316 Size: 93 6712 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2622 2650 2772 2811 3102 3206 Size: 399 347 485 28 122 39 291 104 Cuts at: 3206 3234 3335 3416 3737 4124 4866 4951 6022 Size: 28 101 81 321 387 742 85 1071 Cuts at: 6022 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 485 399 387 347 321 291 122 104 101 85 81 39 28 28 BspEI T'CCGG_A Cuts at: 3750 6468 3750 Size: 2718 4087 BspHI T'CATG_A Cuts at: 4694 6424 4694 Size: 1730 5075 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3979 4160 844 Size: 3135 181 3489 Fragments arranged by size: 3489 3135 181 BsrBI CCG'CTC Cuts at: 1394 1823 4692 1394 Size: 429 2869 3507 Fragments arranged by size: 3507 2869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2213 5300 5474 65 Size: 1183 965 3087 174 1396 Fragments arranged by size: 3087 1396 1183 965 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2349 5449 5636 1700 Size: 110 539 3100 187 2869 Fragments arranged by size: 3100 2869 539 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6805 BssSI C'ACGA_G Cuts at: 4865 5877 4865 Size: 1012 5793 BstAPI GCAn_nnn'nTGC Cuts at: 794 2442 3070 794 Size: 1648 628 4529 Fragments arranged by size: 4529 1648 628 BstBI TT'CG_AA Cuts at: 5609 5609 Size: 6805 BstEII G'GTnAC_C Cuts at: 1184 3457 1184 Size: 2273 4532 BstYI r'GATC_y Cuts at: 1972 2029 2782 4135 4502 4887 4904 6356 6442 Size: 57 753 1353 367 385 17 1452 86 Cuts at: 6442 6454 6800 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1353 753 385 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2397 2997 2397 Size: 600 6205 BtgI C'CryG_G Cuts at: 513 1065 2258 2655 3537 513 Size: 552 1193 397 882 3781 Fragments arranged by size: 3781 1193 882 552 397 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6066 Fragments arranged by size: 6066 683 56 BtsI GCAGTG_nn' Cuts at: 2324 2369 2799 3467 3806 3942 4321 5115 5135 Size: 45 430 668 339 136 379 794 20 Cuts at: 5135 2324 Size: 3994 Fragments arranged by size: 3994 794 668 430 379 339 136 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6805 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6770 DraI TTT'AAA Cuts at: 4109 4109 Size: 6805 DraIII CAC_nnn'GTG Cuts at: 2458 2458 Size: 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2282 5812 809 Size: 507 966 3530 1802 Fragments arranged by size: 3530 1802 966 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 3127 4252 Size: 54 1425 50 275 18 345 952 1125 Cuts at: 4252 5141 6770 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1125 952 889 345 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4252 1537 Size: 275 2440 4090 Fragments arranged by size: 4090 2440 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3242 3704 3856 4735 1353 Size: 1889 462 152 879 3423 Fragments arranged by size: 3423 1889 879 462 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2029 3079 3745 5392 5766 5912 Size: 1064 138 440 1050 666 1647 374 146 Cuts at: 5912 387 Size: 1280 Fragments arranged by size: 1647 1280 1064 1050 666 440 374 146 138 EcoICRI GAG'CTC Cuts at: 2620 2620 Size: 6805 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2624 2636 2952 3213 3428 3602 Size: 393 731 261 12 316 261 215 174 Cuts at: 3602 3620 3647 4088 4868 5465 6252 6520 1239 Size: 18 27 441 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 731 597 441 393 316 268 261 261 215 174 27 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2249 2996 3629 2249 Size: 747 633 5425 Fragments arranged by size: 5425 747 633 EcoO109I rG'GnC_Cy Cuts at: 2133 2134 2133 Size: 1 6804 EcoRI G'AATT_C Cuts at: 2819 4114 4492 2819 Size: 1295 378 5132 Fragments arranged by size: 5132 1295 378 FspI TGC'GCA Cuts at: 5311 5311 Size: 6805 HaeII r_GCGC'y Cuts at: 1678 5952 1678 Size: 4274 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2990 3022 5446 5478 5520 Size: 32 135 32 1418 32 2424 32 42 Cuts at: 5520 5552 1373 Size: 32 2626 Fragments arranged by size: 2626 2424 1418 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4149 4395 669 Size: 988 281 2211 246 3079 Fragments arranged by size: 3079 2211 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6805 HpaI GTT'AAC Cuts at: 4395 4395 Size: 6805 KpnI G_GTAC'C Cuts at: 4130 4130 Size: 6805 MfeI C'AATT_G Cuts at: 4404 4404 Size: 6805 MluI A'CGCG_T Cuts at: 6681 6681 Size: 6805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2934 5919 6103 1305 Size: 1629 2985 184 2007 Fragments arranged by size: 2985 2007 1629 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 3129 10 Size: 54 1425 343 345 952 3686 Fragments arranged by size: 3686 1425 952 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 4763 5122 5281 518 Size: 648 2268 1329 359 159 2042 Fragments arranged by size: 2268 2042 1329 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2472 2912 3043 4221 4898 6046 6291 Size: 522 344 440 131 1178 677 1148 245 Cuts at: 6291 1606 Size: 2120 Fragments arranged by size: 2120 1178 1148 677 522 440 344 245 131 NaeI GCC'GGC Cuts at: 2351 2351 Size: 6805 NcoI C'CATG_G Cuts at: 513 1065 2258 3537 513 Size: 552 1193 1279 3781 Fragments arranged by size: 3781 1279 1193 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6805 NgoMIV G'CCGG_C Cuts at: 2349 2349 Size: 6805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6805 NotI GC'GGCC_GC Cuts at: 4252 4252 Size: 6805 NspI r_CATG'y Cuts at: 1132 1229 3506 4163 5708 1132 Size: 97 2277 657 1545 2229 Fragments arranged by size: 2277 2229 1545 657 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6805 PciI A'CATG_T Cuts at: 5704 5704 Size: 6805 PflMI CCAn_nnn'nTGG Cuts at: 1108 3451 3990 1108 Size: 2343 539 3923 Fragments arranged by size: 3923 2343 539 PfoI T'CCnGG_A Cuts at: 3300 3300 Size: 6805 PmeI GTTT'AAAC Cuts at: 4109 4109 Size: 6805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4874 4906 6387 6419 4874 Size: 32 1481 32 5260 Fragments arranged by size: 5260 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6805 PsiI TTA'TAA Cuts at: 4375 4375 Size: 6805 PspOMI G'GGCC_C Cuts at: 2133 2133 Size: 6805 PspXI vC'TCGA_Gb Cuts at: 2621 2621 Size: 6805 PstI C_TGCA'G Cuts at: 830 3013 4157 830 Size: 2183 1144 3478 Fragments arranged by size: 3478 2183 1144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6805 PvuII CAG'CTG Cuts at: 2128 3043 2128 Size: 915 5890 SacI G_AGCT'C Cuts at: 2622 2622 Size: 6805 SalI G'TCGA_C Cuts at: 1936 4147 1936 Size: 2211 4594 SbfI CC_TGCA'GG Cuts at: 4157 4157 Size: 6805 ScaI AGT'ACT Cuts at: 3533 5053 6765 3533 Size: 1520 1712 3573 Fragments arranged by size: 3573 1712 1520 SfcI C'TryA_G Cuts at: 826 1045 3009 4153 5288 5969 6160 826 Size: 219 1964 1144 1135 681 191 1471 Fragments arranged by size: 1964 1471 1144 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6805 SmaI CCC'GGG Cuts at: 1872 4132 1872 Size: 2260 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2572 2621 3233 4909 5810 6072 Size: 197 926 629 49 612 1676 901 262 Cuts at: 6072 820 Size: 1553 Fragments arranged by size: 1676 1553 926 901 629 612 262 197 49 SnaBI TAC'GTA Cuts at: 493 4596 493 Size: 4103 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6805 SphI G_CATG'C Cuts at: 1132 4163 1132 Size: 3031 3774 SspI AAT'ATT Cuts at: 5 52 4729 5 Size: 47 4677 2081 Fragments arranged by size: 4677 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6805 StyI C'CwwG_G Cuts at: 513 1065 2258 2811 3537 4244 513 Size: 552 1193 553 726 707 3074 Fragments arranged by size: 3074 1193 726 707 553 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 2869 3125 4832 4849 5002 5187 Size: 1255 855 434 256 1707 17 153 185 Cuts at: 5187 5632 325 Size: 445 1498 Fragments arranged by size: 1707 1498 1255 855 445 434 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2604 3531 Size: 274 80 33 51 1443 237 390 927 Cuts at: 3531 5051 6763 96 Size: 1520 1712 138 Fragments arranged by size: 1712 1520 1443 927 390 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 3238 3270 6765 6797 2075 Size: 32 1131 32 3495 32 2083 Fragments arranged by size: 3495 2083 1131 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2246 1316 Size: 930 5875 XbaI T'CTAG_A Cuts at: 4141 4141 Size: 6805 XhoI C'TCGA_G Cuts at: 1943 2621 1943 Size: 678 6127 XmaI C'CCGG_G Cuts at: 1870 4130 1870 Size: 2260 4545 XmnI GAAnn'nnTTC Cuts at: 4934 4934 Size: 6805 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4613 276 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI