Restriction map for
pFN21AB3630
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 97 GTCTAC GT^[AC][GT]AC
AccIII 90 TCCGGA T^CCGGA
AocI 360 CCTGAGG CC^T[ACGT]AGG
AvaI 524 CTCGGG C^[CT]CG[GA]G
BanI 40 GGTGCC G^G[CT][GA]CC
BanII 409 GAGCCC G[GA]GC[CT]^C
BbvI 4 GCTGC GCAGC(8/12)
BglI 520 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 476 GCTCAGC GC^T[ACGT]AGC
BpmI 535 CTCCAG CTGGAG(16/14)
Bpu1102I 476 GCTCAGC GC^T[ACGT]AGC
BsaJI 488 CCAAGG C^C[ACGT][ACGT]GG
BseAI 90 TCCGGA T^CCGGA
BseRI 61 CTCCTC GAGGAG(10/8)
BsiYI 432 CCGGGACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 432 CCGGGACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 524 CTCGGG C^[CT]CG[GA]G
BspEI 90 TCCGGA T^CCGGA
BstXI 332 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 360 CCTGAGG CC^T[ACGT]AGG
CelII 476 GCTCAGC GC^T[ACGT]AGC
DraII 356 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 226 GACACTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 360 CCTGAGG CC^T[ACGT]AGG
EcoO109I 356 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 488 CCAAGG C^C[AT][AT]GG
FauI 501 GCGGG CCCGC(4/6)
HgaI 555 GCGTC GACGC(5/10)
MroI 90 TCCGGA T^CCGGA
PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT]
SmlI 469 CTTGAG C^T[CT][GA]AG
StyI 488 CCAAGG C^C[AT][AT]GG
TseI 4 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 81 GGATC GGATC(4/5)
449 GGATC
Asp700I 277 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
309 GAAGCACTTC
AspHI 373 GAGCAC G[AT]GC[AT]^C
474 GTGCTC
BalI 392 TGGCCA TGG^CCA
518 TGGCCA
BcnI 222 CCCGG CC^[GC]GG
432 CCGGG
BsaWI 90 TCCGGA [AT]^CCGG[AT]
446 ACCGGA
BsiHKAI 373 GAGCAC G[AT]GC[AT]^C
474 GTGCTC
BspMI 55 ACCTGC ACCTGC(4/8)
153 GCAGGT
EaeI 392 TGGCCA [CT]^GGCC[GA]
518 TGGCCA
Fnu4HI 4 GCTGC GC^[ACGT]GC
207 GCGGC
HphI 24 GGTGA GGTGA(8/7)
306 GGTGA
ItaI 4 GCTGC GC^[ACGT]GC
207 GCGGC
MaeII 101 ACGT A^CGT
326 ACGT
MluNI 392 TGGCCA TGG^CCA
518 TGGCCA
MscI 392 TGGCCA TGG^CCA
518 TGGCCA
NciI 222 CCCGG CC^[GC]GG
432 CCGGG
NlaIII 243 CATG CATG^
438 CATG
TaqI 201 TCGA T^CGA
257 TCGA
TspRI 228 CACTG CA[GC]TG[ACGT][ACGT]^
453 CAGTG
TthHB8I 201 TCGA T^CGA
257 TCGA
XcmI 332 CCATCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
380 CCAGGAGAGAGCTGG
XmnI 277 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
309 GAAGCACTTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
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AciI AluI AvaII BbsI BmyI
BpuAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FokI HaeIII HapII HpaII
MboI MboII MnlI MslI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI