Restriction map for
pFN21AE4481
(Linear) (Six-base) MAPSORT of: KIEE4481.seq Check: 8197 from: 1 to: 1494
KIEE4481.orf
With 182 enzymes: SgfI *
March 22, 2012 18:10 ..
Acc65I G'GTAC_C
Cuts at: 0 378 1494
Size: 378 1116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1096 1494
Size: 995 101 398
Fragments arranged by size:
995 398 101
AflIII A'CryG_T
Cuts at: 0 59 524 1154 1494
Size: 59 465 630 340
Fragments arranged by size:
630 465 340 59
AgeI A'CCGG_T
Cuts at: 0 375 1494
Size: 375 1119
AhdI GACnn_n'nnGTC
Cuts at: 0 15 1494
Size: 15 1479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1253 1494
Size: 1253 241
AlwNI CAG_nnn'CTG
Cuts at: 0 39 893 1468 1494
Size: 39 854 575 26
Fragments arranged by size:
854 575 39 26
ApaI G_GGCC'C
Cuts at: 0 319 1494
Size: 319 1175
ApoI r'AATT_y
Cuts at: 0 177 227 934 1494
Size: 177 50 707 560
Fragments arranged by size:
707 560 177 50
AseI AT'TA_AT
Cuts at: 0 1060 1494
Size: 1060 434
AvaI C'yCGr_G
Cuts at: 0 586 1136 1494
Size: 586 550 358
Fragments arranged by size:
586 550 358
BanI G'GyrC_C
Cuts at: 0 187 378 1494
Size: 187 191 1116
Fragments arranged by size:
1116 191 187
BanII G_rGCy'C
Cuts at: 0 319 606 1174 1494
Size: 319 287 568 320
Fragments arranged by size:
568 320 319 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 647 1494
Size: 647 847
Bme1580I G_kGCm'C
Cuts at: 0 319 1494
Size: 319 1175
BmrI ACTGGGnnnn_n'
Cuts at: 0 245 781 1494
Size: 245 536 713
Fragments arranged by size:
713 536 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1079 1494
Size: 137 942 415
Fragments arranged by size:
942 415 137
Bpu10I CC'TnA_GC
Cuts at: 0 869 1198 1494
Size: 869 329 296
Fragments arranged by size:
869 329 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 513 966 1384 1494
Size: 496 17 453 418 110
Fragments arranged by size:
496 453 418 110 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 1014 1494
Size: 1014 480
BsaAI yAC'GTr
Cuts at: 0 62 546 1494
Size: 62 484 948
Fragments arranged by size:
948 484 62
BsaHI Gr'CG_yC
Cuts at: 0 1291 1494
Size: 1291 203
BsaWI w'CCGG_w
Cuts at: 0 181 375 1494
Size: 181 194 1119
Fragments arranged by size:
1119 194 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 103 1494
Size: 100 3 1391
Fragments arranged by size:
1391 100 3
BseYI C'CCAG_C
Cuts at: 0 318 693 1494
Size: 318 375 801
Fragments arranged by size:
801 375 318
BsiHKAI G_wGCw'C
Cuts at: 0 101 512 1145 1494
Size: 101 411 633 349
Fragments arranged by size:
633 411 349 101
BsiWI C'GTAC_G
Cuts at: 0 438 1494
Size: 438 1056
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 1282 1494
Size: 85 1197 212
Fragments arranged by size:
1197 212 85
Bsp1286I G_dGCh'C
Cuts at: 0 101 319 512 606 1145 1174 1494
Size: 101 218 193 94 539 29 320
Fragments arranged by size:
539 320 218 193 101 94 29
BspEI T'CCGG_A
Cuts at: 0 181 1494
Size: 181 1313
BspHI T'CATG_A
Cuts at: 0 1307 1494
Size: 1307 187
BsrDI GCAATG_nn'
Cuts at: 0 296 1494
Size: 296 1198
BsrFI r'CCGG_y
Cuts at: 0 375 1494
Size: 375 1119
BsrGI T'GTAC_A
Cuts at: 0 845 1439 1494
Size: 845 594 55
Fragments arranged by size:
845 594 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 1494
Size: 270 1224
BstBI TT'CG_AA
Cuts at: 0 241 1494
Size: 241 1253
BstXI CCAn_nnnn'nTGG
Cuts at: 0 620 921 1494
Size: 620 301 573
Fragments arranged by size:
620 573 301
BstYI r'GATC_y
Cuts at: 0 830 1494
Size: 830 664
BtgI C'CryG_G
Cuts at: 0 851 1220 1494
Size: 851 369 274
Fragments arranged by size:
851 369 274
BtsI GCAGTG_nn'
Cuts at: 0 637 1494
Size: 637 857
ClaI AT'CG_AT
Cuts at: 0 900 1494
Size: 900 594
EaeI y'GGCC_r
Cuts at: 0 23 1494
Size: 23 1471
EarI CTCTTCn'nnn_
Cuts at: 0 151 807 1103 1494
Size: 151 656 296 391
Fragments arranged by size:
656 391 296 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1494
Size: 19 1475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 995 1079 1096 1494
Size: 137 858 84 17 398
Fragments arranged by size:
858 398 137 84 17
EcoO109I rG'GnC_Cy
Cuts at: 0 315 1494
Size: 315 1179
EcoRV GAT'ATC
Cuts at: 0 279 1041 1494
Size: 279 762 453
Fragments arranged by size:
762 453 279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1404 1494
Size: 1404 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 190 222 286 318 1030 1046
Size: 1 32 157 32 64 32 712 16
Cuts at: 1046 1062 1078 1494
Size: 16 16 416
Fragments arranged by size:
712 416 157 64 32 32 32 16
16 16 1
KpnI G_GTAC'C
Cuts at: 0 382 1494
Size: 382 1112
MfeI C'AATT_G
Cuts at: 0 416 1443 1494
Size: 416 1027 51
Fragments arranged by size:
1027 416 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1458 1494
Size: 1458 36
MslI CAynn'nnrTG
Cuts at: 0 1225 1494
Size: 1225 269
MspA1I CmG'CkG
Cuts at: 0 853 1494
Size: 853 641
NspI r_CATG'y
Cuts at: 0 311 887 1494
Size: 311 576 607
Fragments arranged by size:
607 576 311
PfoI T'CCnGG_A
Cuts at: 0 114 1494
Size: 114 1380
PmlI CAC'GTG
Cuts at: 0 62 1494
Size: 62 1432
PspOMI G'GGCC_C
Cuts at: 0 315 1494
Size: 315 1179
PstI C_TGCA'G
Cuts at: 0 632 1494
Size: 632 862
SacII CC_GC'GG
Cuts at: 0 854 1494
Size: 854 640
ScaI AGT'ACT
Cuts at: 0 1487 1494
Size: 1487 7
SfcI C'TryA_G
Cuts at: 0 628 1494
Size: 628 866
SmaI CCC'GGG
Cuts at: 0 1138 1494
Size: 1138 356
SmlI C'TyrA_G
Cuts at: 0 492 511 945 1399 1494
Size: 492 19 434 454 95
Fragments arranged by size:
492 454 434 95 19
SnaBI TAC'GTA
Cuts at: 0 546 1494
Size: 546 948
SpeI A'CTAG_T
Cuts at: 0 1482 1494
Size: 1482 12
SphI G_CATG'C
Cuts at: 0 311 1494
Size: 311 1183
TatI w'GTAC_w
Cuts at: 0 845 1439 1485 1494
Size: 845 594 46 9
Fragments arranged by size:
845 594 46 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 1494
Size: 575 32 887
Fragments arranged by size:
887 575 32
XmaI C'CCGG_G
Cuts at: 0 1136 1494
Size: 1136 358
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AhdI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrDI
BsrFI BsrGI BstAPI BstBI BstXI BstYI BtgI BtsI
ClaI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI
Hin4I KpnI MfeI MmeI MslI MspA1I NspI PfoI
PmlI PspOMI PstI SacII ScaI SfcI SmaI SmlI
SnaBI SpeI SphI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BsaBI BsaXI BsgI BsiEI BsmI BspMI
BsrBI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XhoI XmnI ZraI