(Linear) (Six-base) MAPSORT of: KIEE1369.seq Check: 8639 from: 1 to: 1614 KIEE1369.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AccI GT'mk_AC Cuts at: 0 1346 1614 Size: 1346 268 Acc65I G'GTAC_C Cuts at: 0 824 1614 Size: 824 790 AgeI A'CCGG_T Cuts at: 0 868 1614 Size: 868 746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 442 1064 1614 Size: 442 622 550 Fragments arranged by size: 622 550 442 AlwNI CAG_nnn'CTG Cuts at: 0 1062 1614 Size: 1062 552 ApaI G_GGCC'C Cuts at: 0 146 752 969 1614 Size: 146 606 217 645 Fragments arranged by size: 645 606 217 146 AvaI C'yCGr_G Cuts at: 0 91 146 191 287 413 903 962 1614 Size: 91 55 45 96 126 490 59 652 Fragments arranged by size: 652 490 126 96 91 59 55 45 AvrII C'CTAG_G Cuts at: 0 130 1614 Size: 130 1484 BanI G'GyrC_C Cuts at: 0 107 243 817 824 865 1006 1614 Size: 107 136 574 7 41 141 608 Fragments arranged by size: 608 574 141 136 107 41 7 BanII G_rGCy'C Cuts at: 0 146 338 526 620 720 752 891 969 Size: 146 192 188 94 100 32 139 78 Cuts at: 969 1614 Size: 645 Fragments arranged by size: 645 192 188 146 139 100 94 78 32 BbeI G_GCGC'C Cuts at: 0 111 247 821 1614 Size: 111 136 574 793 Fragments arranged by size: 793 574 136 111 BbvCI CC'TCA_GC Cuts at: 0 352 1614 Size: 352 1262 BciVI GTATCCnnnnn_n' Cuts at: 0 701 1614 Size: 701 913 BfrBI ATG'CAT Cuts at: 0 1286 1614 Size: 1286 328 BglI GCCn_nnn'nGGC Cuts at: 0 416 537 551 964 1057 1310 1614 Size: 416 121 14 413 93 253 304 Fragments arranged by size: 416 413 304 253 121 93 14 BlpI GC'TnA_GC Cuts at: 0 215 647 1614 Size: 215 432 967 Fragments arranged by size: 967 432 215 Bme1580I G_kGCm'C Cuts at: 0 146 752 868 969 1550 1614 Size: 146 606 116 101 581 64 Fragments arranged by size: 606 581 146 116 101 64 BmrI ACTGGGnnnn_n' Cuts at: 0 167 1077 1614 Size: 167 910 537 Fragments arranged by size: 910 537 167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 352 476 734 946 1614 Size: 55 297 124 258 212 668 Fragments arranged by size: 668 297 258 212 124 55 Bpu10I CC'TnA_GC Cuts at: 0 352 1614 Size: 352 1262 BsaHI Gr'CG_yC Cuts at: 0 108 244 818 1614 Size: 108 136 574 796 Fragments arranged by size: 796 574 136 108 BsaWI w'CCGG_w Cuts at: 0 868 951 1614 Size: 868 83 663 Fragments arranged by size: 868 663 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1394 1424 1614 Size: 1394 30 190 Fragments arranged by size: 1394 190 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 164 943 1614 Size: 164 779 671 Fragments arranged by size: 779 671 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1409 1614 Size: 1409 205 BsiEI CG_ry'CG Cuts at: 0 48 1614 Size: 48 1566 BsiHKAI G_wGCw'C Cuts at: 0 573 620 720 1614 Size: 573 47 100 894 Fragments arranged by size: 894 573 100 47 BsmBI CGTCTCn'nnnn_ Cuts at: 0 753 1614 Size: 753 861 Bsp1286I G_dGCh'C Cuts at: 0 146 338 526 573 620 720 752 868 Size: 146 192 188 47 47 100 32 116 Cuts at: 868 891 969 1550 1614 Size: 23 78 581 64 Fragments arranged by size: 581 192 188 146 116 100 78 64 47 47 32 23 BspEI T'CCGG_A Cuts at: 0 951 1614 Size: 951 663 BsrBI CCG'CTC Cuts at: 0 6 362 742 1614 Size: 6 356 380 872 Fragments arranged by size: 872 380 356 6 BsrDI GCAATG_nn' Cuts at: 0 1053 1256 1614 Size: 1053 203 358 Fragments arranged by size: 1053 358 203 BsrFI r'CCGG_y Cuts at: 0 68 186 464 573 793 868 1614 Size: 68 118 278 109 220 75 746 Fragments arranged by size: 746 278 220 118 109 75 68 BstAPI GCAn_nnn'nTGC Cuts at: 0 1052 1292 1301 1614 Size: 1052 240 9 313 Fragments arranged by size: 1052 313 240 9 BstYI r'GATC_y Cuts at: 0 1238 1614 Size: 1238 376 BstZ17I GTA'TAC Cuts at: 0 1347 1614 Size: 1347 267 BtgI C'CryG_G Cuts at: 0 269 338 516 656 812 891 1451 1560 Size: 269 69 178 140 156 79 560 109 Cuts at: 1560 1614 Size: 54 Fragments arranged by size: 560 269 178 156 140 109 79 69 54 DraIII CAC_nnn'GTG Cuts at: 0 789 1085 1614 Size: 789 296 529 Fragments arranged by size: 789 529 296 EaeI y'GGCC_r Cuts at: 0 45 383 563 1398 1614 Size: 45 338 180 835 216 Fragments arranged by size: 835 338 216 180 45 EagI C'GGCC_G Cuts at: 0 45 1614 Size: 45 1569 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 464 594 1614 Size: 464 130 1020 Fragments arranged by size: 1020 464 130 EcoICRI GAG'CTC Cuts at: 0 618 718 1614 Size: 618 100 896 Fragments arranged by size: 896 618 100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 352 476 734 946 1614 Size: 55 297 124 258 212 668 Fragments arranged by size: 668 297 258 212 124 55 EcoO109I rG'GnC_Cy Cuts at: 0 86 127 142 143 163 597 748 749 Size: 86 41 15 1 20 434 151 1 Cuts at: 749 940 1059 1614 Size: 191 119 555 Fragments arranged by size: 555 434 191 151 119 86 41 20 15 1 1 EcoRV GAT'ATC Cuts at: 0 1448 1614 Size: 1448 166 HaeII r_GCGC'y Cuts at: 0 111 247 516 821 1614 Size: 111 136 269 305 793 Fragments arranged by size: 793 305 269 136 111 KasI G'GCGC_C Cuts at: 0 107 243 817 1614 Size: 107 136 574 797 Fragments arranged by size: 797 574 136 107 KpnI G_GTAC'C Cuts at: 0 828 1614 Size: 828 786 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 161 1361 1614 Size: 161 1200 253 Fragments arranged by size: 1200 253 161 MscI TGG'CCA Cuts at: 0 1400 1614 Size: 1400 214 MslI CAynn'nnrTG Cuts at: 0 496 1614 Size: 496 1118 MspA1I CmG'CkG Cuts at: 0 271 488 535 651 658 893 1198 1506 Size: 271 217 47 116 7 235 305 308 Cuts at: 1506 1578 1614 Size: 72 36 Fragments arranged by size: 308 305 271 235 217 116 72 47 36 7 NaeI GCC'GGC Cuts at: 0 188 466 1614 Size: 188 278 1148 Fragments arranged by size: 1148 278 188 NarI GG'CG_CC Cuts at: 0 108 244 818 1614 Size: 108 136 574 796 Fragments arranged by size: 796 574 136 108 NcoI C'CATG_G Cuts at: 0 1451 1560 1614 Size: 1451 109 54 Fragments arranged by size: 1451 109 54 NgoMIV G'CCGG_C Cuts at: 0 186 464 1614 Size: 186 278 1150 Fragments arranged by size: 1150 278 186 NsiI A_TGCA'T Cuts at: 0 1288 1614 Size: 1288 326 NspI r_CATG'y Cuts at: 0 1575 1614 Size: 1575 39 PasI CC'CwG_GG Cuts at: 0 702 1614 Size: 702 912 PflMI CCAn_nnn'nTGG Cuts at: 0 1062 1614 Size: 1062 552 PfoI T'CCnGG_A Cuts at: 0 588 1614 Size: 588 1026 PpuMI rG'GwC_Cy Cuts at: 0 86 127 163 597 940 1614 Size: 86 41 36 434 343 674 Fragments arranged by size: 674 434 343 86 41 36 PspOMI G'GGCC_C Cuts at: 0 142 748 965 1614 Size: 142 606 217 649 Fragments arranged by size: 649 606 217 142 PstI C_TGCA'G Cuts at: 0 475 1614 Size: 475 1139 PvuII CAG'CTG Cuts at: 0 1198 1506 1614 Size: 1198 308 108 Fragments arranged by size: 1198 308 108 SacI G_AGCT'C Cuts at: 0 620 720 1614 Size: 620 100 894 Fragments arranged by size: 894 620 100 SacII CC_GC'GG Cuts at: 0 272 659 894 1614 Size: 272 387 235 720 Fragments arranged by size: 720 387 272 235 SanDI GG'GwC_CC Cuts at: 0 86 597 1614 Size: 86 511 1017 Fragments arranged by size: 1017 511 86 SexAI A'CCwGG_T Cuts at: 0 936 1614 Size: 936 678 SfcI C'TryA_G Cuts at: 0 471 1134 1614 Size: 471 663 480 Fragments arranged by size: 663 480 471 SfiI GGCCn_nnn'nGGCC Cuts at: 0 964 1614 Size: 964 650 SfoI GGC'GCC Cuts at: 0 109 245 819 1614 Size: 109 136 574 795 Fragments arranged by size: 795 574 136 109 SgrAI Cr'CCGG_yG Cuts at: 0 868 1614 Size: 868 746 SmaI CCC'GGG Cuts at: 0 93 193 289 415 964 1614 Size: 93 100 96 126 549 650 Fragments arranged by size: 650 549 126 100 96 93 SrfI GCCC'GGGC Cuts at: 0 964 1614 Size: 964 650 StyI C'CwwG_G Cuts at: 0 130 752 1451 1560 1614 Size: 130 622 699 109 54 Fragments arranged by size: 699 622 130 109 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 650 1096 1614 Size: 650 446 518 Fragments arranged by size: 650 518 446 TatI w'GTAC_w Cuts at: 0 1154 1614 Size: 1154 460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 30 1614 Size: 30 1584 XmaI C'CCGG_G Cuts at: 0 91 191 287 413 962 1614 Size: 91 100 96 126 549 652 Fragments arranged by size: 652 549 126 100 96 91 Enzymes that do cut: AccI Acc65I AgeI AlfI AlwNI ApaI AvaI AvrII BanI BanII BbeI BbvCI BciVI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BstAPI BstYI BstZ17I BtgI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfiI SfoI SgrAI SmaI SrfI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AflIII AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI BcgI BclI BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI BsiWI BsmI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoNI EcoRI FalI FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI