(Linear) (Six-base) MAPSORT of: KIEE4556.seq Check: 9333 from: 1 to: 2031 KIEE4556.orf With 182 enzymes: SgfI * March 22, 2012 18:22 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 2031 Size: 229 1802 AfeI AGC'GCT Cuts at: 0 616 2031 Size: 616 1415 AflIII A'CryG_T Cuts at: 0 917 1443 2031 Size: 917 526 588 Fragments arranged by size: 917 588 526 AleI CACnn'nnGTG Cuts at: 0 715 2031 Size: 715 1316 ApaI G_GGCC'C Cuts at: 0 58 371 696 1190 1620 1789 2031 Size: 58 313 325 494 430 169 242 Fragments arranged by size: 494 430 325 313 242 169 58 ApoI r'AATT_y Cuts at: 0 802 2031 Size: 802 1229 AseI AT'TA_AT Cuts at: 0 1748 2031 Size: 1748 283 AvaI C'yCGr_G Cuts at: 0 364 1781 2031 Size: 364 1417 250 Fragments arranged by size: 1417 364 250 BamHI G'GATC_C Cuts at: 0 1346 1682 2031 Size: 1346 336 349 Fragments arranged by size: 1346 349 336 BanI G'GyrC_C Cuts at: 0 1869 2031 Size: 1869 162 BanII G_rGCy'C Cuts at: 0 58 272 371 696 779 1190 1620 1789 Size: 58 214 99 325 83 411 430 169 Cuts at: 1789 2031 Size: 242 Fragments arranged by size: 430 411 325 242 214 169 99 83 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 982 1016 2031 Size: 982 34 1015 Fragments arranged by size: 1015 982 34 BclI T'GATC_A Cuts at: 0 791 1379 2031 Size: 791 588 652 Fragments arranged by size: 791 652 588 BglI GCCn_nnn'nGGC Cuts at: 0 898 2031 Size: 898 1133 Bme1580I G_kGCm'C Cuts at: 0 58 182 254 371 696 1190 1620 1789 Size: 58 124 72 117 325 494 430 169 Cuts at: 1789 1811 1872 2031 Size: 22 61 159 Fragments arranged by size: 494 430 325 169 159 124 117 72 61 58 22 BmgBI CAC'GTC Cuts at: 0 1444 2031 Size: 1444 587 BmrI ACTGGGnnnn_n' Cuts at: 0 837 1670 2031 Size: 837 833 361 Fragments arranged by size: 837 833 361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1427 1882 2031 Size: 1427 455 149 Fragments arranged by size: 1427 455 149 Bpu10I CC'TnA_GC Cuts at: 0 757 2031 Size: 757 1274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1253 1384 2031 Size: 109 1144 131 647 Fragments arranged by size: 1144 647 131 109 BsaWI w'CCGG_w Cuts at: 0 1253 2031 Size: 1253 778 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 521 551 2031 Size: 521 30 1480 Fragments arranged by size: 1480 521 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 435 505 2031 Size: 435 70 1526 Fragments arranged by size: 1526 435 70 BseYI C'CCAG_C Cuts at: 0 223 339 1018 1284 1577 1811 2031 Size: 223 116 679 266 293 234 220 Fragments arranged by size: 679 293 266 234 223 220 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 568 2031 Size: 401 167 1463 Fragments arranged by size: 1463 401 167 BsiEI CG_ry'CG Cuts at: 0 1323 2031 Size: 1323 708 BsiHKAI G_wGCw'C Cuts at: 0 669 2031 Size: 669 1362 BsmI GAATG_Cn' Cuts at: 0 161 2031 Size: 161 1870 Bsp1286I G_dGCh'C Cuts at: 0 58 182 254 272 371 669 696 779 Size: 58 124 72 18 99 298 27 83 Cuts at: 779 1190 1620 1789 1811 1872 2031 Size: 411 430 169 22 61 159 Fragments arranged by size: 430 411 298 169 159 124 99 83 72 61 58 27 22 18 BspHI T'CATG_A Cuts at: 0 264 333 2031 Size: 264 69 1698 Fragments arranged by size: 1698 264 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 414 540 605 2031 Size: 414 126 65 1426 Fragments arranged by size: 1426 414 126 65 BsrBI CCG'CTC Cuts at: 0 443 2031 Size: 443 1588 BsrDI GCAATG_nn' Cuts at: 0 81 2031 Size: 81 1950 BsrFI r'CCGG_y Cuts at: 0 200 2031 Size: 200 1831 BsrGI T'GTAC_A Cuts at: 0 914 2031 Size: 914 1117 BstAPI GCAn_nnn'nTGC Cuts at: 0 1333 2031 Size: 1333 698 BstXI CCAn_nnnn'nTGG Cuts at: 0 1720 2031 Size: 1720 311 BstYI r'GATC_y Cuts at: 0 1346 1682 2031 Size: 1346 336 349 Fragments arranged by size: 1346 349 336 Bsu36I CC'TnA_GG Cuts at: 0 358 2031 Size: 358 1673 BtgI C'CryG_G Cuts at: 0 713 894 2031 Size: 713 181 1137 Fragments arranged by size: 1137 713 181 BtsI GCAGTG_nn' Cuts at: 0 869 1562 1887 2031 Size: 869 693 325 144 Fragments arranged by size: 869 693 325 144 DraIII CAC_nnn'GTG Cuts at: 0 1012 2031 Size: 1012 1019 EaeI y'GGCC_r Cuts at: 0 132 274 1596 1764 1774 2031 Size: 132 142 1322 168 10 257 Fragments arranged by size: 1322 257 168 142 132 10 EarI CTCTTCn'nnn_ Cuts at: 0 1302 2031 Size: 1302 729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 1427 1882 2031 Size: 229 1198 455 149 Fragments arranged by size: 1198 455 229 149 EcoO109I rG'GnC_Cy Cuts at: 0 55 368 692 693 702 784 1023 1087 Size: 55 313 324 1 9 82 239 64 Cuts at: 1087 1785 1786 2031 Size: 698 1 245 Fragments arranged by size: 698 324 313 245 239 82 64 55 9 1 1 EcoRI G'AATT_C Cuts at: 0 802 2031 Size: 802 1229 FspI TGC'GCA Cuts at: 0 73 1561 2031 Size: 73 1488 470 Fragments arranged by size: 1488 470 73 HaeII r_GCGC'y Cuts at: 0 618 2031 Size: 618 1413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 495 527 776 808 1935 1942 Size: 412 32 51 32 249 32 1127 7 Cuts at: 1942 1967 1974 2031 Size: 25 7 57 Fragments arranged by size: 1127 412 249 57 51 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 0 1796 2031 Size: 1796 235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 473 1655 2031 Size: 473 1182 376 Fragments arranged by size: 1182 473 376 MscI TGG'CCA Cuts at: 0 134 276 1598 1776 2031 Size: 134 142 1322 178 255 Fragments arranged by size: 1322 255 178 142 134 MslI CAynn'nnrTG Cuts at: 0 715 1604 1822 1849 2031 Size: 715 889 218 27 182 Fragments arranged by size: 889 715 218 182 27 MspA1I CmG'CkG Cuts at: 0 469 2031 Size: 469 1562 NdeI CA'TA_TG Cuts at: 0 1119 1329 2031 Size: 1119 210 702 Fragments arranged by size: 1119 702 210 NspI r_CATG'y Cuts at: 0 921 2031 Size: 921 1110 PasI CC'CwG_GG Cuts at: 0 1353 2031 Size: 1353 678 PciI A'CATG_T Cuts at: 0 917 2031 Size: 917 1114 PflMI CCAn_nnn'nTGG Cuts at: 0 309 630 1930 2031 Size: 309 321 1300 101 Fragments arranged by size: 1300 321 309 101 PfoI T'CCnGG_A Cuts at: 0 1719 2031 Size: 1719 312 PpuMI rG'GwC_Cy Cuts at: 0 784 1023 1087 2031 Size: 784 239 64 944 Fragments arranged by size: 944 784 239 64 PsiI TTA'TAA Cuts at: 0 541 2031 Size: 541 1490 PspOMI G'GGCC_C Cuts at: 0 54 367 692 1186 1616 1785 2031 Size: 54 313 325 494 430 169 246 Fragments arranged by size: 494 430 325 313 246 169 54 PstI C_TGCA'G Cuts at: 0 611 701 1879 2031 Size: 611 90 1178 152 Fragments arranged by size: 1178 611 152 90 PvuI CG_AT'CG Cuts at: 0 1323 2031 Size: 1323 708 PvuII CAG'CTG Cuts at: 0 469 2031 Size: 469 1562 SbfI CC_TGCA'GG Cuts at: 0 701 2031 Size: 701 1330 SfcI C'TryA_G Cuts at: 0 105 607 697 1875 2031 Size: 105 502 90 1178 156 Fragments arranged by size: 1178 502 156 105 90 SmaI CCC'GGG Cuts at: 0 366 1783 2031 Size: 366 1417 248 Fragments arranged by size: 1417 366 248 SmlI C'TyrA_G Cuts at: 0 124 1268 1363 2031 Size: 124 1144 95 668 Fragments arranged by size: 1144 668 124 95 SrfI GCCC'GGGC Cuts at: 0 366 2031 Size: 366 1665 SspI AAT'ATT Cuts at: 0 316 2031 Size: 316 1715 StyI C'CwwG_G Cuts at: 0 1730 1761 2031 Size: 1730 31 270 Fragments arranged by size: 1730 270 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 97 871 2031 Size: 97 774 1160 Fragments arranged by size: 1160 774 97 TatI w'GTAC_w Cuts at: 0 914 2031 Size: 914 1117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1612 1644 1849 1881 2031 Size: 1159 32 421 32 205 32 150 Fragments arranged by size: 1159 421 205 150 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1851 2031 Size: 1851 180 XmaI C'CCGG_G Cuts at: 0 364 1781 2031 Size: 364 1417 250 Fragments arranged by size: 1417 364 250 XmnI GAAnn'nnTTC Cuts at: 0 317 2031 Size: 317 1714 Enzymes that do cut: AcuI AfeI AflIII AleI ApaI ApoI AseI AvaI BamHI BanI BanII BcgI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI NspI PasI PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuI PvuII SbfI SfcI SmaI SmlI SrfI SspI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BfrBI BglII BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI BsiWI BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PspXI PsrI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI StuI SwaI Tth111I XbaI XhoI ZraI