################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AccI AclI AflII Alw44I AocI Aor51HI ApaLI AscI Asp700I AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsgI BsiEI BsmI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI MamI MluI MluNI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PmeI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI Sse8387I SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 378 GGTACC G^GTACC AccIII 181 TCCGGA T^CCGGA AcyI 1290 GACGCC G[GA]^CG[CT]C AgeI 375 ACCGGT A^CCGGT AhdI 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 830 GGATC GGATC(4/5) ApaI 315 GGGCCC GGGCC^C AseI 1059 ATTAAT AT^TAAT AsnI 1059 ATTAAT AT^TAAT Asp718I 378 GGTACC G^GTACC AspEI 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1302 GGACC G^G[AT]CC BbrPI 60 CACGTG CAC^GTG BbsI 654 GTCTTC GAAGAC(2/6) BpuAI 654 GTCTTC GAAGAC(2/6) BsaHI 1290 GACGCC G[GA]^CG[CT]C BsaI 1020 GAGACC GGTCTC(1/5) BseAI 181 TCCGGA T^CCGGA BsiWI 438 CGTACG C^GTACG BspDI 899 ATCGAT AT^CGAT BspEI 181 TCCGGA T^CCGGA BspHI 1307 TCATGA T^CATGA BsrDI 289 GCAATG GCAATG(2/0) BsrFI 375 ACCGGT [GA]^CCGG[CT] BstAPI 264 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 240 TTCGAA TT^CGAA BstYI 830 GGATCT [GA]^GATC[CT] CfoI 1318 GCGC GCG^C Cfr10I 375 ACCGGT [GA]^CCGG[CT] ClaI 899 ATCGAT AT^CGAT DraII 314 AGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 23 CGGCCA [CT]^GGCC[GA] Eam1105I 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 314 AGGGCCC [GA]G^G[ACGT]CC[CT] HhaI 1318 GCGC GCG^C Hin1I 1290 GACGCC G[GA]^CG[CT]C HinP1I 1318 GCGC G^CGC KpnI 378 GGTACC GGTAC^C KspI 851 CCGCGG CCGC^GG MaeIII 1000 GTTAC ^GT[ACGT]AC MflI 830 GGATCT [GA]^GATC[CT] MroI 181 TCCGGA T^CCGGA MslI 1221 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 851 CCGCGG C[AC]G^C[GT]G NspV 240 TTCGAA TT^CGAA PinAI 375 ACCGGT A^CCGGT PmaCI 60 CACGTG CAC^GTG PmlI 60 CACGTG CAC^GTG PshBI 1059 ATTAAT AT^TAAT PstI 628 CTGCAG CTGCA^G RcaI 1307 TCATGA T^CATGA SacII 851 CCGCGG CCGC^GG ScaI 1485 AGTACT AGT^ACT SfcI 628 CTGCAG C^T[GA][CT]AG SfuI 240 TTCGAA TT^CGAA SmaI 1136 CCCGGG CCC^GGG SnaBI 544 TACGTA TAC^GTA SpeI 1482 ACTAGT A^CTAGT SphI 307 GCATGC GCATG^C XhoII 830 GGATCT [GA]^GATC[CT] XmaCI 1136 CCCGGG C^CCGGG XmaI 1136 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 852 CGCG CG^CG 1317 CGCG AvaI 586 CCCGAG C^[CT]CG[GA]G 1136 CCCGGG BanI 187 GGCACC G^G[CT][GA]CC 378 GGTACC BcnI 1136 CCCGG CC^[GC]GG 1137 CCGGG BpmI 1094 CTCCAG CTGGAG(16/14) 116 CTGGAG BsaAI 60 CACGTG [CT]AC^GT[GA] 544 TACGTA BsaWI 181 TCCGGA [AT]^CCGG[AT] 375 ACCGGT BseRI 85 GAGGAG GAGGAG(10/8) 88 GAGGAG BsmBI 91 GAGACG CGTCTC(1/5) 1288 GAGACG BsoBI 586 CCCGAG C^[CT]CG[GA]G 1136 CCCGGG Bsp1407I 845 TGTACA T^GTACA 1439 TGTACA BsrGI 845 TGTACA T^GTACA 1439 TGTACA BstUI 852 CGCG CG^CG 1317 CGCG BstXI 613 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 914 CCAAAAAATTGG DdeI 869 CTAAG C^T[ACGT]AG 1198 CTGAG DsaI 851 CCGCGG C^C[GA][CT]GG 1220 CCACGG EcoRV 277 GATATC GAT^ATC 1039 GATATC FauI 65 GCGGG CCCGC(4/6) 853 GCGGG HaeIII 24 GGCC GG^CC 316 GGCC HphI 1224 GGTGA GGTGA(8/7) 583 TCACC MfeI 416 CAATTG C^AATTG 1443 CAATTG MunI 416 CAATTG C^AATTG 1443 CAATTG MvnI 852 CGCG CG^CG 1317 CGCG NciI 1136 CCCGG CC^[GC]GG 1137 CCGGG NspI 307 GCATGC [GA]CATG^[CT] 883 ACATGC PleI 199 GACTC GAGTC(4/5) 16 GAGTC SspBI 845 TGTACA T^GTACA 1439 TGTACA TfiI 211 GATTC G^A[AT]TC 1465 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI BanII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI FokI HapII HgaI HinfI HpaII ItaI Ksp632I MaeI MaeII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I