(Circular) (Six-base) MAPSORT of: pFN21AE5606.seq Check: 2953 from: 1 to: 6238 pFN21AE5606 6238 bp With 182 enzymes: SgfI * February 27, 2017 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3519 4301 5685 5953 1632 Size: 1887 782 1384 268 1917 Fragments arranged by size: 1917 1887 1384 782 268 AfeI AGC'GCT Cuts at: 2925 2925 Size: 6238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 2652 5137 6114 1203 Size: 1449 2485 977 1327 Fragments arranged by size: 2485 1449 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 2391 2709 3981 5553 2391 Size: 318 1272 1572 3076 Fragments arranged by size: 3076 1572 1272 318 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2282 2813 3447 3547 3773 3925 6034 1493 Size: 789 531 634 100 226 152 2109 1697 Fragments arranged by size: 2109 1697 789 634 531 226 152 100 AseI AT'TA_AT Cuts at: 160 1032 2346 3165 3423 4792 160 Size: 872 1314 819 258 1369 1606 Fragments arranged by size: 1606 1369 1314 872 819 258 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3563 1870 Size: 73 1620 4545 Fragments arranged by size: 4545 1620 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3213 3559 5014 618 Size: 325 217 627 1426 346 1455 1842 Fragments arranged by size: 1842 1455 1426 627 346 325 217 BanII G_rGCy'C Cuts at: 1391 2050 3557 1391 Size: 659 1507 4072 Fragments arranged by size: 4072 1507 659 BbeI G_GCGC'C Cuts at: 3217 3217 Size: 6238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2534 3371 928 Size: 971 635 837 3795 Fragments arranged by size: 3795 971 837 635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2264 6233 2264 Size: 3969 2269 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2896 3202 3969 Size: 416 53 83 399 24 1479 306 767 Cuts at: 3969 4927 442 Size: 958 1753 Fragments arranged by size: 1753 1479 958 767 416 399 306 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2682 2991 3432 4898 1239 Size: 1340 103 309 441 1466 2579 Fragments arranged by size: 2579 1466 1340 441 309 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2179 4901 882 Size: 617 680 2722 2219 Fragments arranged by size: 2722 2219 680 617 BsaAI yAC'GTr Cuts at: 493 1204 2572 4029 493 Size: 711 1368 1457 2702 Fragments arranged by size: 2702 1457 1368 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3214 4045 4427 5062 Size: 53 83 186 936 1681 831 382 635 Cuts at: 5062 275 Size: 1451 Fragments arranged by size: 1681 1451 936 831 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6013 Fragments arranged by size: 6013 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2988 1186 Size: 1802 4436 BseYI C'CCAG_C Cuts at: 2043 5441 5937 2043 Size: 3398 496 2344 Fragments arranged by size: 3398 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2394 1163 Size: 573 658 5007 Fragments arranged by size: 5007 658 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4299 4384 5455 4299 Size: 85 1071 5082 Fragments arranged by size: 5082 1071 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 3557 4299 4384 5455 1391 Size: 399 260 1507 742 85 1071 2174 Fragments arranged by size: 2174 1507 1071 742 399 260 85 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3593 844 Size: 2749 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3193 3250 4733 4907 65 Size: 1183 1945 57 1483 174 1396 Fragments arranged by size: 1945 1483 1396 1183 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 4882 5069 1700 Size: 110 3072 187 2869 Fragments arranged by size: 3072 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3009 96 Size: 2913 3325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 3057 4298 5310 3057 Size: 1241 1012 3985 Fragments arranged by size: 3985 1241 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3521 794 Size: 2727 3511 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2264 3502 3568 3935 4320 4337 5789 5875 Size: 292 1238 66 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1238 385 367 346 292 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5686 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 4603 Fragments arranged by size: 4603 896 683 56 BtsI GCAGTG_nn' Cuts at: 2788 3754 4548 4568 2788 Size: 966 794 20 4458 Fragments arranged by size: 4458 966 794 20 ClaI AT'CG_AT Cuts at: 1554 3208 1554 Size: 1654 4584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2800 3080 3256 Size: 54 1425 50 275 18 970 280 176 Cuts at: 3256 3685 4574 6203 8 Size: 429 889 1629 43 Fragments arranged by size: 1629 1425 970 889 429 280 275 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4168 1353 Size: 2815 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 2682 2991 3432 3519 4301 4898 Size: 393 947 103 309 441 87 782 597 Cuts at: 4898 5685 5953 1239 Size: 787 268 1524 Fragments arranged by size: 1524 947 787 782 597 441 393 309 268 103 87 EcoRI G'AATT_C Cuts at: 2813 3547 3925 2813 Size: 734 378 5126 Fragments arranged by size: 5126 734 378 EcoRV GAT'ATC Cuts at: 2491 2491 Size: 6238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2748 2748 Size: 6238 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2927 3217 5385 1678 Size: 1249 290 2168 2531 Fragments arranged by size: 2531 2168 1249 290 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2855 2887 2913 2945 3335 Size: 32 135 32 1283 32 26 32 390 Cuts at: 3335 3367 4879 4911 4953 4985 1373 Size: 32 1512 32 42 32 2626 Fragments arranged by size: 2626 1512 1283 390 135 42 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KasI G'GCGC_C Cuts at: 3213 3213 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3430 3837 3430 Size: 407 5831 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3004 5352 5536 1305 Size: 1699 2348 184 2007 Fragments arranged by size: 2348 2007 1699 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3082 3258 10 Size: 54 1425 343 1250 176 2990 Fragments arranged by size: 2990 1425 1250 343 176 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 4196 4555 4714 518 Size: 648 1189 1841 359 159 2042 Fragments arranged by size: 2042 1841 1189 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3654 4331 5479 5724 1606 Size: 2048 677 1148 245 2120 Fragments arranged by size: 2120 2048 1148 677 245 NarI GG'CG_CC Cuts at: 3214 3214 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5686 NdeI CA'TA_TG Cuts at: 387 2352 387 Size: 1965 4273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 2656 3596 5141 1132 Size: 97 1427 940 1545 2229 Fragments arranged by size: 2229 1545 1427 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 2652 5137 2652 Size: 2485 3753 PflMI CCAn_nnn'nTGG Cuts at: 1108 2444 1108 Size: 1336 4902 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2371 2403 2371 Size: 32 6206 PstI C_TGCA'G Cuts at: 830 2722 3590 830 Size: 1892 868 3478 Fragments arranged by size: 3478 1892 868 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SbfI CC_TGCA'GG Cuts at: 2722 3590 2722 Size: 868 5370 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 2718 3586 4721 5402 5593 826 Size: 219 1673 868 1135 681 191 1471 Fragments arranged by size: 1673 1471 1135 868 681 219 191 SfoI GGC'GCC Cuts at: 3215 3215 Size: 6238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4342 5243 5505 820 Size: 197 926 2399 901 262 1553 Fragments arranged by size: 2399 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6238 StyI C'CwwG_G Cuts at: 513 1065 2219 3677 513 Size: 552 1154 1458 3074 Fragments arranged by size: 3074 1458 1154 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2750 4265 4282 4435 4620 5065 325 Size: 1255 1170 1515 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1255 1170 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 4484 6196 Size: 274 80 33 51 1443 1032 1475 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1712 1475 1443 1032 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 3194 1316 Size: 1878 4360 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2445 3302 2445 Size: 857 5381 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI