(Circular) (Six-base) MAPSORT of: pFN21AB5937.seq Check: 2529 from: 1 to: 6229 pFN21AB5937 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2299 2523 2534 3302 4292 5676 5944 Size: 488 179 224 11 768 990 1384 268 Cuts at: 5944 1632 Size: 1917 Fragments arranged by size: 1917 1384 990 768 488 268 224 179 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 3357 5128 6105 1203 Size: 2154 1771 977 1327 Fragments arranged by size: 2154 1771 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2714 2746 2019 Size: 32 663 32 5502 Fragments arranged by size: 5502 663 32 32 AlwNI CAG_nnn'CTG Cuts at: 2143 3972 5544 2143 Size: 1829 1572 2828 Fragments arranged by size: 2828 1829 1572 ApaI G_GGCC'C Cuts at: 2373 2373 Size: 6229 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2217 2373 3307 3350 3554 1870 Size: 73 274 156 934 43 204 4545 Fragments arranged by size: 4545 934 274 204 156 73 43 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 3550 5005 618 Size: 325 217 627 333 1430 1455 1842 Fragments arranged by size: 1842 1455 1430 627 333 325 217 BanII G_rGCy'C Cuts at: 1391 2373 2585 2768 3249 3548 1391 Size: 982 212 183 481 299 4072 Fragments arranged by size: 4072 982 481 299 212 183 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 6229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 3307 928 Size: 971 623 785 3850 Fragments arranged by size: 3850 971 785 623 BbvCI CC'TCA_GC Cuts at: 2480 2480 Size: 6229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2195 2229 4420 4454 1903 Size: 34 258 34 2191 34 3678 Fragments arranged by size: 3678 2191 258 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 3402 1548 Size: 1854 4375 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 3343 4840 Size: 107 122 71 631 605 409 1262 1497 Cuts at: 4840 136 Size: 1525 Fragments arranged by size: 1525 1497 1262 631 605 409 122 107 71 BglII A'GATC_T Cuts at: 2712 6224 2712 Size: 3512 2717 BlpI GC'TnA_GC Cuts at: 2088 2579 3646 2088 Size: 491 1067 4671 Fragments arranged by size: 4671 1067 491 Bme1580I G_kGCm'C Cuts at: 1790 2373 4290 5446 1790 Size: 583 1917 1156 2573 Fragments arranged by size: 2573 1917 1156 583 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2292 2533 Size: 416 53 83 399 24 777 98 241 Cuts at: 2533 2695 3960 4918 442 Size: 162 1265 958 1753 Fragments arranged by size: 1753 1265 958 777 416 399 241 162 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2611 3525 2611 Size: 914 5315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2408 2581 2741 3407 4889 1239 Size: 827 342 173 160 666 1482 2579 Fragments arranged by size: 2579 1482 827 666 342 173 160 Bpu10I CC'TnA_GC Cuts at: 2480 2480 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 4354 5219 5517 2672 Size: 1682 865 298 3384 Fragments arranged by size: 3384 1682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3077 3152 4892 882 Size: 617 1578 75 1740 2219 Fragments arranged by size: 2219 1740 1578 617 75 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 2121 2784 3093 Size: 53 83 186 936 518 70 663 309 Cuts at: 3093 4036 4418 5053 275 Size: 943 382 635 1451 Fragments arranged by size: 1451 943 936 663 635 518 382 309 186 83 70 53 BsaWI w'CCGG_w Cuts at: 3043 3075 4662 5060 5334 5481 5892 3043 Size: 32 1587 398 274 147 411 3380 Fragments arranged by size: 3380 1587 411 398 274 147 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 2714 2744 1178 Size: 30 165 30 616 30 665 30 4663 Fragments arranged by size: 4663 665 616 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2485 1186 Size: 1036 263 4930 Fragments arranged by size: 4930 1036 263 BseYI C'CCAG_C Cuts at: 2092 2143 2455 2799 2809 3196 3248 5432 5928 Size: 51 312 344 10 387 52 2184 496 Cuts at: 5928 2092 Size: 2393 Fragments arranged by size: 2393 2184 496 387 344 312 52 51 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 1163 Size: 573 696 4960 Fragments arranged by size: 4960 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3067 3679 4440 5468 1540 Size: 275 186 1066 612 761 1028 2301 Fragments arranged by size: 2301 1066 1028 761 612 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2768 4290 4375 5446 2106 Size: 662 1522 85 1071 2889 Fragments arranged by size: 2889 1522 1071 662 85 BsmI GAATG_Cn' Cuts at: 2253 3740 3833 2253 Size: 1487 93 4649 Fragments arranged by size: 4649 1487 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2373 2585 2768 3249 3548 4290 Size: 399 316 267 212 183 481 299 742 Cuts at: 4290 4375 5446 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 481 399 316 299 267 212 183 85 BspEI T'CCGG_A Cuts at: 3075 5892 3075 Size: 2817 3412 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3462 3584 844 Size: 2618 122 3489 Fragments arranged by size: 3489 2618 122 BsrBI CCG'CTC Cuts at: 1394 1823 2697 4116 1394 Size: 429 874 1419 3507 Fragments arranged by size: 3507 1419 874 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3366 4724 4898 65 Size: 1183 2118 1358 174 1396 Fragments arranged by size: 2118 1396 1358 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2616 4873 5060 1700 Size: 110 806 2257 187 2869 Fragments arranged by size: 2869 2257 806 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 2248 5033 2248 Size: 2785 3444 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3103 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2712 2845 3559 3926 4311 4328 5780 5866 Size: 740 133 714 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 740 714 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2647 3339 513 Size: 552 939 643 692 3403 Fragments arranged by size: 3403 939 692 643 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 2545 3745 4539 4559 2545 Size: 1200 794 20 4215 Fragments arranged by size: 4215 1200 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DraIII CAC_nnn'GTG Cuts at: 3448 3448 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2668 3676 4565 Size: 54 1425 50 275 18 838 1008 889 Cuts at: 4565 6194 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1008 889 838 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 2480 2795 4159 1353 Size: 770 357 315 1364 3423 Fragments arranged by size: 3423 1364 770 357 315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2039 3152 4816 5190 5336 Size: 1064 138 435 15 1113 1664 374 146 Cuts at: 5336 387 Size: 1280 Fragments arranged by size: 1664 1280 1113 1064 435 374 146 138 15 EcoICRI GAG'CTC Cuts at: 2766 2766 Size: 6229 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2120 2299 2408 2523 2534 2581 Size: 393 434 54 179 109 115 11 47 Cuts at: 2581 2741 3302 3407 4292 4889 5676 5944 1239 Size: 160 561 105 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 597 561 434 393 268 179 160 115 109 105 54 47 11 EcoO109I rG'GnC_Cy Cuts at: 2266 2370 2857 3376 3504 2266 Size: 104 487 519 128 4991 Fragments arranged by size: 4991 519 487 128 104 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3052 3052 Size: 6229 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2076 2124 2144 3003 3394 5376 1678 Size: 398 48 20 859 391 1982 2531 Fragments arranged by size: 2531 1982 859 398 391 48 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2890 2922 3344 3376 4870 Size: 32 135 32 1318 32 422 32 1494 Cuts at: 4870 4902 4944 4976 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1494 1318 422 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2120 2120 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 2731 3280 5343 5527 1305 Size: 1050 376 549 2063 184 2007 Fragments arranged by size: 2063 2007 1050 549 376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 3257 4187 4546 4705 518 Size: 648 1572 519 930 359 159 2042 Fragments arranged by size: 2042 1572 930 648 519 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 2428 2667 2683 2852 2887 3645 4322 Size: 810 12 239 16 169 35 758 677 Cuts at: 4322 5470 5715 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 810 758 677 245 239 169 35 16 12 NarI GG'CG_CC Cuts at: 2121 2121 Size: 6229 NcoI C'CATG_G Cuts at: 513 1065 2004 3339 513 Size: 552 939 1335 3403 Fragments arranged by size: 3403 1335 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 2184 2337 3166 1108 Size: 1005 71 153 829 4171 Fragments arranged by size: 4171 1005 829 153 71 PfoI T'CCnGG_A Cuts at: 2718 3426 2718 Size: 708 5521 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2019 2051 2714 2746 4298 4330 5811 5843 2019 Size: 32 663 32 1552 32 1481 32 2405 Fragments arranged by size: 2405 1552 1481 663 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2369 2369 Size: 6229 PstI C_TGCA'G Cuts at: 830 2865 3459 3581 830 Size: 2035 594 122 3478 Fragments arranged by size: 3478 2035 594 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2428 2428 Size: 6229 SacI G_AGCT'C Cuts at: 2768 2768 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SapI GCTCTTCn'nnn_ Cuts at: 2123 2123 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 3053 4477 6189 3053 Size: 1424 1712 3093 Fragments arranged by size: 3093 1712 1424 SfcI C'TryA_G Cuts at: 826 1045 2861 3455 3577 4712 5393 5584 826 Size: 219 1816 594 122 1135 681 191 1471 Fragments arranged by size: 1816 1471 1135 681 594 219 191 122 SfoI GGC'GCC Cuts at: 2122 2122 Size: 6229 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2219 2375 3556 1872 Size: 347 156 1181 4545 Fragments arranged by size: 4545 1181 347 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 4333 5234 5496 820 Size: 197 926 744 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 744 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 3279 1517 Size: 1762 4467 StyI C'CwwG_G Cuts at: 513 1065 2004 3153 3274 3339 3668 513 Size: 552 939 1149 121 65 329 3074 Fragments arranged by size: 3074 1149 939 552 329 121 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2160 2515 2823 3280 3352 4256 4273 Size: 1255 580 355 308 457 72 904 17 Cuts at: 4273 4426 4611 5056 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 904 580 457 445 355 308 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3051 4475 6187 Size: 274 80 33 51 1443 1074 1424 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1443 1424 1074 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2879 1316 Size: 1563 4666 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2305 2305 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2217 2373 3554 1870 Size: 347 156 1181 4545 Fragments arranged by size: 4545 1181 347 156 XmnI GAAnn'nnTTC Cuts at: 3035 4358 3035 Size: 1323 4906 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI