(Circular) (Six-base) MAPSORT of: pFN21AE3652.seq Check: 604 from: 1 to: 5803 pFN21AE3652 5803 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2320 2370 3866 5250 5518 1632 Size: 688 50 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 688 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 2343 3019 4702 5679 1203 Size: 1140 676 1683 977 1327 Fragments arranged by size: 1683 1327 1140 977 676 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3108 1184 Size: 1924 3879 AlwNI CAG_nnn'CTG Cuts at: 2068 2824 2927 3546 5118 2068 Size: 756 103 619 1572 2753 Fragments arranged by size: 2753 1572 756 619 103 ApaI G_GGCC'C Cuts at: 2110 2133 2977 2110 Size: 23 844 4936 Fragments arranged by size: 4936 844 23 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2225 3128 1870 Size: 73 136 146 903 4545 Fragments arranged by size: 4545 903 146 136 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 4579 618 Size: 325 217 627 1337 1455 1842 Fragments arranged by size: 1842 1455 1337 627 325 217 BanII G_rGCy'C Cuts at: 1391 2110 2133 2219 2226 2454 2695 2935 2977 Size: 719 23 86 7 228 241 240 42 Cuts at: 2977 3122 1391 Size: 145 4072 Fragments arranged by size: 4072 719 241 240 228 145 86 42 23 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2191 928 Size: 971 292 4540 Fragments arranged by size: 4540 971 292 BbvCI CC'TCA_GC Cuts at: 2322 2322 Size: 5803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 4414 136 Size: 107 122 71 631 605 646 2096 1525 Fragments arranged by size: 2096 1525 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 2620 3220 2620 Size: 600 5203 Bme1580I G_kGCm'C Cuts at: 1790 2055 2110 2133 2429 2502 2977 3072 3864 Size: 265 55 23 296 73 475 95 792 Cuts at: 3864 5020 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 792 475 296 265 95 73 55 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 2670 2850 Size: 416 53 83 399 24 1122 131 180 Cuts at: 2850 3534 4492 442 Size: 684 958 1753 Fragments arranged by size: 1753 1122 958 684 416 399 180 131 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2184 4463 1239 Size: 851 94 2279 2579 Fragments arranged by size: 2579 2279 851 94 Bpu10I CC'TnA_GC Cuts at: 2322 2322 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2733 4466 882 Size: 617 1234 1733 2219 Fragments arranged by size: 2219 1733 1234 617 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3610 3992 4627 275 Size: 53 83 186 936 2077 382 635 1451 Fragments arranged by size: 2077 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2761 4236 4634 4908 5055 5466 2761 Size: 1475 398 274 147 411 3098 Fragments arranged by size: 3098 1475 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2475 2505 1178 Size: 30 165 30 1072 30 4476 Fragments arranged by size: 4476 1072 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2339 1186 Size: 1063 90 4650 Fragments arranged by size: 4650 1063 90 BseYI C'CCAG_C Cuts at: 2263 2358 2391 2824 2829 2944 2949 2976 5006 Size: 95 33 433 5 115 5 27 2030 Cuts at: 5006 5502 2263 Size: 496 2564 Fragments arranged by size: 2564 2030 496 433 115 95 33 27 5 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1997 2525 2879 1163 Size: 573 261 528 354 4087 Fragments arranged by size: 4087 573 528 354 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3864 3949 5020 3864 Size: 85 1071 4647 Fragments arranged by size: 4647 1071 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2110 2133 2219 2226 2429 2454 Size: 399 265 55 23 86 7 203 25 Cuts at: 2454 2502 2695 2935 2977 3072 3122 3864 3949 Size: 48 193 240 42 95 50 742 85 Cuts at: 3949 5020 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 265 240 203 193 95 86 85 55 50 48 42 25 23 7 BspEI T'CCGG_A Cuts at: 2761 5466 2761 Size: 2705 3098 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2624 3158 844 Size: 1780 534 3489 Fragments arranged by size: 3489 1780 534 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2770 4447 4634 1700 Size: 110 960 1677 187 2869 Fragments arranged by size: 2869 1677 960 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 794 Size: 1226 4577 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 2140 1184 Size: 956 4847 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2509 2308 Size: 201 5602 BstYI r'GATC_y Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798 Size: 1161 367 385 17 1452 86 12 346 Cuts at: 5798 1972 Size: 1977 Fragments arranged by size: 1977 1452 1161 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2631 2631 Size: 5803 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2490 513 Size: 552 939 132 354 3826 Fragments arranged by size: 3826 939 552 354 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 3319 4113 4133 3319 Size: 794 20 4989 Fragments arranged by size: 4989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DraIII CAC_nnn'GTG Cuts at: 2048 2678 2048 Size: 630 5173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2165 4810 809 Size: 507 849 2645 1802 Fragments arranged by size: 2645 1802 849 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2351 2547 Size: 54 1425 50 275 18 264 257 196 Cuts at: 2547 3250 4139 5768 8 Size: 703 889 1629 43 Fragments arranged by size: 1629 1425 889 703 275 264 257 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2226 2339 3733 1353 Size: 677 196 113 1394 3423 Fragments arranged by size: 3423 1394 677 196 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2184 2320 2370 3866 4463 5250 Size: 393 458 94 136 50 1496 597 787 Cuts at: 5250 5518 1239 Size: 268 1524 Fragments arranged by size: 1524 1496 787 597 458 393 268 136 94 50 EcoO109I rG'GnC_Cy Cuts at: 2106 2107 2129 2130 2158 2329 2486 2973 3012 Size: 1 22 1 28 171 157 487 39 Cuts at: 3012 2106 Size: 4897 Fragments arranged by size: 4897 487 171 157 39 28 22 1 1 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2273 2273 Size: 5803 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 4444 4476 4518 Size: 32 135 32 704 32 2136 32 42 Cuts at: 4518 4550 1373 Size: 32 2626 Fragments arranged by size: 2626 2136 704 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2549 10 Size: 54 1425 343 264 453 3264 Fragments arranged by size: 3264 1425 453 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3761 4120 4279 518 Size: 648 2595 359 159 2042 Fragments arranged by size: 2595 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2138 2280 2297 2395 3219 3896 5044 Size: 452 80 142 17 98 824 677 1148 Cuts at: 5044 5289 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 824 677 452 245 142 98 80 17 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4312 Fragments arranged by size: 4312 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2192 2347 3023 3161 4706 1132 Size: 97 963 155 676 138 1545 2229 Fragments arranged by size: 2229 1545 963 676 155 138 97 PasI CC'CwG_GG Cuts at: 1348 2194 2927 2999 1348 Size: 846 733 72 4152 Fragments arranged by size: 4152 846 733 72 PciI A'CATG_T Cuts at: 2343 3019 4702 2343 Size: 676 1683 3444 Fragments arranged by size: 3444 1683 676 PflMI CCAn_nnn'nTGG Cuts at: 1108 2048 2068 2678 2824 2927 1108 Size: 940 20 610 146 103 3984 Fragments arranged by size: 3984 940 610 146 103 20 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2486 2158 Size: 328 5475 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2106 2129 2973 2106 Size: 23 844 4936 Fragments arranged by size: 4936 844 23 PstI C_TGCA'G Cuts at: 830 2754 3155 830 Size: 1924 401 3478 Fragments arranged by size: 3478 1924 401 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2058 2280 2297 2395 2058 Size: 222 17 98 5466 Fragments arranged by size: 5466 222 98 17 SacII CC_GC'GG Cuts at: 2139 2139 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SanDI GG'GwC_CC Cuts at: 2158 2158 Size: 5803 SapI GCTCTTCn'nnn_ Cuts at: 2030 2030 Size: 5803 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2750 3151 4286 4967 5158 826 Size: 219 1705 401 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 681 401 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 2081 3130 1872 Size: 209 1049 4545 Fragments arranged by size: 4545 1049 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 2463 2758 1517 Size: 946 295 4562 Fragments arranged by size: 4562 946 295 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2124 2723 3242 513 Size: 552 939 93 27 599 519 3074 Fragments arranged by size: 3074 939 599 552 519 93 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2689 3830 3847 4000 4185 4630 325 Size: 1255 1109 1141 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1141 1109 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4049 5761 96 Size: 274 80 33 51 1443 2072 1712 138 Fragments arranged by size: 2072 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2475 2507 5763 5795 2475 Size: 32 3256 32 2483 Fragments arranged by size: 3256 2483 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2644 2876 2644 Size: 232 5571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 2079 3128 1870 Size: 209 1049 4545 Fragments arranged by size: 4545 1049 209 XmnI GAAnn'nnTTC Cuts at: 2088 3932 2088 Size: 1844 3959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI