################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MfeI MflI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 132 CGCG CG^CG AccIII 756 TCCGGA T^CCGGA AlwI 836 GGATC GGATC(4/5) AocI 625 CCTGAGG CC^T[ACGT]AGG Asp700I 79 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 179 GAAGAC GAAGAC(2/6) BbvCI 316 GCTGAGG CCTCAGC(-5/-2) BfaI 454 CTAG C^TAG BglI 307 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 614 GCTCAGC GC^T[ACGT]AGC Bpu1102I 614 GCTCAGC GC^T[ACGT]AGC BpuAI 179 GAAGAC GAAGAC(2/6) BsaI 722 GGTCTC GGTCTC(1/5) BsaWI 756 TCCGGA [AT]^CCGG[AT] BseAI 756 TCCGGA T^CCGGA BspEI 756 TCCGGA T^CCGGA BspMI 610 ACCTGC ACCTGC(4/8) BsrFI 765 ACCGGC [GA]^CCGG[CT] BstAPI 9 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 135 GGTGACC G^GT[ACGT]ACC BstPI 135 GGTGACC G^GT[ACGT]ACC BstUI 132 CGCG CG^CG Bsu36I 625 CCTGAGG CC^T[ACGT]AGG CelII 614 GCTCAGC GC^T[ACGT]AGC Cfr10I 765 ACCGGC [GA]^CCGG[CT] DpnII 837 GATC ^GATC DrdI 154 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 625 CCTGAGG CC^T[ACGT]AGG EcoO65I 135 GGTGACC G^GT[ACGT]ACC FauI 130 CCCGC CCCGC(4/6) HgaI 692 GACGC GACGC(5/10) HinfI 161 GAGTC G^A[ACGT]TC KspI 131 CCGCGG CCGC^GG MaeI 454 CTAG C^TAG MboI 837 GATC ^GATC MroI 756 TCCGGA T^CCGGA MvnI 132 CGCG CG^CG NdeII 837 GATC ^GATC PleI 161 GAGTC GAGTC(4/5) PstI 745 CTGCAG CTGCA^G SacII 131 CCGCGG CCGC^GG SapI 18 GCTCTTC GCTCTTC(1/4) Sau3AI 837 GATC ^GATC SfcI 745 CTGCAG C^T[GA][CT]AG SmaI 74 CCCGGG CCC^GGG XmaCI 74 CCCGGG C^CCGGG XmaI 74 CCCGGG C^CCGGG XmnI 79 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 608 GTAC GT^AC 843 GTAC AflIII 338 ACATGT A^C[GA][CT]GT 1014 ACATGT AvaI 74 CCCGGG C^[CT]CG[GA]G 220 CCCGAG BalI 89 TGGCCA TGG^CCA 542 TGGCCA BpmI 64 CTGGAG CTGGAG(16/14) 158 CTGGAG BseRI 229 GAGGAG GAGGAG(10/8) 319 GAGGAG BsoBI 74 CCCGGG C^[CT]CG[GA]G 220 CCCGAG BspLU11I 338 ACATGT A^CATGT 1014 ACATGT BstXI 296 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 497 CCATGTCTTTGG CfoI 594 GCGC GCG^C 738 GCGC DraIII 38 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 668 CACCCAGTG DsaI 131 CCGCGG C^C[GA][CT]GG 485 CCGTGG HhaI 594 GCGC GCG^C 738 GCGC HinP1I 594 GCGC G^CGC 738 GCGC MluNI 89 TGGCCA TGG^CCA 542 TGGCCA MscI 89 TGGCCA TGG^CCA 542 TGGCCA PpuMI 152 GGGACCC [GA]G^G[AT]CC[CT] 480 AGGACCC RsaI 608 GTAC GT^AC 843 GTAC SfaNI 115 GATGC GCATC(5/9) 268 GATGC StuI 456 AGGCCT AGG^CCT 751 AGGCCT XcmI 632 CCACGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 864 CCACTACTGTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwNI ApaI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PflMI PvuII Sau96I ScrFI StyI TseI Tsp45I TspRI Van91I