(Circular) (Six-base) MAPSORT of: pFN21AE5208.seq Check: 5470 from: 1 to: 5437 pFN21AE5208 5437 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 3500 4884 5152 1632 Size: 767 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 2304 4336 5313 1203 Size: 1101 2032 977 1327 Fragments arranged by size: 2032 1327 1101 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 2449 2496 3180 4752 2449 Size: 47 684 1572 3134 Fragments arranged by size: 3134 1572 684 47 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2310 2762 1870 Size: 73 367 452 4545 Fragments arranged by size: 4545 452 367 73 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2279 2534 2666 2758 Size: 325 217 627 239 253 255 132 92 Cuts at: 2758 4213 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 255 253 239 217 132 92 BanII G_rGCy'C Cuts at: 1391 2231 2422 2756 1391 Size: 840 191 334 4072 Fragments arranged by size: 4072 840 334 191 BbeI G_GCGC'C Cuts at: 2283 2670 2283 Size: 387 5050 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2374 2408 3628 3662 1903 Size: 34 437 34 1220 34 3678 Fragments arranged by size: 3678 1220 437 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2421 2854 2421 Size: 433 5004 Bme1580I G_kGCm'C Cuts at: 1790 2537 3498 4654 1790 Size: 747 961 1156 2573 Fragments arranged by size: 2573 1156 961 747 BmgBI CAC'GTC Cuts at: 1318 2263 2305 1318 Size: 945 42 4450 Fragments arranged by size: 4450 945 42 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2615 3168 4126 Size: 416 53 83 399 24 1198 553 958 Cuts at: 4126 442 Size: 1753 Fragments arranged by size: 1753 1198 958 553 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2186 4097 1239 Size: 908 39 1911 2579 Fragments arranged by size: 2579 1911 908 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 4100 882 Size: 617 1059 1542 2219 Fragments arranged by size: 2219 1542 1059 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 2667 3244 3626 Size: 53 83 186 936 747 387 577 382 Cuts at: 3626 4261 275 Size: 635 1451 Fragments arranged by size: 1451 936 747 635 577 387 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2426 2471 2726 1186 Size: 1237 3 45 255 3897 Fragments arranged by size: 3897 1237 255 45 3 BseYI C'CCAG_C Cuts at: 2442 4640 5136 2442 Size: 2198 496 2743 Fragments arranged by size: 2743 2198 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4864 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2398 2887 3648 4676 1540 Size: 275 186 397 489 761 1028 2301 Fragments arranged by size: 2301 1028 761 489 397 275 186 BsiHKAI G_wGCw'C Cuts at: 3498 3583 4654 3498 Size: 85 1071 4281 Fragments arranged by size: 4281 1071 85 BsmI GAATG_Cn' Cuts at: 2654 2948 3041 2654 Size: 294 93 5050 Fragments arranged by size: 5050 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2422 2537 2756 3498 3583 4654 Size: 399 441 191 115 219 742 85 1071 Cuts at: 4654 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 441 399 219 191 115 85 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2792 844 Size: 1948 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2073 2370 2416 3324 1394 Size: 429 250 297 46 908 3507 Fragments arranged by size: 3507 908 429 297 250 46 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2397 4081 4268 1700 Size: 110 587 1684 187 2869 Fragments arranged by size: 2869 1684 587 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 2646 4241 2646 Size: 1595 3842 BstEII G'GTnAC_C Cuts at: 1184 2035 1184 Size: 851 4586 BstXI CCAn_nnnn'nTGG Cuts at: 2485 2485 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432 Size: 795 367 385 17 1452 86 12 346 Cuts at: 5432 1972 Size: 1977 Fragments arranged by size: 1977 1452 795 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2484 513 Size: 552 939 27 453 3466 Fragments arranged by size: 3466 939 552 453 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 487 Size: 683 56 863 3835 Fragments arranged by size: 3835 863 683 56 BtsI GCAGTG_nn' Cuts at: 2629 2953 3747 3767 2629 Size: 324 794 20 4299 Fragments arranged by size: 4299 794 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2020 4444 809 Size: 507 704 2424 1802 Fragments arranged by size: 2424 1802 704 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2395 2451 2884 Size: 54 1425 50 275 18 565 56 433 Cuts at: 2884 3773 5402 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 565 433 275 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2395 2884 1537 Size: 275 583 489 4090 Fragments arranged by size: 4090 583 489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3367 1353 Size: 2014 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2186 2399 3500 4097 4884 5152 Size: 393 515 39 213 1101 597 787 268 Cuts at: 5152 1239 Size: 1524 Fragments arranged by size: 1524 1101 787 597 515 393 268 213 39 EcoNI CCTnn'n_nnAGG Cuts at: 2453 2453 Size: 5437 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2283 2449 2670 4584 1678 Size: 605 166 221 1914 2531 Fragments arranged by size: 2531 1914 605 221 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 4078 4110 4152 Size: 32 135 32 719 32 1755 32 42 Cuts at: 4152 4184 1373 Size: 32 2626 Fragments arranged by size: 2626 1755 719 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2781 3027 669 Size: 988 281 244 599 246 3079 Fragments arranged by size: 3079 988 599 281 246 244 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KasI G'GCGC_C Cuts at: 2279 2666 2279 Size: 387 5050 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 4551 4735 1305 Size: 1401 1845 184 2007 Fragments arranged by size: 2007 1845 1401 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2483 2540 3395 3754 3913 518 Size: 648 843 474 57 855 359 159 2042 Fragments arranged by size: 2042 855 843 648 474 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2496 2853 3530 4678 4923 1606 Size: 890 357 677 1148 245 2120 Fragments arranged by size: 2120 1148 890 677 357 245 NarI GG'CG_CC Cuts at: 2280 2667 2280 Size: 387 5050 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2795 4340 1132 Size: 97 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 1108 Size: 1341 4096 PfoI T'CCnGG_A Cuts at: 2706 2706 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PstI C_TGCA'G Cuts at: 830 2789 830 Size: 1959 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 RsrII CG'GwC_CG Cuts at: 2365 2365 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2194 2238 2785 3920 4601 4792 826 Size: 219 1149 44 547 1135 681 191 1471 Fragments arranged by size: 1471 1149 1135 681 547 219 191 44 SfoI GGC'GCC Cuts at: 2281 2668 2281 Size: 387 5050 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2312 2764 1872 Size: 440 452 4545 Fragments arranged by size: 4545 452 440 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5437 StyI C'CwwG_G Cuts at: 513 1065 2004 2287 2876 513 Size: 552 939 283 589 3074 Fragments arranged by size: 3074 939 589 552 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 3464 3481 3634 3819 4264 325 Size: 1255 464 1420 17 153 185 445 1498 Fragments arranged by size: 1498 1420 1255 464 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3683 5395 Size: 274 80 33 51 1443 345 1361 1712 Cuts at: 5395 96 Size: 138 Fragments arranged by size: 1712 1443 1361 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 2363 1316 Size: 1047 4390 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2660 2660 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2310 2762 1870 Size: 440 452 4545 Fragments arranged by size: 4545 452 440 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI