################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI ApaLI AscI Asp718I AspEI AspI AvrII BbeI BbrPI BbsI BbvCI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiWI BsmAI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 802 GAATTC [GA]^AATT[CT] AocI 357 CCTGAGG CC^T[ACGT]AGG Aor51HI 614 AGCGCT AGC^GCT ApoI 802 GAATTC [GA]^AATT[CT] AseI 1747 ATTAAT AT^TAAT AsnI 1747 ATTAAT AT^TAAT Asp700I 313 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 665 GAGCAC G[AT]GC[AT]^C BanI 1869 GGCACC G^G[CT][GA]CC BcgI 993 CGACTATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 892 GCCCGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 1253 ACCGGA [AT]^CCGG[AT] BsiEI 1320 CGATCG CG[GA][CT]^CG BsiHKAI 665 GAGCAC G[AT]GC[AT]^C BsmI 161 GCATTC GAATGC(1/-1) Bsp1407I 914 TGTACA T^GTACA BspLU11I 917 ACATGT A^CATGT BsrBI 441 CCGCTC CCGCTC(-3/-3) BsrDI 74 GCAATG GCAATG(2/0) BsrFI 200 ACCGGC [GA]^CCGG[CT] BsrGI 914 TGTACA T^GTACA BstAPI 1327 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1713 CCAGTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 357 CCTGAGG CC^T[ACGT]AGG Cfr10I 200 ACCGGC [GA]^CCGG[CT] DraIII 1007 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1296 CTCTTC CTCTTC(1/4) Eco47III 614 AGCGCT AGC^GCT Eco81I 357 CCTGAGG CC^T[ACGT]AGG EcoRI 802 GAATTC G^AATTC HaeII 614 AGCGCT [GA]GCGC^[CT] HincII 1794 GTTGAC GT[CT]^[GA]AC HindII 1794 GTTGAC GT[CT]^[GA]AC Ksp632I 1296 CTCTTC CTCTTC(1/4) MspA1I 467 CAGCTG C[AC]G^C[GT]G NspI 917 ACATGT [GA]CATG^[CT] PshBI 1747 ATTAAT AT^TAAT PvuI 1320 CGATCG CGAT^CG PvuII 467 CAGCTG CAG^CTG SbfI 696 CCTGCAGG CCTGCA^GG Sse8387I 696 CCTGCAGG CCTGCA^GG SspBI 914 TGTACA T^GTACA SspI 314 AATATT AAT^ATT TaqI 1323 TCGA T^CGA TfiI 2011 GATTC G^A[AT]TC TthHB8I 1323 TCGA T^CGA XcmI 1844 CCATCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 313 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 62 CGCG CG^CG 64 CGCG AflIII 917 ACATGT A^C[GA][CT]GT 1443 ACGTGT AvaI 364 CCCGGG C^[CT]CG[GA]G 1781 CCCGGG AviII 71 TGCGCA TGC^GCA 1559 TGCGCA BamHI 1346 GGATCC G^GATCC 1682 GGATCC BclI 791 TGATCA T^GATCA 1379 TGATCA BfaI 418 CTAG C^TAG 820 CTAG BpmI 1406 CTGGAG CTGGAG(16/14) 1861 CTGGAG BseRI 444 CTCCTC GAGGAG(10/8) 514 CTCCTC BsgI 380 GTGCAG GTGCAG(16/14) 547 GTGCAG BsoBI 364 CCCGGG C^[CT]CG[GA]G 1781 CCCGGG BspHI 264 TCATGA T^CATGA 333 TCATGA BstUI 62 CGCG CG^CG 64 CGCG BstYI 1346 GGATCC [GA]^GATC[CT] 1682 GGATCC DsaI 713 CCACGG C^C[GA][CT]GG 894 CCGTGG EcoT14I 1730 CCAAGG C^C[AT][AT]GG 1761 CCTTGG FauI 182 CCCGC CCCGC(4/6) 1995 CCCGC FbaI 791 TGATCA T^GATCA 1379 TGATCA FspI 71 TGCGCA TGC^GCA 1559 TGCGCA MaeI 418 CTAG C^TAG 820 CTAG MflI 1346 GGATCC [GA]^GATC[CT] 1682 GGATCC MvnI 62 CGCG CG^CG 64 CGCG NdeI 1118 CATATG CA^TATG 1328 CATATG RcaI 264 TCATGA T^CATGA 333 TCATGA SmaI 364 CCCGGG CCC^GGG 1781 CCCGGG StyI 1730 CCAAGG C^C[AT][AT]GG 1761 CCTTGG XhoII 1346 GGATCC [GA]^GATC[CT] 1682 GGATCC XmaCI 364 CCCGGG C^CCGGG 1781 CCCGGG XmaI 364 CCCGGG C^CCGGG 1781 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI ApaI AvaII BalI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I Tsp509I TspRI Van91I