(Circular) (Six-base) MAPSORT of: pFN21AE1369.seq Check: 5998 from: 1 to: 6319 pFN21AE1369 6319 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3351 3662 1937 Size: 1414 311 4594 Fragments arranged by size: 4594 1414 311 Acc65I G'GTAC_C Cuts at: 2829 3640 2829 Size: 811 5508 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4382 5766 6034 1632 Size: 2750 1384 268 1917 Fragments arranged by size: 2750 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 2873 5150 2873 Size: 2277 4042 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2447 3069 1184 Size: 1263 622 4434 Fragments arranged by size: 4434 1263 622 AlwNI CAG_nnn'CTG Cuts at: 3067 4062 5634 3067 Size: 995 1572 3752 Fragments arranged by size: 3752 1572 995 ApaI G_GGCC'C Cuts at: 2151 2757 2974 2151 Size: 606 217 5496 Fragments arranged by size: 5496 606 217 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2151 2196 2292 2418 2908 2967 Size: 73 153 55 45 96 126 490 59 Cuts at: 2967 3644 1870 Size: 677 4545 Fragments arranged by size: 4545 677 490 153 126 96 73 59 55 45 AvrII C'CTAG_G Cuts at: 2135 2135 Size: 6319 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2248 2822 2829 2870 Size: 325 217 627 325 136 574 7 41 Cuts at: 2870 3011 3640 5095 618 Size: 141 629 1455 1842 Fragments arranged by size: 1842 1455 629 627 574 325 325 217 141 136 41 7 BanII G_rGCy'C Cuts at: 1391 2151 2343 2531 2625 2725 2757 2896 2974 Size: 760 192 188 94 100 32 139 78 Cuts at: 2974 3638 1391 Size: 664 4072 Fragments arranged by size: 4072 760 664 192 188 139 100 94 78 32 BbeI G_GCGC'C Cuts at: 2116 2252 2826 2116 Size: 136 574 5609 Fragments arranged by size: 5609 574 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5348 BbvCI CC'TCA_GC Cuts at: 2357 2357 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2706 4211 5421 2706 Size: 1505 1210 3604 Fragments arranged by size: 3604 1505 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BfrBI ATG'CAT Cuts at: 3291 3291 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2421 2542 2556 Size: 107 122 71 631 605 749 121 14 Cuts at: 2556 2969 3062 3315 4930 136 Size: 413 93 253 1615 1525 Fragments arranged by size: 1615 1525 749 631 605 413 253 122 121 107 93 71 14 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 2220 2652 3736 2220 Size: 432 1084 4803 Fragments arranged by size: 4803 1084 432 Bme1580I G_kGCm'C Cuts at: 1790 2151 2757 2873 2974 3555 4380 5536 1790 Size: 361 606 116 101 581 825 1156 2573 Fragments arranged by size: 2573 1156 825 606 581 361 116 101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2172 3082 4050 Size: 416 53 83 399 24 755 910 968 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 968 958 910 755 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2357 2481 2739 2951 4979 1239 Size: 821 297 124 258 212 2028 2579 Fragments arranged by size: 2579 2028 821 297 258 212 124 Bpu10I CC'TnA_GC Cuts at: 2357 2357 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2113 2249 2823 4126 Size: 53 83 186 936 580 136 574 1303 Cuts at: 4126 4508 5143 275 Size: 382 635 1451 Fragments arranged by size: 1451 1303 936 635 580 574 382 186 136 83 53 BsaWI w'CCGG_w Cuts at: 2873 2956 4752 5150 5424 5571 5982 2873 Size: 83 1796 398 274 147 411 3210 Fragments arranged by size: 3210 1796 411 398 274 147 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3399 3429 1178 Size: 30 165 30 1996 30 4068 Fragments arranged by size: 4068 1996 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 2948 1186 Size: 983 779 4557 Fragments arranged by size: 4557 983 779 BseYI C'CCAG_C Cuts at: 5522 6018 5522 Size: 496 5823 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3414 1163 Size: 573 1678 4068 Fragments arranged by size: 4068 1678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2053 3769 4530 5558 1540 Size: 275 186 52 1716 761 1028 2301 Fragments arranged by size: 2301 1716 1028 761 275 186 52 BsiHKAI G_wGCw'C Cuts at: 2578 2625 2725 4380 4465 5536 2578 Size: 47 100 1655 85 1071 3361 Fragments arranged by size: 3361 1655 1071 100 85 47 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 2758 2758 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2343 2531 2578 2625 2725 2757 Size: 399 361 192 188 47 47 100 32 Cuts at: 2757 2873 2896 2974 3555 3638 4380 4465 5536 Size: 116 23 78 581 83 742 85 1071 Cuts at: 5536 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 581 399 361 192 188 116 100 85 83 78 47 47 32 23 BspEI T'CCGG_A Cuts at: 2956 5982 2956 Size: 3026 3293 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3674 844 Size: 2830 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2367 2747 4206 1394 Size: 429 188 356 380 1459 3507 Fragments arranged by size: 3507 1459 429 380 356 188 BsrDI GCAATG_nn' Cuts at: 65 1248 3058 3261 4814 4988 65 Size: 1183 1810 203 1553 174 1396 Fragments arranged by size: 1810 1553 1396 1183 203 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2073 2191 2469 2578 2798 2873 4963 Size: 110 263 118 278 109 220 75 2090 Cuts at: 4963 5150 1700 Size: 187 2869 Fragments arranged by size: 2869 2090 278 263 220 187 118 110 109 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 3057 3297 3306 794 Size: 2263 240 9 3807 Fragments arranged by size: 3807 2263 240 9 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 3243 3649 4016 4401 4418 5870 5956 5968 Size: 1271 406 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1271 406 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3352 3352 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 2274 2343 2521 2661 2817 2896 Size: 552 939 270 69 178 140 156 79 Cuts at: 2896 3456 3565 513 Size: 560 109 3267 Fragments arranged by size: 3267 939 560 552 270 178 156 140 109 79 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2794 3090 2794 Size: 296 6023 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2388 2568 Size: 54 1425 50 275 18 220 338 180 Cuts at: 2568 3403 3766 4655 6284 8 Size: 835 363 889 1629 43 Fragments arranged by size: 1629 1425 889 835 363 338 275 220 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2050 3766 1537 Size: 275 238 1716 4090 Fragments arranged by size: 4090 1716 275 238 EarI CTCTTCn'nnn_ Cuts at: 1353 4249 1353 Size: 2896 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2469 2599 4906 5280 5426 387 Size: 1064 138 880 130 2307 374 146 1280 Fragments arranged by size: 2307 1280 1064 880 374 146 138 130 EcoICRI GAG'CTC Cuts at: 2623 2723 2623 Size: 100 6219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2357 2481 2739 2951 4382 4979 Size: 393 428 297 124 258 212 1431 597 Cuts at: 4979 5766 6034 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1431 787 597 428 393 297 268 258 212 124 EcoO109I rG'GnC_Cy Cuts at: 2091 2132 2147 2148 2168 2602 2753 2754 2945 Size: 41 15 1 20 434 151 1 191 Cuts at: 2945 3064 2091 Size: 119 5346 Fragments arranged by size: 5346 434 191 151 119 41 20 15 1 1 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 3453 3453 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2116 2252 2521 2826 5466 1678 Size: 438 136 269 305 2640 2531 Fragments arranged by size: 2640 2531 438 305 269 136 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373 Size: 32 135 32 3388 32 42 32 2626 Fragments arranged by size: 3388 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 2112 2248 2822 2112 Size: 136 574 5609 Fragments arranged by size: 5609 574 136 KpnI G_GTAC'C Cuts at: 2833 3644 2833 Size: 811 5508 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 3366 5433 5617 1305 Size: 861 1200 2067 184 2007 Fragments arranged by size: 2067 2007 1200 861 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3405 10 Size: 54 1425 343 1573 2924 Fragments arranged by size: 2924 1573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 4277 4636 4795 518 Size: 648 1335 1776 359 159 2042 Fragments arranged by size: 2042 1776 1335 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2493 2540 2656 2663 2898 3203 3511 Size: 670 217 47 116 7 235 305 308 Cuts at: 3511 3583 3735 4412 5560 5805 1606 Size: 72 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 670 308 305 245 235 217 152 116 72 47 7 NaeI GCC'GGC Cuts at: 2193 2471 2193 Size: 278 6041 NarI GG'CG_CC Cuts at: 2113 2249 2823 2113 Size: 136 574 5609 Fragments arranged by size: 5609 574 136 NcoI C'CATG_G Cuts at: 513 1065 2004 3456 3565 513 Size: 552 939 1452 109 3267 Fragments arranged by size: 3267 1452 939 552 109 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2191 2469 2191 Size: 278 6041 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NsiI A_TGCA'T Cuts at: 3293 3293 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3580 3677 5222 1132 Size: 97 2351 97 1545 2229 Fragments arranged by size: 2351 2229 1545 97 97 PasI CC'CwG_GG Cuts at: 1348 2707 1348 Size: 1359 4960 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 3067 1108 Size: 1959 4360 PfoI T'CCnGG_A Cuts at: 2593 2593 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2132 2168 2602 2945 2091 Size: 41 36 434 343 5465 Fragments arranged by size: 5465 434 343 41 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2147 2753 2970 2147 Size: 606 217 5496 Fragments arranged by size: 5496 606 217 PstI C_TGCA'G Cuts at: 830 2480 3671 830 Size: 1650 1191 3478 Fragments arranged by size: 3478 1650 1191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 3203 3511 3203 Size: 308 6011 SacI G_AGCT'C Cuts at: 2625 2725 2625 Size: 100 6219 SacII CC_GC'GG Cuts at: 2277 2664 2899 2277 Size: 387 235 5697 Fragments arranged by size: 5697 387 235 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 2091 2602 2091 Size: 511 5808 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2941 2941 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2476 3139 3667 4802 5483 5674 826 Size: 219 1431 663 528 1135 681 191 1471 Fragments arranged by size: 1471 1431 1135 681 663 528 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2969 2969 Size: 6319 SfoI GGC'GCC Cuts at: 2114 2250 2824 2114 Size: 136 574 5609 Fragments arranged by size: 5609 574 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 2873 1700 Size: 1173 5146 SmaI CCC'GGG Cuts at: 1872 2098 2198 2294 2420 2969 3646 1872 Size: 226 100 96 126 549 677 4545 Fragments arranged by size: 4545 677 549 226 126 100 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 4423 5324 5586 820 Size: 197 926 2480 901 262 1553 Fragments arranged by size: 2480 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SrfI GCCC'GGGC Cuts at: 2969 2969 Size: 6319 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 2135 2757 3456 3565 3758 513 Size: 552 939 131 622 699 109 193 3074 Fragments arranged by size: 3074 939 699 622 552 193 131 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2655 3101 4346 4363 4516 4701 5146 Size: 1255 1075 446 1245 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 1498 1255 1245 1075 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3159 4565 6277 Size: 274 80 33 51 1443 1182 1406 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 1712 1443 1406 1182 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2035 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2096 2196 2292 2418 2967 3644 1870 Size: 226 100 96 126 549 677 4545 Fragments arranged by size: 4545 677 549 226 126 100 96 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BplI BsiWI BstXI Bsu36I EcoNI FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SapI SwaI