Restriction map for
pFN21AE3652
(Linear) (Six-base) MAPSORT of: KIEE3652.seq Check: 4881 from: 1 to: 1098
KIEE3652.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 365 1098
Size: 315 50 733
Fragments arranged by size:
733 315 50
AflIII A'CryG_T
Cuts at: 0 338 1014 1098
Size: 338 676 84
Fragments arranged by size:
676 338 84
AlwNI CAG_nnn'CTG
Cuts at: 0 63 819 922 1098
Size: 63 756 103 176
Fragments arranged by size:
756 176 103 63
ApaI G_GGCC'C
Cuts at: 0 105 128 972 1098
Size: 105 23 844 126
Fragments arranged by size:
844 126 105 23
AvaI C'yCGr_G
Cuts at: 0 74 220 1098
Size: 74 146 878
Fragments arranged by size:
878 146 74
BanII G_rGCy'C
Cuts at: 0 105 128 214 221 449 690 930 972
Size: 105 23 86 7 228 241 240 42
Cuts at: 972 1098
Size: 126
Fragments arranged by size:
241 240 228 126 105 86 42 23
7
BbsI GAAGACnn'nnnn_
Cuts at: 0 186 1098
Size: 186 912
BbvCI CC'TCA_GC
Cuts at: 0 317 1098
Size: 317 781
BglI GCCn_nnn'nGGC
Cuts at: 0 313 1098
Size: 313 785
BlpI GC'TnA_GC
Cuts at: 0 615 1098
Size: 615 483
Bme1580I G_kGCm'C
Cuts at: 0 50 105 128 424 497 972 1067 1098
Size: 50 55 23 296 73 475 95 31
Fragments arranged by size:
475 296 95 73 55 50 31 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 665 845 1098
Size: 534 131 180 253
Fragments arranged by size:
534 253 180 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 1098
Size: 255 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 179 1098
Size: 85 94 919
Fragments arranged by size:
919 94 85
Bpu10I CC'TnA_GC
Cuts at: 0 317 1098
Size: 317 781
BsaI GGTCTCn'nnnn_
Cuts at: 0 728 1098
Size: 728 370
BsaWI w'CCGG_w
Cuts at: 0 756 1098
Size: 756 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 500 1098
Size: 470 30 598
Fragments arranged by size:
598 470 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 334 1098
Size: 244 90 764
Fragments arranged by size:
764 244 90
BseYI C'CCAG_C
Cuts at: 0 258 353 386 819 824 939 944 971
Size: 258 95 33 433 5 115 5 27
Cuts at: 971 1098
Size: 127
Fragments arranged by size:
433 258 127 115 95 33 27 5
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 874 1098
Size: 520 354 224
Fragments arranged by size:
520 354 224
Bsp1286I G_dGCh'C
Cuts at: 0 50 105 128 214 221 424 449 497
Size: 50 55 23 86 7 203 25 48
Cuts at: 497 690 930 972 1067 1098
Size: 193 240 42 95 31
Fragments arranged by size:
240 203 193 95 86 55 50 48
42 31 25 23 7
BspEI T'CCGG_A
Cuts at: 0 756 1098
Size: 756 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 619 1098
Size: 619 479
BsrFI r'CCGG_y
Cuts at: 0 765 1098
Size: 765 333
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 1098
Size: 15 1083
BstEII G'GTnAC_C
Cuts at: 0 135 1098
Size: 135 963
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 504 1098
Size: 303 201 594
Fragments arranged by size:
594 303 201
Bsu36I CC'TnA_GG
Cuts at: 0 626 1098
Size: 626 472
BtgI C'CryG_G
Cuts at: 0 131 485 1098
Size: 131 354 613
Fragments arranged by size:
613 354 131
DraIII CAC_nnn'GTG
Cuts at: 0 43 673 1098
Size: 43 630 425
Fragments arranged by size:
630 425 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 160 1098
Size: 160 938
EaeI y'GGCC_r
Cuts at: 0 89 346 542 1098
Size: 89 257 196 556
Fragments arranged by size:
556 257 196 89
EarI CTCTTCn'nnn_
Cuts at: 0 25 221 334 1098
Size: 25 196 113 764
Fragments arranged by size:
764 196 113 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 179 315 365 1098
Size: 85 94 136 50 733
Fragments arranged by size:
733 136 94 85 50
EcoO109I rG'GnC_Cy
Cuts at: 0 101 102 124 125 153 324 481 968
Size: 101 1 22 1 28 171 157 487
Cuts at: 968 1007 1098
Size: 39 91
Fragments arranged by size:
487 171 157 101 91 39 28 22
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 268 1098
Size: 268 830
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1098
Size: 271 32 795
Fragments arranged by size:
795 271 32
MscI TGG'CCA
Cuts at: 0 91 544 1098
Size: 91 453 554
Fragments arranged by size:
554 453 91
MspA1I CmG'CkG
Cuts at: 0 53 133 275 292 390 1098
Size: 53 80 142 17 98 708
Fragments arranged by size:
708 142 98 80 53 17
NspI r_CATG'y
Cuts at: 0 187 342 1018 1098
Size: 187 155 676 80
Fragments arranged by size:
676 187 155 80
PasI CC'CwG_GG
Cuts at: 0 189 922 994 1098
Size: 189 733 72 104
Fragments arranged by size:
733 189 104 72
PciI A'CATG_T
Cuts at: 0 338 1014 1098
Size: 338 676 84
Fragments arranged by size:
676 338 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 43 63 673 819 922 1098
Size: 43 20 610 146 103 176
Fragments arranged by size:
610 176 146 103 43 20
PfoI T'CCnGG_A
Cuts at: 0 812 1098
Size: 812 286
PpuMI rG'GwC_Cy
Cuts at: 0 153 481 1098
Size: 153 328 617
Fragments arranged by size:
617 328 153
PspOMI G'GGCC_C
Cuts at: 0 101 124 968 1098
Size: 101 23 844 130
Fragments arranged by size:
844 130 101 23
PstI C_TGCA'G
Cuts at: 0 749 1098
Size: 749 349
PvuII CAG'CTG
Cuts at: 0 53 275 292 390 1098
Size: 53 222 17 98 708
Fragments arranged by size:
708 222 98 53 17
SacII CC_GC'GG
Cuts at: 0 134 1098
Size: 134 964
SanDI GG'GwC_CC
Cuts at: 0 153 1098
Size: 153 945
SapI GCTCTTCn'nnn_
Cuts at: 0 25 1098
Size: 25 1073
SfcI C'TryA_G
Cuts at: 0 745 1098
Size: 745 353
SmaI CCC'GGG
Cuts at: 0 76 1098
Size: 76 1022
StuI AGG'CCT
Cuts at: 0 458 753 1098
Size: 458 295 345
Fragments arranged by size:
458 345 295
StyI C'CwwG_G
Cuts at: 0 92 119 718 1098
Size: 92 27 599 380
Fragments arranged by size:
599 380 92 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 684 1098
Size: 684 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 470 502 1098
Size: 470 32 596
Fragments arranged by size:
596 470 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 639 871 1098
Size: 639 232 227
Fragments arranged by size:
639 232 227
XmaI C'CCGG_G
Cuts at: 0 74 1098
Size: 74 1024
XmnI GAAnn'nnTTC
Cuts at: 0 83 1098
Size: 83 1015
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanII BbsI BbvCI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI
BsrFI BstAPI BstEII BstXI Bsu36I BtgI DraIII DrdI
EaeI EarI Eco57MI EcoO109I FalI Hin4I MscI MspA1I
NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII SacII SanDI SapI SfcI SmaI StuI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BpuEI BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI ZraI