(Linear) (Six-base) MAPSORT of: KIEE1532.seq  Check: 362  from: 1  to: 366



KIEE1532.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AflIII A'CryG_T

Cuts at:      0     101     366 
   Size:        101     265
 
AvaI C'yCGr_G

Cuts at:      0      95     167     366 
   Size:         95      72     199

  Fragments arranged by size: 

                 199      95      72
 
BanII G_rGCy'C

Cuts at:      0      96     366 
   Size:         96     270
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     277     311     366 
   Size:        277      34      55

  Fragments arranged by size: 

                 277      55      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     292     366 
   Size:        292      74
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     366 
   Size:         96     270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     351     366 
   Size:        351      15
 
BtgI C'CryG_G

Cuts at:      0     264     366 
   Size:        264     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     245     366 
   Size:        245     121
 
EaeI y'GGCC_r

Cuts at:      0     261     284     366 
   Size:        261      23      82

  Fragments arranged by size: 

                 261      82      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      76     366 
   Size:         76     290
 
EcoO109I rG'GnC_Cy

Cuts at:      0     196     366 
   Size:        196     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262     366 
   Size:        230      32     104

  Fragments arranged by size: 

                 230     104      32
 
HindIII A'AGCT_T

Cuts at:      0       4      57     366 
   Size:          4      53     309

  Fragments arranged by size: 

                 309      53       4
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     366 
   Size:        363       3
 
MscI TGG'CCA

Cuts at:      0     263     366 
   Size:        263     103
 
NcoI C'CATG_G

Cuts at:      0     264     366 
   Size:        264     102
 
NspI r_CATG'y

Cuts at:      0     105     366 
   Size:        105     261
 
PciI A'CATG_T

Cuts at:      0     101     366 
   Size:        101     265
 
PpuMI rG'GwC_Cy

Cuts at:      0     196     366 
   Size:        196     170
 
PstI C_TGCA'G

Cuts at:      0     219     366 
   Size:        219     147
 
SfcI C'TryA_G

Cuts at:      0     215     366 
   Size:        215     151
 
StyI C'CwwG_G

Cuts at:      0      32      72     264     366 
   Size:         32      40     192     102

  Fragments arranged by size: 

                 192     102      40      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      31     366 
   Size:         31     335
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     256     366 
   Size:        256     110
 

 Enzymes that do cut:

   AflIII     AvaI    BanII     BcgI     BglI Bsp1286I    BspMI     BtgI
    BtgZI     EaeI    EcoNI EcoO109I    Hin4I  HindIII     MmeI     MscI
     NcoI     NspI     PciI    PpuMI     PstI     SfcI     StyI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI