################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 872 GTCTAC GT^[AC][GT]AC AccII 1098 CGCG CG^CG AcsI 289 AAATTT [GA]^AATT[CT] AlwI 1366 GATCC GGATC(4/5) ApoI 289 AAATTT [GA]^AATT[CT] Asp700I 226 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1396 GGACC G^G[AT]CC AvrII 1122 CCTAGG C^CTAGG BanI 649 GGCACC G^G[CT][GA]CC BbvI 619 GCTGC GCAGC(8/12) BcnI 710 CCCGG CC^[GC]GG BfaI 1123 CTAG C^TAG BlnI 1122 CCTAGG C^CTAGG BsaBI 679 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiYI 369 CCAATGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 369 CCAATGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 615 GAATGC GAATGC(1/-1) BspHI 659 TCATGA T^CATGA BsrBI 1073 CCGCTC CCGCTC(-3/-3) BsrDI 731 GCAATG GCAATG(2/0) BsrI 1374 ACTGG ACTGG(1/-1) BstUI 1098 CGCG CG^CG BstYI 1365 AGATCC [GA]^GATC[CT] Cfr13I 1396 GGACC G^G[ACGT]CC FauI 1072 CCCGC CCCGC(4/6) HapII 711 CCGG C^CGG HgaI 421 GACGC GACGC(5/10) HpaII 711 CCGG C^CGG MaeI 1123 CTAG C^TAG MaeII 1279 ACGT A^CGT MamI 679 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1365 AGATCC [GA]^GATC[CT] MseI 1028 TTAA T^TAA MspA1I 749 CAGCTG C[AC]G^C[GT]G MspI 711 CCGG C^CGG MvnI 1098 CGCG CG^CG MwoI 1184 GCTTGTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 710 CCCGG CC^[GC]GG PshAI 321 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 749 CAGCTG CAG^CTG RcaI 659 TCATGA T^CATGA Sau96I 1396 GGACC G^G[ACGT]CC SfcI 902 CTACAG C^T[GA][CT]AG Tru9I 1028 TTAA T^TAA TseI 619 GCTGC G^C[AT]GC XcmI 795 CCATGAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1365 AGATCC [GA]^GATC[CT] XmnI 226 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 736 GTAC GT^AC 847 GTAC AspHI 913 GAGCTC G[AT]GC[AT]^C 1312 GAGCTC BalI 366 TGGCCA TGG^CCA 441 TGGCCA BsaI 1047 GGTCTC GGTCTC(1/5) 1108 GGTCTC BseRI 113 GAGGAG GAGGAG(10/8) 955 GAGGAG BsiHKAI 913 GAGCTC G[AT]GC[AT]^C 1312 GAGCTC DpnII 295 GATC ^GATC 1366 GATC EcoT14I 688 CCTTGG C^C[AT][AT]GG 1122 CCTAGG Fnu4HI 518 GCCGC GC^[ACGT]GC 619 GCTGC HincII 1089 GTCAAC GT[CT]^[GA]AC 1297 GTTGAC HindII 1089 GTCAAC GT[CT]^[GA]AC 1297 GTTGAC ItaI 518 GCCGC GC^[ACGT]GC 619 GCTGC MboI 295 GATC ^GATC 1366 GATC MluNI 366 TGGCCA TGG^CCA 441 TGGCCA MscI 366 TGGCCA TGG^CCA 441 TGGCCA NdeII 295 GATC ^GATC 1366 GATC PleI 694 GACTC GAGTC(4/5) 1385 GACTC RsaI 736 GTAC GT^AC 847 GTAC SacI 913 GAGCTC GAGCT^C 1312 GAGCTC Sau3AI 295 GATC ^GATC 1366 GATC SfaNI 531 GCATC GCATC(5/9) 557 GCATC SmlI 76 CTTGAG C^T[CT][GA]AG 811 CTTGAG SspI 266 AATATT AAT^ATT 506 AATATT StyI 688 CCTTGG C^C[AT][AT]GG 1122 CCTAGG TfiI 780 GAATC G^A[AT]TC 1018 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI BanII BmyI BsaJI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I DdeI EaeI EcoRII FokI HaeIII HinfI HphI MaeIII MboII MnlI MslI MvaI NlaIII NlaIV ScrFI Tsp45I Tsp509I TspRI