(Circular) (Six-base) MAPSORT of: pFN21AE4481.seq Check: 5656 from: 1 to: 6199 pFN21AE4481 6199 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3542 1937 Size: 1605 4594 Acc65I G'GTAC_C Cuts at: 2383 3520 2383 Size: 1137 5062 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3000 3101 4262 5646 5914 1632 Size: 1368 101 1161 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1161 268 101 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 2064 2529 3159 5098 6075 1203 Size: 861 465 630 1939 977 1327 Fragments arranged by size: 1939 1327 977 861 630 465 AgeI A'CCGG_T Cuts at: 2380 5030 2380 Size: 2650 3549 AhdI GACnn_n'nnGTC Cuts at: 2020 4928 2020 Size: 2908 3291 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3258 1184 Size: 2074 4125 AlwNI CAG_nnn'CTG Cuts at: 2044 2898 3473 3942 5514 2044 Size: 854 575 469 1572 2729 Fragments arranged by size: 2729 1572 854 575 469 ApaI G_GGCC'C Cuts at: 2324 2324 Size: 6199 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2182 2232 2939 3508 3734 3886 5995 1493 Size: 689 50 707 569 226 152 2109 1697 Fragments arranged by size: 2109 1697 707 689 569 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 3065 4753 160 Size: 872 2033 1688 1606 Fragments arranged by size: 2033 1688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 2591 3141 3524 1870 Size: 73 648 550 383 4545 Fragments arranged by size: 4545 648 550 383 73 BamHI G'GATC_C Cuts at: 3529 3896 3529 Size: 367 5832 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2383 3520 4975 618 Size: 325 217 627 405 191 1137 1455 1842 Fragments arranged by size: 1842 1455 1137 627 405 325 217 191 BanII G_rGCy'C Cuts at: 1391 2324 2611 3179 3518 1391 Size: 933 287 568 339 4072 Fragments arranged by size: 4072 933 568 339 287 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2652 928 Size: 971 753 4475 Fragments arranged by size: 4475 971 753 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4390 4424 1903 Size: 34 2453 34 3678 Fragments arranged by size: 3678 2453 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2324 4260 5416 1790 Size: 534 1936 1156 2573 Fragments arranged by size: 2573 1936 1156 534 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2250 2786 3930 Size: 416 53 83 399 24 833 536 1144 Cuts at: 3930 4888 442 Size: 958 1753 Fragments arranged by size: 1753 1144 958 833 536 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 3084 4859 1239 Size: 903 942 1775 2579 Fragments arranged by size: 2579 1775 942 903 Bpu10I CC'TnA_GC Cuts at: 2874 3203 2874 Size: 329 5870 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 2518 2971 3389 4324 5189 5487 2501 Size: 17 453 418 935 865 298 3213 Fragments arranged by size: 3213 935 865 453 418 298 17 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3019 4862 882 Size: 617 1520 1843 2219 Fragments arranged by size: 2219 1843 1520 617 BsaAI yAC'GTr Cuts at: 493 1204 2067 2551 3990 493 Size: 711 863 484 1439 2702 Fragments arranged by size: 2702 1439 863 711 484 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3296 4006 4388 5023 Size: 53 83 186 936 1763 710 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1763 1451 936 710 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2186 2380 4632 5030 5304 5451 5862 2186 Size: 194 2252 398 274 147 411 2523 Fragments arranged by size: 2523 2252 411 398 274 194 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2108 1186 Size: 919 3 5277 Fragments arranged by size: 5277 919 3 BseYI C'CCAG_C Cuts at: 2323 2698 5402 5898 2323 Size: 375 2704 496 2624 Fragments arranged by size: 2704 2624 496 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5626 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2517 3150 4260 4345 5416 2106 Size: 411 633 1110 85 1071 2889 Fragments arranged by size: 2889 1110 1071 633 411 85 BsiWI C'GTAC_G Cuts at: 2443 2443 Size: 6199 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 3287 2090 Size: 1197 5002 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2324 2517 2611 3150 3179 3518 Size: 399 316 218 193 94 539 29 339 Cuts at: 3518 4260 4345 5416 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 539 399 339 316 218 193 94 85 29 BspEI T'CCGG_A Cuts at: 2186 5862 2186 Size: 3676 2523 BspHI T'CATG_A Cuts at: 3312 4088 5818 3312 Size: 776 1730 3693 Fragments arranged by size: 3693 1730 776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 844 Size: 2710 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2301 4694 4868 65 Size: 1183 1053 2393 174 1396 Fragments arranged by size: 2393 1396 1183 1053 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2380 4843 5030 1700 Size: 110 570 2463 187 2869 Fragments arranged by size: 2869 2463 570 187 110 BsrGI T'GTAC_A Cuts at: 96 2850 3444 96 Size: 2754 594 2851 Fragments arranged by size: 2851 2754 594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 2275 794 Size: 1481 4718 BstBI TT'CG_AA Cuts at: 2246 5003 2246 Size: 2757 3442 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstXI CCAn_nnnn'nTGG Cuts at: 2625 2926 2625 Size: 301 5898 BstYI r'GATC_y Cuts at: 1972 2835 3529 3896 4281 4298 5750 5836 5848 Size: 863 694 367 385 17 1452 86 12 Cuts at: 5848 6194 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 863 694 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2856 3225 513 Size: 552 939 852 369 3487 Fragments arranged by size: 3487 939 852 552 369 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 2642 3715 4509 4529 2642 Size: 1073 794 20 4312 Fragments arranged by size: 4312 1073 794 20 ClaI AT'CG_AT Cuts at: 1554 2905 1554 Size: 1351 4848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 3646 4535 Size: 54 1425 50 275 18 198 1618 889 Cuts at: 4535 6164 8 Size: 1629 43 Fragments arranged by size: 1629 1618 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 2812 3108 4129 1353 Size: 803 656 296 1021 3423 Fragments arranged by size: 3423 1021 803 656 296 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4786 5160 5306 387 Size: 1064 138 435 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 3000 3084 3101 4262 4859 5646 Size: 393 510 858 84 17 1161 597 787 Cuts at: 5646 5914 1239 Size: 268 1524 Fragments arranged by size: 1524 1161 858 787 597 510 393 268 84 17 EcoO109I rG'GnC_Cy Cuts at: 2320 2320 Size: 6199 EcoRI G'AATT_C Cuts at: 3508 3886 3508 Size: 378 5821 EcoRV GAT'ATC Cuts at: 2284 3046 2284 Size: 762 5437 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3409 3409 Size: 6199 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 5346 1678 Size: 3668 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2195 2227 2291 Size: 32 135 32 434 32 157 32 64 Cuts at: 2291 2323 3035 3051 3067 3083 4840 4872 4914 Size: 32 712 16 16 16 1757 32 42 Cuts at: 4914 4946 1373 Size: 32 2626 Fragments arranged by size: 2626 1757 712 434 157 135 64 42 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 2387 3524 2387 Size: 1137 5062 MfeI C'AATT_G Cuts at: 2421 3448 3798 2421 Size: 1027 350 4822 Fragments arranged by size: 4822 1027 350 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3463 5313 5497 1305 Size: 2158 1850 184 2007 Fragments arranged by size: 2158 2007 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4377 Fragments arranged by size: 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3230 4157 4516 4675 518 Size: 648 2064 927 359 159 2042 Fragments arranged by size: 2064 2042 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2858 3615 4292 5440 5685 1606 Size: 1252 757 677 1148 245 2120 Fragments arranged by size: 2120 1252 1148 757 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4708 Fragments arranged by size: 4708 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 2316 2892 3557 5102 1132 Size: 97 1087 576 665 1545 2229 Fragments arranged by size: 2229 1545 1087 665 576 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PfoI T'CCnGG_A Cuts at: 2119 2119 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PmlI CAC'GTG Cuts at: 2067 2067 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 2320 2320 Size: 6199 PstI C_TGCA'G Cuts at: 830 2637 3551 830 Size: 1807 914 3478 Fragments arranged by size: 3478 1807 914 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 SacII CC_GC'GG Cuts at: 2859 2859 Size: 6199 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 3492 4447 6159 3492 Size: 955 1712 3532 Fragments arranged by size: 3532 1712 955 SfcI C'TryA_G Cuts at: 826 1045 2633 3547 4682 5363 5554 826 Size: 219 1588 914 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 914 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3143 3526 1872 Size: 1271 383 4545 Fragments arranged by size: 4545 1271 383 SmlI C'TyrA_G Cuts at: 820 1017 1943 2497 2516 2950 3404 4303 5204 Size: 197 926 554 19 434 454 899 901 Cuts at: 5204 5466 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 899 554 454 434 262 197 19 SnaBI TAC'GTA Cuts at: 493 2551 3990 493 Size: 2058 1439 2702 Fragments arranged by size: 2702 2058 1439 SpeI A'CTAG_T Cuts at: 152 3487 152 Size: 3335 2864 SphI G_CATG'C Cuts at: 1132 2316 3557 1132 Size: 1184 1241 3774 Fragments arranged by size: 3774 1241 1184 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 2004 3638 513 Size: 552 939 1634 3074 Fragments arranged by size: 3074 1634 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4226 4243 4396 4581 5026 325 Size: 1255 2646 17 153 185 445 1498 Fragments arranged by size: 2646 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2850 3444 3490 Size: 274 80 33 51 1443 873 594 46 Cuts at: 3490 4445 6157 96 Size: 955 1712 138 Fragments arranged by size: 1712 1443 955 873 594 274 138 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 6159 6191 2580 Size: 32 3547 32 2588 Fragments arranged by size: 3547 2588 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3141 3524 1870 Size: 1271 383 4545 Fragments arranged by size: 4545 1271 383 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI