Restriction map for
pFN21AE1532
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboII MfeI MflI MluI MroI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AciI 271 GCGG CCGC(-3/-1)
AflIII 101 ACATGT A^C[GA][CT]GT
BalI 261 TGGCCA TGG^CCA
BanII 92 GGGCTC G[GA]GC[CT]^C
BcgI 288 CGATCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 286 GCCGATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 92 GGGCTC G[AGT]GC[ACT]^C
Bsp1286I 92 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 101 ACATGT A^CATGT
BspMI 342 ACCTGC ACCTGC(4/8)
BsrI 282 ACTGG ACTGG(1/-1)
DraII 195 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 264 CCATGG C^C[GA][CT]GG
EcoNI 72 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 195 GGGACCT [GA]G^G[ACGT]CC[CT]
MluNI 261 TGGCCA TGG^CCA
MscI 261 TGGCCA TGG^CCA
NcoI 264 CCATGG C^CATGG
NlaIV 195 GGGACC GG[ACGT]^[ACGT]CC
NspI 101 ACATGT [GA]CATG^[CT]
PleI 277 GACTC GAGTC(4/5)
PpuMI 195 GGGACCT [GA]G^G[AT]CC[CT]
PstI 215 CTGCAG CTGCA^G
SfcI 215 CTGCAG C^T[GA][CT]AG
TfiI 254 GATTC G^A[AT]TC
Tsp509I 136 AATT ^AATT
TspRI 281 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 249 CCAGAGATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 5 AGCT AG^CT
58 AGCT
AvaI 95 CTCGGG C^[CT]CG[GA]G
167 CTCGGG
AvaII 36 GGTCC G^G[AT]CC
196 GGACC
BsmFI 98 GGGAC GGGAC(10/14)
195 GGGAC
BsoBI 95 CTCGGG C^[CT]CG[GA]G
167 CTCGGG
Cac8I 346 GCTGGC GC[ACGT]^[ACGT]GC
350 GCCTGC
DdeI 147 CTCAG C^T[ACGT]AG
356 CTGAG
DpnII 225 GATC ^GATC
289 GATC
EaeI 261 TGGCCA [CT]^GGCC[GA]
284 TGGCCG
HindIII 4 AAGCTT A^AGCTT
57 AAGCTT
HinfI 254 GATTC G^A[ACGT]TC
277 GACTC
MboI 225 GATC ^GATC
289 GATC
MnlI 79 GAGG CCTC(7/6)
274 GAGG
NdeII 225 GATC ^GATC
289 GATC
Sau3AI 225 GATC ^GATC
289 GATC
SfaNI 181 GATGC GCATC(5/9)
232 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
BstNI Cfr13I EcoRII EcoT14I Fnu4HI
HaeIII ItaI MvaI MwoI NlaIII
Sau96I ScrFI StyI TseI