Restriction map for
pFN21AB5684
(Linear) (Six-base) MAPSORT of: KIBB5684.seq Check: 3194 from: 1 to: 1686
KIBB5684.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 295 901 1686
Size: 295 606 785
Fragments arranged by size:
785 606 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1408 1686
Size: 862 546 278
Fragments arranged by size:
862 546 278
AlwNI CAG_nnn'CTG
Cuts at: 0 244 300 641 774 1046 1424 1686
Size: 244 56 341 133 272 378 262
Fragments arranged by size:
378 341 272 262 244 133 56
ApaI G_GGCC'C
Cuts at: 0 1502 1686
Size: 1502 184
ApaLI G'TGCA_C
Cuts at: 0 441 1686
Size: 441 1245
ApoI r'AATT_y
Cuts at: 0 717 1156 1189 1686
Size: 717 439 33 497
Fragments arranged by size:
717 497 439 33
AvaI C'yCGr_G
Cuts at: 0 1626 1686
Size: 1626 60
BanI G'GyrC_C
Cuts at: 0 283 433 697 830 1686
Size: 283 150 264 133 856
Fragments arranged by size:
856 283 264 150 133
BanII G_rGCy'C
Cuts at: 0 23 944 1337 1502 1686
Size: 23 921 393 165 184
Fragments arranged by size:
921 393 184 165 23
BbeI G_GCGC'C
Cuts at: 0 437 1686
Size: 437 1249
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 1686
Size: 45 1641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 356 679 713 1686
Size: 322 34 323 34 973
Fragments arranged by size:
973 323 322 34 34
BglII A'GATC_T
Cuts at: 0 103 1686
Size: 103 1583
Bme1580I G_kGCm'C
Cuts at: 0 228 286 331 412 445 768 881 1260
Size: 228 58 45 81 33 323 113 379
Cuts at: 1260 1502 1686
Size: 242 184
Fragments arranged by size:
379 323 242 228 184 113 81 58
45 33
BmgBI CAC'GTC
Cuts at: 0 860 1686
Size: 860 826
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 784 1686
Size: 755 29 902
Fragments arranged by size:
902 755 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 1686
Size: 1499 187
BsaAI yAC'GTr
Cuts at: 0 377 413 837 1686
Size: 377 36 424 849
Fragments arranged by size:
849 424 377 36
BsaHI Gr'CG_yC
Cuts at: 0 434 1520 1686
Size: 434 1086 166
Fragments arranged by size:
1086 434 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 943 973 1686
Size: 943 30 713
Fragments arranged by size:
943 713 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 1159 1162 1686
Size: 110 1049 3 524
Fragments arranged by size:
1049 524 110 3
BseYI C'CCAG_C
Cuts at: 0 64 343 510 1595 1686
Size: 64 279 167 1085 91
Fragments arranged by size:
1085 279 167 91 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1410 1686
Size: 1410 276
BsiEI CG_ry'CG
Cuts at: 0 1684 1686
Size: 1684 2
BsiHKAI G_wGCw'C
Cuts at: 0 182 421 445 1337 1686
Size: 182 239 24 892 349
Fragments arranged by size:
892 349 239 182 24
Bsp1286I G_dGCh'C
Cuts at: 0 23 182 228 286 331 412 421 445
Size: 23 159 46 58 45 81 9 24
Cuts at: 445 768 881 944 1260 1337 1502 1686
Size: 323 113 63 316 77 165 184
Fragments arranged by size:
323 316 184 165 159 113 81 77
63 58 46 45 24 23 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 295 901 1313 1686
Size: 295 606 412 373
Fragments arranged by size:
606 412 373 295
BsrBI CCG'CTC
Cuts at: 0 1014 1383 1493 1686
Size: 1014 369 110 193
Fragments arranged by size:
1014 369 193 110
BsrDI GCAATG_nn'
Cuts at: 0 14 1217 1686
Size: 14 1203 469
Fragments arranged by size:
1203 469 14
BsrGI T'GTAC_A
Cuts at: 0 1634 1686
Size: 1634 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1286 1686
Size: 1286 400
BstEII G'GTnAC_C
Cuts at: 0 1644 1686
Size: 1644 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1686
Size: 510 1176
BstYI r'GATC_y
Cuts at: 0 103 1686
Size: 103 1583
BtgI C'CryG_G
Cuts at: 0 669 712 1686
Size: 669 43 974
Fragments arranged by size:
974 669 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 939 1686
Size: 939 747
BtsI GCAGTG_nn'
Cuts at: 0 1068 1686
Size: 1068 618
DraIII CAC_nnn'GTG
Cuts at: 0 240 1286 1686
Size: 240 1046 400
Fragments arranged by size:
1046 400 240
EaeI y'GGCC_r
Cuts at: 0 1566 1686
Size: 1566 120
EarI CTCTTCn'nnn_
Cuts at: 0 7 87 949 1686
Size: 7 80 862 737
Fragments arranged by size:
862 737 80 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 504 1079 1315 1686
Size: 504 575 236 371
Fragments arranged by size:
575 504 371 236
EcoICRI GAG'CTC
Cuts at: 0 1335 1686
Size: 1335 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1408 1499 1686
Size: 862 546 91 187
Fragments arranged by size:
862 546 187 91
EcoO109I rG'GnC_Cy
Cuts at: 0 297 1499 1543 1599 1686
Size: 297 1202 44 56 87
Fragments arranged by size:
1202 297 87 56 44
EcoRI G'AATT_C
Cuts at: 0 717 1189 1686
Size: 717 472 497
Fragments arranged by size:
717 497 472
HaeII r_GCGC'y
Cuts at: 0 437 1686
Size: 437 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 865 897 943 975 1348 1380
Size: 538 32 295 32 46 32 373 32
Cuts at: 1380 1686
Size: 306
Fragments arranged by size:
538 373 306 295 46 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 433 1686
Size: 433 1253
MspA1I CmG'CkG
Cuts at: 0 424 485 1233 1253 1311 1424 1595 1686
Size: 424 61 748 20 58 113 171 91
Fragments arranged by size:
748 424 171 113 91 61 58 20
NarI GG'CG_CC
Cuts at: 0 434 1686
Size: 434 1252
NspI r_CATG'y
Cuts at: 0 1470 1680 1686
Size: 1470 210 6
Fragments arranged by size:
1470 210 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 774 827 1686
Size: 774 53 859
Fragments arranged by size:
859 774 53
PfoI T'CCnGG_A
Cuts at: 0 1076 1686
Size: 1076 610
PmlI CAC'GTG
Cuts at: 0 377 413 837 1686
Size: 377 36 424 849
Fragments arranged by size:
849 424 377 36
PspOMI G'GGCC_C
Cuts at: 0 1498 1686
Size: 1498 188
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 514 546 1686
Size: 514 32 1140
Fragments arranged by size:
1140 514 32
PstI C_TGCA'G
Cuts at: 0 124 538 610 1232 1310 1686
Size: 124 414 72 622 78 376
Fragments arranged by size:
622 414 376 124 78 72
PvuII CAG'CTG
Cuts at: 0 1233 1311 1595 1686
Size: 1233 78 284 91
Fragments arranged by size:
1233 284 91 78
SacI G_AGCT'C
Cuts at: 0 1337 1686
Size: 1337 349
SapI GCTCTTCn'nnn_
Cuts at: 0 949 1686
Size: 949 737
ScaI AGT'ACT
Cuts at: 0 868 1686
Size: 868 818
SfcI C'TryA_G
Cuts at: 0 120 402 480 534 606 1228 1306 1686
Size: 120 282 78 54 72 622 78 380
Fragments arranged by size:
622 380 282 120 78 78 72 54
SfoI GGC'GCC
Cuts at: 0 435 1686
Size: 435 1251
SmaI CCC'GGG
Cuts at: 0 1628 1686
Size: 1628 58
SspI AAT'ATT
Cuts at: 0 202 1686
Size: 202 1484
StuI AGG'CCT
Cuts at: 0 1360 1686
Size: 1360 326
StyI C'CwwG_G
Cuts at: 0 265 1640 1686
Size: 265 1375 46
Fragments arranged by size:
1375 265 46
TatI w'GTAC_w
Cuts at: 0 302 590 866 908 1634 1686
Size: 302 288 276 42 726 52
Fragments arranged by size:
726 302 288 276 52 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 339 371 1314 1346 1686
Size: 339 32 943 32 340
Fragments arranged by size:
943 340 339 32 32
XmaI C'CCGG_G
Cuts at: 0 1626 1686
Size: 1626 60
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglII Bme1580I BmgBI BmrI
BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI
MspA1I NarI NspI PflMI PfoI PmlI PspOMI PsrI
PstI PvuII SacI SapI ScaI SfcI SfoI SmaI
SspI StuI StyI TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglI BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SwaI TaqII
Tth111I XbaI XcmI XhoI XmnI ZraI