(Circular) (Six-base) MAPSORT of: pFN21AE4556.seq Check: 9677 from: 1 to: 6736 pFN21AE4556 6736 bp With 182 enzymes: SgfI * March 28, 2012 14:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4546 278 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4079 1937 Size: 2142 4594 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 4863 5236 1563 Size: 3300 373 3063 Fragments arranged by size: 3300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 4799 6183 6451 1632 Size: 602 2565 1384 268 1917 Fragments arranged by size: 2565 1917 1384 602 268 AfeI AGC'GCT Cuts at: 2621 2621 Size: 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 2922 3448 5635 6612 1203 Size: 1719 526 2187 977 1327 Fragments arranged by size: 2187 1719 1327 977 526 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 5182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6736 AlwNI CAG_nnn'CTG Cuts at: 4479 6051 4479 Size: 1572 5164 ApaI G_GGCC'C Cuts at: 2063 2376 2701 3195 3625 3794 2063 Size: 313 325 494 430 169 5005 Fragments arranged by size: 5005 494 430 325 313 169 ApaLI G'TGCA_C Cuts at: 4793 5949 4793 Size: 1156 5580 ApoI r'AATT_y Cuts at: 1493 2807 4045 4271 4423 6532 1493 Size: 1314 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3753 5290 160 Size: 872 2721 1537 1606 Fragments arranged by size: 2721 1606 1537 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 2369 3786 4061 1870 Size: 73 426 1417 275 4545 Fragments arranged by size: 4545 1417 426 275 73 BamHI G'GATC_C Cuts at: 3351 3687 4066 4433 3351 Size: 336 379 367 5654 Fragments arranged by size: 5654 379 367 336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3874 4057 5512 618 Size: 325 217 627 2087 183 1455 1842 Fragments arranged by size: 2087 1842 1455 627 325 217 183 BanII G_rGCy'C Cuts at: 1391 2063 2277 2376 2701 2784 3195 3625 3794 Size: 672 214 99 325 83 411 430 169 Cuts at: 3794 4055 1391 Size: 261 4072 Fragments arranged by size: 4072 672 430 411 325 261 214 169 99 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5765 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2987 3021 4927 4961 1903 Size: 34 50 34 966 34 1906 34 3678 Fragments arranged by size: 3678 1906 966 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4628 5838 4628 Size: 1210 5526 BclI T'GATC_A Cuts at: 1548 2796 3384 1548 Size: 1248 588 4900 Fragments arranged by size: 4900 1248 588 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 5347 136 Size: 107 122 71 631 605 1231 2444 1525 Fragments arranged by size: 2444 1525 1231 631 605 122 107 71 BglII A'GATC_T Cuts at: 6731 6731 Size: 6736 BlpI GC'TnA_GC Cuts at: 4153 4153 Size: 6736 Bme1580I G_kGCm'C Cuts at: 1790 2063 2187 2259 2376 2701 3195 3625 3794 Size: 273 124 72 117 325 494 430 169 Cuts at: 3794 3816 3877 4797 5953 1790 Size: 22 61 920 1156 2573 Fragments arranged by size: 2573 1156 920 494 430 325 273 169 124 117 72 61 22 BmgBI CAC'GTC Cuts at: 1318 3449 1318 Size: 2131 4605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2842 3675 4467 Size: 416 53 83 399 24 1425 833 792 Cuts at: 4467 5425 442 Size: 958 1753 Fragments arranged by size: 1753 1425 958 833 792 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3432 3887 5396 1239 Size: 2193 455 1509 2579 Fragments arranged by size: 2579 2193 1509 455 Bpu10I CC'TnA_GC Cuts at: 2762 2762 Size: 6736 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 3258 3389 4861 5726 6024 2114 Size: 1144 131 1472 865 298 2826 Fragments arranged by size: 2826 1472 1144 865 298 131 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5399 882 Size: 617 3900 2219 Fragments arranged by size: 3900 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4527 493 Size: 711 3323 2702 Fragments arranged by size: 3323 2702 711 BsaBI GATnn'nnATC Cuts at: 6730 6730 Size: 6736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4543 4925 5560 275 Size: 53 83 186 936 3010 382 635 1451 Fragments arranged by size: 3010 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3258 5169 5567 5841 5988 6399 3258 Size: 1911 398 274 147 411 3595 Fragments arranged by size: 3595 1911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2526 2556 1178 Size: 30 165 30 1123 30 5358 Fragments arranged by size: 5358 1123 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2440 2510 1186 Size: 1254 70 5412 Fragments arranged by size: 5412 1254 70 BseYI C'CCAG_C Cuts at: 2228 2344 3023 3289 3582 3816 5939 6435 2228 Size: 116 679 266 293 234 2123 496 2529 Fragments arranged by size: 2529 2123 679 496 293 266 234 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2406 2573 1163 Size: 573 670 167 5326 Fragments arranged by size: 5326 670 573 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4186 4947 5975 1540 Size: 275 186 1327 858 761 1028 2301 Fragments arranged by size: 2301 1327 1028 858 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2674 4797 4882 5953 2674 Size: 2123 85 1071 3457 Fragments arranged by size: 3457 2123 1071 85 BsmI GAATG_Cn' Cuts at: 2166 4247 4340 2166 Size: 2081 93 4562 Fragments arranged by size: 4562 2081 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2187 2259 2277 2376 2674 2701 Size: 399 273 124 72 18 99 298 27 Cuts at: 2701 2784 3195 3625 3794 3816 3877 4055 4797 Size: 83 411 430 169 22 61 178 742 Cuts at: 4797 4882 5953 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 411 399 298 273 178 169 124 99 85 83 72 61 27 22 18 BspEI T'CCGG_A Cuts at: 6399 6399 Size: 6736 BspHI T'CATG_A Cuts at: 2269 2338 4625 6355 2269 Size: 69 2287 1730 2650 Fragments arranged by size: 2650 2287 1730 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2419 2545 2610 4091 844 Size: 1575 126 65 1481 3489 Fragments arranged by size: 3489 1575 1481 126 65 BsrBI CCG'CTC Cuts at: 1394 1823 2448 4623 1394 Size: 429 625 2175 3507 Fragments arranged by size: 3507 2175 625 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2086 5231 5405 65 Size: 1183 838 3145 174 1396 Fragments arranged by size: 3145 1396 1183 838 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 5380 5567 1700 Size: 110 395 3175 187 2869 Fragments arranged by size: 3175 2869 395 187 110 BsrGI T'GTAC_A Cuts at: 96 2919 96 Size: 2823 3913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 4796 5808 4796 Size: 1012 5724 BstAPI GCAn_nnn'nTGC Cuts at: 794 3338 794 Size: 2544 4192 BstBI TT'CG_AA Cuts at: 5540 5540 Size: 6736 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6736 BstXI CCAn_nnnn'nTGG Cuts at: 3725 3725 Size: 6736 BstYI r'GATC_y Cuts at: 1972 3351 3687 4066 4433 4818 4835 6287 6373 Size: 1379 336 379 367 385 17 1452 86 Cuts at: 6373 6385 6731 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1379 385 379 367 346 336 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2363 2363 Size: 6736 BtgI C'CryG_G Cuts at: 513 1065 2718 2899 513 Size: 552 1653 181 4350 Fragments arranged by size: 4350 1653 552 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5997 Fragments arranged by size: 5997 683 56 BtsI GCAGTG_nn' Cuts at: 2874 3567 3892 4252 5046 5066 2874 Size: 693 325 360 794 20 4544 Fragments arranged by size: 4544 794 693 360 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 4040 4040 Size: 6736 DraIII CAC_nnn'GTG Cuts at: 3017 3017 Size: 6736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5743 809 Size: 507 4427 1802 Fragments arranged by size: 4427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2279 3601 Size: 54 1425 50 275 18 307 142 1322 Cuts at: 3601 3769 3779 4183 5072 6701 8 Size: 168 10 404 889 1629 43 Fragments arranged by size: 1629 1425 1322 889 404 307 275 168 142 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 4183 1537 Size: 275 2371 4090 Fragments arranged by size: 4090 2371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3307 4666 1353 Size: 1954 1359 3423 Fragments arranged by size: 3423 1954 1359 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5323 5697 5843 387 Size: 1064 138 3734 374 146 1280 Fragments arranged by size: 3734 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 3432 3887 4799 5396 6183 6451 Size: 393 602 1198 455 912 597 787 268 Cuts at: 6451 1239 Size: 1524 Fragments arranged by size: 1524 1198 912 787 602 597 455 393 268 EcoO109I rG'GnC_Cy Cuts at: 2060 2373 2697 2698 2707 2789 3028 3092 3790 Size: 313 324 1 9 82 239 64 698 Cuts at: 3790 3791 2060 Size: 1 5005 Fragments arranged by size: 5005 698 324 313 239 82 64 9 1 1 EcoRI G'AATT_C Cuts at: 2807 4045 4423 2807 Size: 1238 378 5120 Fragments arranged by size: 5120 1238 378 FspI TGC'GCA Cuts at: 2078 3566 5242 2078 Size: 1488 1676 3572 Fragments arranged by size: 3572 1676 1488 HaeII r_GCGC'y Cuts at: 1678 2623 5883 1678 Size: 945 3260 2531 Fragments arranged by size: 3260 2531 945 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 2500 2532 2781 Size: 32 135 32 845 32 51 32 249 Cuts at: 2781 2813 3940 3947 3972 3979 5377 5409 5451 Size: 32 1127 7 25 7 1398 32 42 Cuts at: 5451 5483 1373 Size: 32 2626 Fragments arranged by size: 2626 1398 1127 845 249 135 51 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3801 4080 4326 669 Size: 988 281 1863 279 246 3079 Fragments arranged by size: 3079 1863 988 281 279 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6736 HpaI GTT'AAC Cuts at: 4326 4326 Size: 6736 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 4335 4335 Size: 6736 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 3660 5850 6034 1305 Size: 1173 1182 2190 184 2007 Fragments arranged by size: 2190 2007 1182 1173 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 2281 3603 3781 10 Size: 54 1425 343 307 142 1322 178 2965 Fragments arranged by size: 2965 1425 1322 343 307 178 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3609 3827 3854 4694 5053 5212 Size: 648 1554 889 218 27 840 359 159 Cuts at: 5212 518 Size: 2042 Fragments arranged by size: 2042 1554 889 840 648 359 218 159 27 MspA1I CmG'CkG Cuts at: 1606 2474 4152 4829 5977 6222 1606 Size: 868 1678 677 1148 245 2120 Fragments arranged by size: 2120 1678 1148 868 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6184 NdeI CA'TA_TG Cuts at: 387 3124 3334 387 Size: 2737 210 3789 Fragments arranged by size: 3789 2737 210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NspI r_CATG'y Cuts at: 1132 1229 2926 4094 5639 1132 Size: 97 1697 1168 1545 2229 Fragments arranged by size: 2229 1697 1545 1168 97 PasI CC'CwG_GG Cuts at: 1348 3358 1348 Size: 2010 4726 PciI A'CATG_T Cuts at: 2922 5635 2922 Size: 2713 4023 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 2635 3935 1108 Size: 1206 321 1300 3909 Fragments arranged by size: 3909 1300 1206 321 PfoI T'CCnGG_A Cuts at: 3724 3724 Size: 6736 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2789 3028 3092 2789 Size: 239 64 6433 Fragments arranged by size: 6433 239 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6736 PsiI TTA'TAA Cuts at: 2546 4306 2546 Size: 1760 4976 PspOMI G'GGCC_C Cuts at: 2059 2372 2697 3191 3621 3790 2059 Size: 313 325 494 430 169 5005 Fragments arranged by size: 5005 494 430 325 313 169 PstI C_TGCA'G Cuts at: 830 2616 2706 3884 4088 830 Size: 1786 90 1178 204 3478 Fragments arranged by size: 3478 1786 1178 204 90 PvuI CG_AT'CG Cuts at: 2001 3328 2001 Size: 1327 5409 PvuII CAG'CTG Cuts at: 2474 2474 Size: 6736 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SbfI CC_TGCA'GG Cuts at: 2706 4088 2706 Size: 1382 5354 ScaI AGT'ACT Cuts at: 4984 6696 4984 Size: 1712 5024 SfcI C'TryA_G Cuts at: 826 1045 2110 2612 2702 3880 4084 5219 5900 Size: 219 1065 502 90 1178 204 1135 681 Cuts at: 5900 6091 826 Size: 191 1471 Fragments arranged by size: 1471 1178 1135 1065 681 502 219 204 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 2371 3788 4063 1872 Size: 499 1417 275 4545 Fragments arranged by size: 4545 1417 499 275 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 3273 3368 4840 5741 6003 Size: 197 926 186 1144 95 1472 901 262 Cuts at: 6003 820 Size: 1553 Fragments arranged by size: 1553 1472 1144 926 901 262 197 186 95 SnaBI TAC'GTA Cuts at: 493 4527 493 Size: 4034 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6736 SphI G_CATG'C Cuts at: 1132 4094 1132 Size: 2962 3774 SrfI GCCC'GGGC Cuts at: 2371 2371 Size: 6736 SspI AAT'ATT Cuts at: 5 52 2321 4660 5 Size: 47 2269 2339 2081 Fragments arranged by size: 2339 2269 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6736 StyI C'CwwG_G Cuts at: 513 1065 3735 3766 4175 513 Size: 552 2670 31 409 3074 Fragments arranged by size: 3074 2670 552 409 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 2876 4763 4780 4933 5118 5563 Size: 1255 522 774 1887 17 153 185 445 Cuts at: 5563 325 Size: 1498 Fragments arranged by size: 1887 1498 1255 774 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2919 4982 6694 Size: 274 80 33 51 1443 942 2063 1712 Cuts at: 6694 96 Size: 138 Fragments arranged by size: 2063 1712 1443 942 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3164 3196 3617 3649 3854 3886 6696 6728 3164 Size: 32 421 32 205 32 2810 32 3172 Fragments arranged by size: 3172 2810 421 205 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 4072 4072 Size: 6736 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3856 3856 Size: 6736 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 2369 3786 4061 1870 Size: 499 1417 275 4545 Fragments arranged by size: 4545 1417 499 275 XmnI GAAnn'nnTTC Cuts at: 2322 4865 2322 Size: 2543 4193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4544 276 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI