################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeII MamI MboII MfeI MflI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TseI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 318 GCGG CCGC(-3/-1) AcsI 45 GAATTT [GA]^AATT[CT] ApoI 45 GAATTT [GA]^AATT[CT] BalI 98 TGGCCA TGG^CCA BanI 19 GGCACC G^G[CT][GA]CC BcgI 41 GCAAGAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 31 CTAG C^TAG BmyI 18 GGGCAC G[AGT]GC[ACT]^C BsaI 304 GAGACC GGTCTC(1/5) Bsp1286I 18 GGGCAC G[AGT]GC[ACT]^C BsrI 258 ACTGG ACTGG(1/-1) BstXI 89 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 100 GCCAGC GC[ACGT]^[ACGT]GC DpnII 186 GATC ^GATC DraIII 68 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 232 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 357 CCACGG C^C[GA][CT]GG EaeI 98 TGGCCA [CT]^GGCC[GA] FauI 318 GCGGG CCCGC(4/6) HaeIII 99 GGCC GG^CC HgaI 7 GACGC GACGC(5/10) MaeI 31 CTAG C^TAG MboI 186 GATC ^GATC MluNI 98 TGGCCA TGG^CCA MscI 98 TGGCCA TGG^CCA MseI 194 TTAA T^TAA MslI 309 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 186 GATC ^GATC NlaIV 19 GGCACC GG[ACGT]^[ACGT]CC Sau3AI 186 GATC ^GATC Tru9I 194 TTAA T^TAA XcmI 248 CCATTGTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsaJI 15 CCTGGG C^C[ACGT][ACGT]GG 357 CCACGG BsmAI 217 GAGAC GTCTC(1/5) 304 GAGAC BsmFI 140 GGGAC GGGAC(10/14) 293 GGGAC BspMI 281 ACCTGC ACCTGC(4/8) 93 GCAGGT BstF5I 342 CATCC GGATG(2/0) 261 GGATG FokI 342 CATCC GGATG(9/13) 261 GGATG MaeIII 66 GTCAC ^GT[ACGT]AC 253 GTGAC MnlI 266 GAGG CCTC(7/6) 394 GAGG NlaIII 60 CATG CATG^ 105 CATG PstI 283 CTGCAG CTGCA^G 365 CTGCAG SfcI 283 CTGCAG C^T[GA][CT]AG 365 CTGCAG Tsp45I 66 GTCAC ^GT[GC]AC 253 GTGAC Tsp509I 46 AATT ^AATT 327 AATT TspRI 257 CACTG CA[GC]TG[ACGT][ACGT]^ 363 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI BssKI BstNI EcoRII HphI MvaI ScrFI TaqI TthHB8I