################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MflI MluI MseI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 175 CGCG CG^CG AccIII 738 TCCGGA T^CCGGA AflIII 226 ACATGT A^C[GA][CT]GT AocI 750 CCTGAGG CC^T[ACGT]AGG AspHI 923 GAGCTC G[AT]GC[AT]^C BbeI 860 GGCGCC GGCGC^C BbvCI 641 CCTCAGC CCTCAGC(-5/-2) BclI 440 TGATCA T^GATCA BsaI 366 GGTCTC GGTCTC(1/5) BseAI 738 TCCGGA T^CCGGA BsiHKAI 923 GAGCTC G[AT]GC[AT]^C BspEI 738 TCCGGA T^CCGGA BspLU11I 226 ACATGT A^CATGT BspMI 134 ACCTGC ACCTGC(4/8) BsrFI 1184 GCCGGC [GA]^CCGG[CT] BstEII 934 GGTGACC G^GT[ACGT]ACC BstPI 934 GGTGACC G^GT[ACGT]ACC BstUI 175 CGCG CG^CG BstXI 419 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 750 CCTGAGG CC^T[ACGT]AGG Cfr10I 1184 GCCGGC [GA]^CCGG[CT] DraII 961 AGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 750 CCTGAGG CC^T[ACGT]AGG EcoNI 1197 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 961 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 934 GGTGACC G^GT[ACGT]ACC FbaI 440 TGATCA T^GATCA HaeII 860 GGCGCC [GA]GCGC^[CT] HgaI 353 GCGTC GACGC(5/10) HincII 454 GTCAAC GT[CT]^[GA]AC HindII 454 GTCAAC GT[CT]^[GA]AC KasI 860 GGCGCC G^GCGCC KspI 174 CCGCGG CCGC^GG MfeI 1248 CAATTG C^AATTG MroI 738 TCCGGA T^CCGGA MunI 1248 CAATTG C^AATTG MvnI 175 CGCG CG^CG NaeI 1184 GCCGGC GCC^GGC NarI 860 GGCGCC GG^CGCC NcoI 590 CCATGG C^CATGG NgoMIV 1184 GCCGGC G^CCGGC NheI 699 GCTAGC G^CTAGC NspI 226 ACATGT [GA]CATG^[CT] PpuMI 961 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1041 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 923 GAGCTC GAGCT^C SacII 174 CCGCGG CCGC^GG SexAI 14 ACCTGGT A^CC[AT]GGT SfoI 860 GGCGCC GGC^GCC SpeI 154 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 352 GGCGTC G[GA]^CG[CT]C 860 GGCGCC BanII 846 GGGCTC G[GA]GC[CT]^C 923 GAGCTC BbsI 158 GTCTTC GAAGAC(2/6) 806 GTCTTC BpuAI 158 GTCTTC GAAGAC(2/6) 806 GTCTTC BsaHI 352 GGCGTC G[GA]^CG[CT]C 860 GGCGCC BsaWI 218 TCCGGT [AT]^CCGG[AT] 738 TCCGGA BsmAI 969 GAGAC GTCTC(1/5) 367 GTCTC BsrBI 1255 CCGCTC CCGCTC(-3/-3) 1126 GAGCGG BsrDI 191 CATTGC GCAATG(2/0) 414 CATTGC EarI 980 CTCTTC CTCTTC(1/4) 1012 GAAGAG Hin1I 352 GGCGTC G[GA]^CG[CT]C 860 GGCGCC HinfI 179 GAGTC G^A[ACGT]TC 866 GAGTC Ksp632I 980 CTCTTC CTCTTC(1/4) 1012 GAAGAG MaeII 47 ACGT A^CGT 545 ACGT MslI 414 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1032 CATCAGAGTG PleI 179 GAGTC GAGTC(4/5) 866 GAGTC PstI 883 CTGCAG CTGCA^G 1276 CTGCAG PvuII 644 CAGCTG CAG^CTG 886 CAGCTG SfcI 883 CTGCAG C^T[GA][CT]AG 1276 CTGCAG TaqI 206 TCGA T^CGA 810 TCGA Tsp509I 1106 AATT ^AATT 1249 AATT TthHB8I 206 TCGA T^CGA 810 TCGA XcmI 419 CCATGAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 575 CCAGCAGTGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccI AciI AfaI AluI AvaII BalI BanI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TseI Tsp45I TspRI