(Linear) (Six-base) MAPSORT of: KIEE2386.seq  Check: 8185  from: 1  to: 399



KIEE2386.orf

 With 182 enzymes: SgfI * 

                        November 15, 2011 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      84     290     399 
   Size:         84     206     109

  Fragments arranged by size: 

                 206     109      84
 
AleI CACnn'nnGTG

Cuts at:      0     313     399 
   Size:        313      86
 
ApoI r'AATT_y

Cuts at:      0      45     399 
   Size:         45     354
 
BanI G'GyrC_C

Cuts at:      0      19     399 
   Size:         19     380
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      30      64     399 
   Size:         30      34     335

  Fragments arranged by size: 

                 335      34      30
 
Bme1580I G_kGCm'C

Cuts at:      0      22     399 
   Size:         22     377
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     399 
   Size:        298     101
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     399 
   Size:         22     377
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      84     290     399 
   Size:         84     206     109

  Fragments arranged by size: 

                 206     109      84
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96     399 
   Size:         96     303
 
BtgI C'CryG_G

Cuts at:      0     357     399 
   Size:        357      42
 
BtsI GCAGTG_nn'

Cuts at:      0     362     399 
   Size:        362      37
 
DraIII CAC_nnn'GTG

Cuts at:      0      73     399 
   Size:         73     326
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     238     399 
   Size:        238     161
 
EaeI y'GGCC_r

Cuts at:      0      98     399 
   Size:         98     301
 
MscI TGG'CCA

Cuts at:      0     100     399 
   Size:        100     299
 
MslI CAynn'nnrTG

Cuts at:      0     313     399 
   Size:        313      86
 
PstI C_TGCA'G

Cuts at:      0     287     369     399 
   Size:        287      82      30

  Fragments arranged by size: 

                 287      82      30
 
SfcI C'TryA_G

Cuts at:      0     283     365     399 
   Size:        283      82      34

  Fragments arranged by size: 

                 283      82      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     370     399 
   Size:        370      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     399 
   Size:        255     144
 

 Enzymes that do cut:

     AarI     AleI     ApoI     BanI     BcgI Bme1580I     BsaI Bsp1286I
    BspMI    BstXI     BtgI     BtsI   DraIII     DrdI     EaeI     MscI
     MslI     PstI     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI