(Circular) (Six-base) MAPSORT of: pFN21AB5684.seq Check: 8961 from: 1 to: 6391 pFN21AB5684 6391 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2300 2906 2300 Size: 606 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3734 1937 Size: 1797 4594 Acc65I G'GTAC_C Cuts at: 3712 3712 Size: 6391 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 3413 4454 5838 6106 1632 Size: 1235 546 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1235 1041 546 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 5290 6267 1203 Size: 4087 977 1327 Fragments arranged by size: 4087 1327 977 AgeI A'CCGG_T Cuts at: 3688 5222 3688 Size: 1534 4857 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6391 AlwNI CAG_nnn'CTG Cuts at: 2249 2305 2646 2779 3051 3429 4134 5706 2249 Size: 56 341 133 272 378 705 1572 2934 Fragments arranged by size: 2934 1572 705 378 341 272 133 56 ApaI G_GGCC'C Cuts at: 3507 3507 Size: 6391 ApaLI G'TGCA_C Cuts at: 2446 4448 5604 2446 Size: 2002 1156 3233 Fragments arranged by size: 3233 2002 1156 ApoI r'AATT_y Cuts at: 1493 2722 3161 3194 3700 3926 4078 6187 1493 Size: 1229 439 33 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 506 439 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3631 3716 1870 Size: 73 1688 85 4545 Fragments arranged by size: 4545 1688 85 73 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2288 2438 2702 2835 3712 Size: 325 217 627 501 150 264 133 877 Cuts at: 3712 5167 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 877 627 501 325 264 217 150 133 BanII G_rGCy'C Cuts at: 1391 2028 2949 3342 3507 3710 1391 Size: 637 921 393 165 203 4072 Fragments arranged by size: 4072 921 637 393 203 165 BbeI G_GCGC'C Cuts at: 2442 2442 Size: 6391 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 928 Size: 971 151 5269 Fragments arranged by size: 5269 971 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2327 2361 2684 2718 4582 4616 1903 Size: 34 390 34 323 34 1864 34 3678 Fragments arranged by size: 3678 1864 390 323 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4283 5493 4283 Size: 1210 5181 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5002 136 Size: 107 122 71 631 605 3330 1525 Fragments arranged by size: 3330 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2108 6386 2108 Size: 4278 2113 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 2233 2291 2336 2417 2450 2773 2886 3265 Size: 443 58 45 81 33 323 113 379 Cuts at: 3265 3507 4452 5608 1790 Size: 242 945 1156 2573 Fragments arranged by size: 2573 1156 945 443 379 323 242 113 81 58 45 33 BmgBI CAC'GTC Cuts at: 1318 2865 1318 Size: 1547 4844 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 2789 4122 Size: 416 53 83 399 24 1343 29 1333 Cuts at: 4122 5080 442 Size: 958 1753 Fragments arranged by size: 1753 1343 1333 958 416 399 83 53 29 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3504 5051 1239 Size: 2265 1547 2579 Fragments arranged by size: 2579 2265 1547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5054 882 Size: 617 3555 2219 Fragments arranged by size: 3555 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2382 2418 2842 4182 493 Size: 711 1178 36 424 1340 2702 Fragments arranged by size: 2702 1340 1178 711 424 36 BsaBI GATnn'nnATC Cuts at: 6385 6385 Size: 6391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2439 3525 4198 4580 Size: 53 83 186 936 906 1086 673 382 Cuts at: 4580 5215 275 Size: 635 1451 Fragments arranged by size: 1451 1086 936 906 673 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3688 4824 5222 5496 5643 6054 3688 Size: 1136 398 274 147 411 4025 Fragments arranged by size: 4025 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2948 2978 1178 Size: 30 165 30 1545 30 4591 Fragments arranged by size: 4591 1545 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 3164 3167 1186 Size: 929 1049 3 4410 Fragments arranged by size: 4410 1049 929 3 BseYI C'CCAG_C Cuts at: 2069 2348 2515 3600 5594 6090 2069 Size: 279 167 1085 1994 496 2370 Fragments arranged by size: 2370 1994 1085 496 279 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3415 1163 Size: 573 1679 4139 Fragments arranged by size: 4139 1679 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3689 3841 4602 5630 1540 Size: 275 186 1688 152 761 1028 2301 Fragments arranged by size: 2301 1688 1028 761 275 186 152 BsiHKAI G_wGCw'C Cuts at: 2187 2426 2450 3342 4452 4537 5608 2187 Size: 239 24 892 1110 85 1071 2970 Fragments arranged by size: 2970 1110 1071 892 239 85 24 BsmI GAATG_Cn' Cuts at: 3902 3995 3902 Size: 93 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2187 2233 2291 2336 2417 2426 Size: 399 238 159 46 58 45 81 9 Cuts at: 2426 2450 2773 2886 2949 3265 3342 3507 3710 Size: 24 323 113 63 316 77 165 203 Cuts at: 3710 4452 4537 5608 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 323 316 238 203 165 159 113 85 81 77 63 58 46 45 24 9 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2300 2906 3318 3746 844 Size: 1456 606 412 428 3489 Fragments arranged by size: 3489 1456 606 428 412 BsrBI CCG'CTC Cuts at: 1394 1823 3019 3388 3498 4278 1394 Size: 429 1196 369 110 780 3507 Fragments arranged by size: 3507 1196 780 429 369 110 BsrDI GCAATG_nn' Cuts at: 65 1248 2019 3222 4886 5060 65 Size: 1183 771 1203 1664 174 1396 Fragments arranged by size: 1664 1396 1203 1183 771 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3688 5035 5222 1700 Size: 110 1878 1347 187 2869 Fragments arranged by size: 2869 1878 1347 187 110 BsrGI T'GTAC_A Cuts at: 96 3639 96 Size: 3543 2848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 3291 794 Size: 2497 3894 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 3649 1184 Size: 2465 3926 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 6391 BstYI r'GATC_y Cuts at: 1972 2108 3721 4088 4473 4490 5942 6028 6040 Size: 136 1613 367 385 17 1452 86 12 Cuts at: 6040 6386 1972 Size: 346 1977 Fragments arranged by size: 1977 1613 1452 385 367 346 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2674 2717 513 Size: 552 939 670 43 4187 Fragments arranged by size: 4187 939 670 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2944 487 Size: 683 56 1718 3934 Fragments arranged by size: 3934 1718 683 56 BtsI GCAGTG_nn' Cuts at: 3073 3907 4701 4721 3073 Size: 834 794 20 4743 Fragments arranged by size: 4743 834 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DraIII CAC_nnn'GTG Cuts at: 2245 3291 2245 Size: 1046 5345 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5398 809 Size: 507 4082 1802 Fragments arranged by size: 4082 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3571 3838 4727 Size: 54 1425 50 275 18 1741 267 889 Cuts at: 4727 6356 8 Size: 1629 43 Fragments arranged by size: 1741 1629 1425 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3838 1537 Size: 275 2026 4090 Fragments arranged by size: 4090 2026 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2092 2954 4321 1353 Size: 659 80 862 1367 3423 Fragments arranged by size: 3423 1367 862 659 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2509 3084 3320 4978 5352 5498 Size: 1064 138 920 575 236 1658 374 146 Cuts at: 5498 387 Size: 1280 Fragments arranged by size: 1658 1280 1064 920 575 374 236 146 138 EcoICRI GAG'CTC Cuts at: 3340 3340 Size: 6391 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2867 3413 3504 4454 5051 5838 6106 Size: 393 1235 546 91 950 597 787 268 Cuts at: 6106 1239 Size: 1524 Fragments arranged by size: 1524 1235 950 787 597 546 393 268 91 EcoO109I rG'GnC_Cy Cuts at: 2302 3504 3548 3604 2302 Size: 1202 44 56 5089 Fragments arranged by size: 5089 1202 56 44 EcoRI G'AATT_C Cuts at: 2722 3194 3700 4078 2722 Size: 472 506 378 5035 Fragments arranged by size: 5035 506 472 378 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 2442 5538 1678 Size: 764 3096 2531 Fragments arranged by size: 3096 2531 764 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 2870 2902 2948 Size: 32 135 32 971 32 295 32 46 Cuts at: 2948 2980 3353 3385 5032 5064 5106 5138 1373 Size: 32 373 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 971 373 295 135 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3735 3981 669 Size: 988 281 1797 246 3079 Fragments arranged by size: 3079 1797 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KasI G'GCGC_C Cuts at: 2438 2438 Size: 6391 KpnI G_GTAC'C Cuts at: 3716 3716 Size: 6391 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5505 5689 1305 Size: 4200 184 2007 Fragments arranged by size: 4200 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4569 Fragments arranged by size: 4569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4349 4708 4867 518 Size: 648 3183 359 159 2042 Fragments arranged by size: 3183 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2429 2490 3238 3258 3316 3429 3600 3807 Size: 823 61 748 20 58 113 171 207 Cuts at: 3807 4484 5632 5877 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 748 677 245 207 171 113 61 58 20 NarI GG'CG_CC Cuts at: 2439 2439 Size: 6391 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4900 Fragments arranged by size: 4900 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3475 3685 3749 5294 1132 Size: 97 2246 210 64 1545 2229 Fragments arranged by size: 2246 2229 1545 210 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 2779 2832 1108 Size: 1671 53 4667 Fragments arranged by size: 4667 1671 53 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 6391 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PmlI CAC'GTG Cuts at: 2382 2418 2842 2382 Size: 36 424 5931 Fragments arranged by size: 5931 424 36 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PspOMI G'GGCC_C Cuts at: 3503 3503 Size: 6391 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2519 2551 2519 Size: 32 6359 PstI C_TGCA'G Cuts at: 830 2129 2543 2615 3237 3315 3743 830 Size: 1299 414 72 622 78 428 3478 Fragments arranged by size: 3478 1299 622 428 414 78 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 3238 3316 3600 3238 Size: 78 284 6029 Fragments arranged by size: 6029 284 78 SacI G_AGCT'C Cuts at: 3342 3342 Size: 6391 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SapI GCTCTTCn'nnn_ Cuts at: 2954 2954 Size: 6391 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 2873 4639 6351 2873 Size: 1766 1712 2913 Fragments arranged by size: 2913 1766 1712 SfcI C'TryA_G Cuts at: 826 1045 2125 2407 2485 2539 2611 3233 3311 Size: 219 1080 282 78 54 72 622 78 Cuts at: 3311 3739 4874 5555 5746 826 Size: 428 1135 681 191 1471 Fragments arranged by size: 1471 1135 1080 681 622 428 282 219 191 78 78 72 54 SfoI GGC'GCC Cuts at: 2440 2440 Size: 6391 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3633 3718 1872 Size: 1761 85 4545 Fragments arranged by size: 4545 1761 85 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 2207 4315 5 Size: 47 2155 2108 2081 Fragments arranged by size: 2155 2108 2081 47 StuI AGG'CCT Cuts at: 1517 3365 1517 Size: 1848 4543 StyI C'CwwG_G Cuts at: 513 1065 2004 2270 3645 3830 513 Size: 552 939 266 1375 185 3074 Fragments arranged by size: 3074 1375 939 552 266 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4418 4435 4588 4773 5218 325 Size: 1255 2838 17 153 185 445 1498 Fragments arranged by size: 2838 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 2595 2871 Size: 274 80 33 51 1443 330 288 276 Cuts at: 2871 2913 3639 4637 6349 96 Size: 42 726 998 1712 138 Fragments arranged by size: 1712 1443 998 726 330 288 276 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2344 2376 3319 3351 6351 6383 2344 Size: 32 943 32 3000 32 2352 Fragments arranged by size: 3000 2352 943 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3631 3716 1870 Size: 1761 85 4545 Fragments arranged by size: 4545 1761 85 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI