Restriction map for
pFN21AB5684
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbvCI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 462 CGCG CG^CG
Alw44I 441 GTGCAC G^TGCAC
AlwI 726 GATCC GGATC(4/5)
ApaI 1498 GGGCCC GGGCC^C
ApaLI 441 GTGCAC G^TGCAC
AvaI 1626 CCCGGG C^[CT]CG[GA]G
BbeI 433 GGCGCC GGCGC^C
BbsI 52 GTCTTC GAAGAC(2/6)
BfaI 1657 CTAG C^TAG
BglII 103 AGATCT A^GATCT
BpmI 1478 CTGGAG CTGGAG(16/14)
BpuAI 52 GTCTTC GAAGAC(2/6)
BsgI 1425 CTGCAC GTGCAG(16/14)
BsiEI 1681 CGACCG CG[GA][CT]^CG
BsoBI 1626 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1634 TGTACA T^GTACA
BsrGI 1634 TGTACA T^GTACA
BstAPI 1280 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1644 GGTTACC G^GT[ACGT]ACC
BstPI 1644 GGTTACC G^GT[ACGT]ACC
BstUI 462 CGCG CG^CG
BstXI 503 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 103 AGATCT [GA]^GATC[CT]
EaeI 1566 TGGCCG [CT]^GGCC[GA]
EcoO65I 1644 GGTTACC G^GT[ACGT]ACC
HaeII 433 GGCGCC [GA]GCGC^[CT]
HgaI 1519 GACGC GACGC(5/10)
KasI 433 GGCGCC G^GCGCC
MaeI 1657 CTAG C^TAG
MflI 103 AGATCT [GA]^GATC[CT]
MvnI 462 CGCG CG^CG
NarI 433 GGCGCC GG^CGCC
SacI 1333 GAGCTC GAGCT^C
SapI 942 GCTCTTC GCTCTTC(1/4)
ScaI 866 AGTACT AGT^ACT
SfoI 433 GGCGCC GGC^GCC
SmaI 1626 CCCGGG CCC^GGG
SspBI 1634 TGTACA T^GTACA
SspI 200 AATATT AAT^ATT
StuI 1358 AGGCCT AGG^CCT
XhoII 103 AGATCT [GA]^GATC[CT]
XmaCI 1626 CCCGGG C^CCGGG
XmaI 1626 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 433 GGCGCC G[GA]^CG[CT]C
1519 GACGCC
BcgI 333 CGAAGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
690 CGAGTCGGGTGC
BsaHI 433 GGCGCC G[GA]^CG[CT]C
1519 GACGCC
BsrDI 1218 CATTGC GCAATG(2/0)
7 GCAATG
DraIII 235 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1281 CACTGTGTG
DsaI 669 CCGTGG C^C[GA][CT]GG
712 CCGTGG
EcoRI 717 GAATTC G^AATTC
1189 GAATTC
EcoT14I 265 CCAAGG C^C[AT][AT]GG
1640 CCAAGG
Hin1I 433 GGCGCC G[GA]^CG[CT]C
1519 GACGCC
MseI 578 TTAA T^TAA
1445 TTAA
NspI 1466 ACATGC [GA]CATG^[CT]
1676 ACATGC
PflMI 768 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAAGCCCTGG
PleI 553 GACTC GAGTC(4/5)
691 GAGTC
StyI 265 CCAAGG C^C[AT][AT]GG
1640 CCAAGG
Tru9I 578 TTAA T^TAA
1445 TTAA
Van91I 768 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BanI BanII
BbrPI BbvI BcnI BmyI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I