(Circular) (Six-base) MAPSORT of: pFN21AE6409.seq Check: 2416 from: 1 to: 6118 pFN21AE6409 6118 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2878 3461 1937 Size: 941 583 4594 Fragments arranged by size: 4594 941 583 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2127 2250 2495 2700 4181 5565 5833 Size: 446 49 123 245 205 1481 1384 268 Cuts at: 5833 1632 Size: 1917 Fragments arranged by size: 1917 1481 1384 446 268 245 205 123 49 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2759 1184 Size: 1575 4543 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2690 2722 2690 Size: 32 6086 AlwNI CAG_nnn'CTG Cuts at: 3861 5433 3861 Size: 1572 4546 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2294 3427 3653 3805 5914 1493 Size: 801 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 801 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 AvrII C'CTAG_G Cuts at: 3127 3127 Size: 6118 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2654 3439 4894 618 Size: 325 217 627 867 785 1455 1842 Fragments arranged by size: 1842 1455 867 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2922 3321 3437 1391 Size: 697 834 399 116 4072 Fragments arranged by size: 4072 834 697 399 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 4179 5335 1790 Size: 2389 1156 2573 Fragments arranged by size: 2573 2389 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4778 1239 Size: 3539 2579 Bpu10I CC'TnA_GC Cuts at: 2628 2628 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 2837 4243 5108 5406 2102 Size: 735 1406 865 298 2814 Fragments arranged by size: 2814 1406 865 735 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3058 3119 4781 882 Size: 617 1559 61 1662 2219 Fragments arranged by size: 2219 1662 1559 617 61 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 2688 6112 2688 Size: 3424 2694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 1178 Size: 30 165 30 619 30 5244 Fragments arranged by size: 5244 619 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2133 2975 1186 Size: 947 842 4329 Fragments arranged by size: 4329 947 842 BseYI C'CCAG_C Cuts at: 2033 5321 5817 2033 Size: 3288 496 2334 Fragments arranged by size: 3288 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5545 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2922 3321 4179 4264 5335 2922 Size: 399 858 85 1071 3705 Fragments arranged by size: 3705 1071 858 399 85 BsmI GAATG_Cn' Cuts at: 2626 3629 3722 2626 Size: 1003 93 5022 Fragments arranged by size: 5022 1003 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2922 3321 3437 4179 4264 5335 Size: 399 298 834 399 116 742 85 1071 Cuts at: 5335 1391 Size: 2174 Fragments arranged by size: 2174 1071 834 742 399 399 298 116 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 2664 4007 5737 2664 Size: 1343 1730 3045 Fragments arranged by size: 3045 1730 1343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3080 4005 1394 Size: 429 1257 925 3507 Fragments arranged by size: 3507 1257 925 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2743 4613 4787 65 Size: 1183 1495 1870 174 1396 Fragments arranged by size: 1870 1495 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3370 3448 3815 4200 4217 5669 5755 5767 Size: 1398 78 367 385 17 1452 86 12 Cuts at: 5767 6113 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1398 385 367 346 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2449 3634 4428 4448 2449 Size: 1185 794 20 4119 Fragments arranged by size: 4119 1185 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 529 Size: 35 1513 35 4535 Fragments arranged by size: 4535 1513 35 35 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 2371 2446 Size: 54 1425 50 275 18 322 219 75 Cuts at: 2446 3565 4454 6083 8 Size: 1119 889 1629 43 Fragments arranged by size: 1629 1425 1119 889 322 275 219 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4048 1353 Size: 2695 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2920 3319 2920 Size: 399 5719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2127 2250 2495 2700 4181 4778 Size: 393 446 49 123 245 205 1481 597 Cuts at: 4778 5565 5833 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1481 787 597 446 393 268 245 205 123 49 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2148 2180 2335 Size: 32 135 32 450 32 94 32 155 Cuts at: 2335 2348 2367 2380 3338 3370 4759 4791 4833 Size: 13 19 13 958 32 1389 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 1389 958 450 155 135 94 42 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3304 3462 3708 669 Size: 988 281 1158 208 158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 208 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2373 2448 10 Size: 54 1425 343 541 75 3680 Fragments arranged by size: 3680 1425 541 343 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 2567 2897 4076 4435 4594 518 Size: 648 1375 26 330 1179 359 159 2042 Fragments arranged by size: 2042 1375 1179 648 359 330 159 26 MspA1I CmG'CkG Cuts at: 1606 2756 3534 4211 5359 5604 1606 Size: 1150 778 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 778 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2330 1613 Size: 717 5401 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2756 2756 Size: 6118 SacI G_AGCT'C Cuts at: 2922 3321 2922 Size: 399 5719 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2907 3466 4601 5282 5473 826 Size: 219 1862 559 1135 681 191 1471 Fragments arranged by size: 1862 1471 1135 681 559 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 2816 4222 5123 5385 820 Size: 197 926 138 735 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 735 262 197 138 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 2273 2513 4042 5 Size: 47 2221 240 1529 2081 Fragments arranged by size: 2221 2081 1529 240 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2004 2693 3127 3557 513 Size: 552 939 689 434 430 3074 Fragments arranged by size: 3074 939 689 552 434 430 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3157 4145 4162 4315 4500 4945 325 Size: 1255 1577 988 17 153 185 445 1498 Fragments arranged by size: 1577 1498 1255 988 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2740 2851 4364 Size: 274 80 33 51 1443 763 111 1513 Cuts at: 4364 6076 96 Size: 1712 138 Fragments arranged by size: 1712 1513 1443 763 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2807 2807 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 2235 4247 2235 Size: 2012 4106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI