(Circular) (Six-base) MAPSORT of: pFN21AE3942.seq Check: 9404 from: 1 to: 6004 pFN21AE3942 6004 bp With 182 enzymes: SgfI * March 28, 2012 11:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2133 3243 3297 3347 1937 Size: 196 1110 54 50 4594 Fragments arranged by size: 4594 1110 196 54 50 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3012 3137 4067 5451 5719 1632 Size: 1380 125 930 1384 268 1917 Fragments arranged by size: 1917 1384 1380 930 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2231 4903 5880 1203 Size: 1028 2672 977 1327 Fragments arranged by size: 2672 1327 1028 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 2477 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 3747 5319 3747 Size: 1572 4432 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 3313 3539 3691 5800 1493 Size: 1820 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3329 1870 Size: 73 1386 4545 Fragments arranged by size: 4545 1386 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2307 2865 2920 3325 4780 Size: 325 217 627 520 558 55 405 1455 Cuts at: 4780 618 Size: 1842 Fragments arranged by size: 1842 1455 627 558 520 405 325 217 55 BanII G_rGCy'C Cuts at: 1391 2855 2932 3323 1391 Size: 1464 77 391 4072 Fragments arranged by size: 4072 1464 391 77 BbeI G_GCGC'C Cuts at: 2869 2869 Size: 6004 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 2804 928 Size: 971 257 648 4128 Fragments arranged by size: 4128 971 648 257 BbvCI CC'TCA_GC Cuts at: 2647 2647 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 2445 1548 Size: 897 5107 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2475 3190 Size: 107 122 71 631 605 337 466 715 Cuts at: 3190 3273 4615 136 Size: 83 1342 1525 Fragments arranged by size: 1525 1342 715 631 605 466 337 122 107 83 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 2310 2329 2923 4065 5221 1790 Size: 520 19 594 1142 1156 2573 Fragments arranged by size: 2573 1156 1142 594 520 19 BmgBI CAC'GTC Cuts at: 1318 2053 1318 Size: 735 5269 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2858 3735 4693 Size: 416 53 83 399 24 1441 877 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1441 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2708 1056 Size: 1652 4352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3086 3086 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2359 2654 2896 2946 3072 4664 1239 Size: 1120 295 242 50 126 1592 2579 Fragments arranged by size: 2579 1592 1120 295 242 126 50 Bpu10I CC'TnA_GC Cuts at: 2647 2647 Size: 6004 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2377 4667 882 Size: 617 878 2290 2219 Fragments arranged by size: 2290 2219 878 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 2866 3811 4193 Size: 53 83 186 936 825 508 945 382 Cuts at: 4193 4828 275 Size: 635 1451 Fragments arranged by size: 1451 945 936 825 635 508 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2223 2743 4437 4835 5109 5256 5667 2223 Size: 520 1694 398 274 147 411 2560 Fragments arranged by size: 2560 1694 520 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2869 2899 1178 Size: 30 165 30 597 30 839 30 4283 Fragments arranged by size: 4283 839 597 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3035 3038 3092 3095 3143 1186 Size: 1849 3 54 3 48 4047 Fragments arranged by size: 4047 1849 54 48 3 3 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2333 3199 1163 Size: 573 417 180 866 3968 Fragments arranged by size: 3968 866 573 417 180 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2932 4065 4150 5221 2932 Size: 1133 85 1071 3715 Fragments arranged by size: 3715 1133 1071 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2329 2855 2923 2932 3323 4065 Size: 399 520 19 526 68 9 391 742 Cuts at: 4065 4150 5221 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 520 399 391 85 68 19 9 BspEI T'CCGG_A Cuts at: 2743 5667 2743 Size: 2924 3080 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 3359 844 Size: 1304 1211 3489 Fragments arranged by size: 3489 1304 1211 BsrBI CCG'CTC Cuts at: 1394 1823 3133 3262 3891 1394 Size: 429 1310 129 629 3507 Fragments arranged by size: 3507 1310 629 429 129 BsrDI GCAATG_nn' Cuts at: 65 1248 2195 2418 4499 4673 65 Size: 1183 947 223 2081 174 1396 Fragments arranged by size: 2081 1396 1183 947 223 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3189 4648 4835 1700 Size: 110 1379 1459 187 2869 Fragments arranged by size: 2869 1459 1379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 2939 1184 Size: 1755 4249 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2431 Size: 6004 BstYI r'GATC_y Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999 Size: 1362 367 385 17 1452 86 12 346 Cuts at: 5999 1972 Size: 1977 Fragments arranged by size: 1977 1452 1362 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2756 2756 Size: 6004 BtgI C'CryG_G Cuts at: 513 1065 2004 2179 2217 2595 513 Size: 552 939 175 38 378 3922 Fragments arranged by size: 3922 939 552 378 175 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2019 2327 2526 2590 2727 3520 4314 4334 2019 Size: 308 199 64 137 793 794 20 3689 Fragments arranged by size: 3689 794 793 308 199 137 64 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3236 3271 529 Size: 35 2672 35 3262 Fragments arranged by size: 3262 2672 35 35 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 2592 2598 Size: 54 1425 50 275 18 550 212 6 Cuts at: 2598 2664 2795 2903 3451 4340 5969 8 Size: 66 131 108 548 889 1629 43 Fragments arranged by size: 1629 1425 889 550 548 275 212 131 108 66 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2991 3012 3934 1353 Size: 1638 21 922 3423 Fragments arranged by size: 3423 1638 922 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2930 2930 Size: 6004 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2359 2654 2896 2946 3012 3072 3137 Size: 393 727 295 242 50 66 60 65 Cuts at: 3137 4067 4664 5451 5719 1239 Size: 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 727 597 393 295 268 242 66 65 60 50 EcoNI CCTnn'n_nnAGG Cuts at: 3206 3206 Size: 6004 EcoO109I rG'GnC_Cy Cuts at: 2967 2967 Size: 6004 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2869 5151 1678 Size: 1191 2282 2531 Fragments arranged by size: 2531 2282 1191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2808 2840 2869 Size: 32 135 32 943 32 261 32 29 Cuts at: 2869 2901 4645 4677 4719 4751 1373 Size: 32 1744 32 42 32 2626 Fragments arranged by size: 2626 1744 943 261 135 42 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2461 3348 3594 669 Size: 988 281 523 887 246 3079 Fragments arranged by size: 3079 988 887 523 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KasI G'GCGC_C Cuts at: 2865 2865 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3253 3603 3253 Size: 350 5654 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 5118 5302 1305 Size: 744 3069 184 2007 Fragments arranged by size: 3069 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2382 2594 2600 2666 2905 Size: 54 1425 343 550 212 6 66 239 Cuts at: 2905 10 Size: 3109 Fragments arranged by size: 3109 1425 550 343 239 212 66 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3041 3962 4321 4480 518 Size: 648 1257 618 921 359 159 2042 Fragments arranged by size: 2042 1257 921 648 618 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 2651 2893 3420 4097 5245 5490 1606 Size: 575 470 242 527 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 575 527 470 245 242 NaeI GCC'GGC Cuts at: 3191 3191 Size: 6004 NarI GG'CG_CC Cuts at: 2866 2866 Size: 6004 NcoI C'CATG_G Cuts at: 513 1065 2004 2595 513 Size: 552 939 591 3922 Fragments arranged by size: 3922 939 591 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NgoMIV G'CCGG_C Cuts at: 3189 3189 Size: 6004 NheI G'CTAG_C Cuts at: 1052 2704 1052 Size: 1652 4352 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2235 3362 4907 1132 Size: 97 1006 1127 1545 2229 Fragments arranged by size: 2229 1545 1127 1006 97 PasI CC'CwG_GG Cuts at: 1348 2935 1348 Size: 1587 4417 PciI A'CATG_T Cuts at: 2231 4903 2231 Size: 2672 3332 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2967 2967 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 3050 1613 Size: 1437 4567 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 2892 3285 3356 830 Size: 2062 393 71 3478 Fragments arranged by size: 3478 2062 393 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2651 2893 2651 Size: 242 5762 SacI G_AGCT'C Cuts at: 2932 2932 Size: 6004 SacII CC_GC'GG Cuts at: 2182 2182 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SexAI A'CCwGG_T Cuts at: 2019 2019 Size: 6004 SfcI C'TryA_G Cuts at: 826 1045 2888 3281 3352 4487 5168 5359 826 Size: 219 1843 393 71 1135 681 191 1471 Fragments arranged by size: 1843 1471 1135 681 393 219 191 71 SfoI GGC'GCC Cuts at: 2867 2867 Size: 6004 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 2159 152 Size: 2007 3997 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2249 2402 2861 3212 1517 Size: 732 153 459 351 4309 Fragments arranged by size: 4309 732 459 351 153 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2595 3104 3443 513 Size: 552 939 57 534 509 339 3074 Fragments arranged by size: 3074 939 552 534 509 339 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2255 4250 5962 Size: 274 80 33 51 1443 278 1995 1712 Cuts at: 5962 96 Size: 138 Fragments arranged by size: 1995 1712 1443 278 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3127 3159 5964 5996 3127 Size: 32 2805 32 3135 Fragments arranged by size: 3135 2805 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 2587 2431 Size: 156 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI