################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbrPI BbsI BcgI BclI BfrI BglII BpuAI BsaAI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I MaeII MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 824 GGTACC G^GTACC AccI 1345 GTATAC GT^[AC][GT]AC AccIII 951 TCCGGA T^CCGGA AgeI 868 ACCGGT A^CCGGT AlwI 1239 GATCC GGATC(4/5) AlwNI 1057 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 824 GGTACC G^GTACC AvrII 130 CCTAGG C^CTAGG BalI 1398 TGGCCA TGG^CCA BbvCI 351 CCTCAGC CCTCAGC(-5/-2) BciVI 690 GTATCC GTATCC(6/5) BlnI 130 CCTAGG C^CTAGG BseAI 951 TCCGGA T^CCGGA BsgI 1388 GTGCAG GTGCAG(16/14) BsiEI 45 CGGCCG CG[GA][CT]^CG BsmAI 759 GAGAC GTCTC(1/5) BsmBI 759 GAGACG CGTCTC(1/5) BspEI 951 TCCGGA T^CCGGA Bst1107I 1345 GTATAC GTA^TAC BstYI 1238 AGATCC [GA]^GATC[CT] BstZ17I 1345 GTATAC GTA^TAC DpnII 1239 GATC ^GATC EagI 45 CGGCCG C^GGCCG EclXI 45 CGGCCG C^GGCCG Eco52I 45 CGGCCG C^GGCCG EcoRV 1446 GATATC GAT^ATC EcoT22I 1284 ATGCAT ATGCA^T HinfI 61 GAATC G^A[ACGT]TC KpnI 824 GGTACC GGTAC^C MboI 1239 GATC ^GATC MflI 1238 AGATCC [GA]^GATC[CT] MluNI 1398 TGGCCA TGG^CCA MroI 951 TCCGGA T^CCGGA MscI 1398 TGGCCA TGG^CCA MslI 492 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 1239 GATC ^GATC NsiI 1284 ATGCAT ATGCA^T NspI 1571 ACATGC [GA]CATG^[CT] PflMI 1056 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 868 ACCGGT A^CCGGT PstI 471 CTGCAG CTGCA^G Sau3AI 1239 GATC ^GATC SexAI 936 ACCAGGT A^CC[AT]GGT SfiI 957 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 867 CACCGGTG C[GA]^CCGG[CT]G TfiI 61 GAATC G^A[AT]TC Tsp509I 1372 AATT ^AATT Van91I 1056 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 23 CCAGTCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1238 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BlpI 214 GCTGAGC GC^T[ACGT]AGC 646 GCTCAGC Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC 646 GCTCAGC BsaWI 868 ACCGGT [AT]^CCGG[AT] 951 TCCGGA BseRI 149 GAGGAG GAGGAG(10/8) 928 GAGGAG BsrDI 1046 GCAATG GCAATG(2/0) 1249 GCAATG CelII 214 GCTGAGC GC^T[ACGT]AGC 646 GCTCAGC DraIII 784 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1080 CACCCAGTG HgaI 211 GACGC GACGC(5/10) 391 GACGC MboII 1165 GAAGA GAAGA(8/7) 1236 GAAGA NaeI 186 GCCGGC GCC^GGC 464 GCCGGC NcoI 1451 CCATGG C^CATGG 1560 CCATGG NgoMIV 186 GCCGGC G^CCGGC 464 GCCGGC PvuII 1196 CAGCTG CAG^CTG 1504 CAGCTG SacI 616 GAGCTC GAGCT^C 716 GAGCTC SfcI 471 CTGCAG C^T[GA][CT]AG 1134 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PpuMI RsaI SacII Sau96I ScrFI SfaNI SfoI SmaI StyI TseI Tsp45I TspRI XmaCI XmaI