(Circular) (Six-base) MAPSORT of: pFN21AE0099.seq Check: 40 from: 1 to: 5506 pFN21AE0099 5506 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3316 278 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2230 3569 4953 5221 1632 Size: 598 1339 1384 268 1917 Fragments arranged by size: 1917 1384 1339 598 268 AfeI AGC'GCT Cuts at: 2594 2770 2594 Size: 176 5330 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 2164 4235 2164 Size: 2071 3435 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AlwNI CAG_nnn'CTG Cuts at: 2476 3249 4821 2476 Size: 773 1572 3161 Fragments arranged by size: 3161 1572 773 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2815 3041 3193 5302 1493 Size: 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2708 2774 2831 1870 Size: 73 765 66 57 4545 Fragments arranged by size: 4545 765 73 66 57 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2633 2827 4282 618 Size: 325 217 627 846 194 1455 1842 Fragments arranged by size: 1842 1455 846 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2096 2418 2454 2504 2825 1391 Size: 705 322 36 50 321 4072 Fragments arranged by size: 4072 705 322 321 50 36 BbeI G_GCGC'C Cuts at: 2637 2637 Size: 5506 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3697 3731 1903 Size: 34 1760 34 3678 Fragments arranged by size: 3678 1760 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2508 2642 4117 Size: 107 122 71 631 605 836 134 1475 Cuts at: 4117 136 Size: 1525 Fragments arranged by size: 1525 1475 836 631 605 134 122 107 71 BglII A'GATC_T Cuts at: 5501 5501 Size: 5506 BlpI GC'TnA_GC Cuts at: 2498 2684 2923 2498 Size: 186 239 5081 Fragments arranged by size: 5081 239 186 Bme1580I G_kGCm'C Cuts at: 1790 3567 4723 1790 Size: 1777 1156 2573 Fragments arranged by size: 2573 1777 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2164 2233 2516 Size: 416 53 83 399 24 747 69 283 Cuts at: 2516 3237 4195 442 Size: 721 958 1753 Fragments arranged by size: 1753 958 747 721 416 399 283 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2073 2073 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2762 4166 1239 Size: 1127 396 1404 2579 Fragments arranged by size: 2579 1404 1127 396 Bpu10I CC'TnA_GC Cuts at: 2765 2765 Size: 5506 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2603 2630 3631 4496 4794 2603 Size: 27 1001 865 298 3315 Fragments arranged by size: 3315 1001 865 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4169 882 Size: 617 2670 2219 Fragments arranged by size: 2670 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2131 3297 493 Size: 711 927 1166 2702 Fragments arranged by size: 2702 1166 927 711 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 3313 3695 4330 Size: 53 83 186 936 1101 679 382 635 Cuts at: 4330 275 Size: 1451 Fragments arranged by size: 1451 1101 936 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5281 Fragments arranged by size: 5281 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2420 2528 2792 1186 Size: 1177 57 108 264 3900 Fragments arranged by size: 3900 1177 264 108 57 BseYI C'CCAG_C Cuts at: 4709 5205 4709 Size: 496 5010 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 2462 1163 Size: 573 651 75 4207 Fragments arranged by size: 4207 651 573 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2527 2956 3717 4745 1540 Size: 275 186 526 429 761 1028 2301 Fragments arranged by size: 2301 1028 761 526 429 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2142 2418 2454 2681 3567 3652 4723 2037 Size: 105 276 36 227 886 85 1071 2820 Fragments arranged by size: 2820 1071 886 276 227 105 85 36 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2096 2142 2418 2454 2504 2681 Size: 399 247 59 46 276 36 50 177 Cuts at: 2681 2825 3567 3652 4723 1391 Size: 144 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 247 177 144 85 59 50 46 36 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2861 844 Size: 2017 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3393 1394 Size: 429 1570 3507 Fragments arranged by size: 3507 1570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4001 4175 65 Size: 1183 2753 174 1396 Fragments arranged by size: 2753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4150 4337 1700 Size: 110 2340 187 2869 Fragments arranged by size: 2869 2340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 2443 2463 2465 2529 2531 2533 2603 1925 Size: 518 20 2 64 2 2 70 4828 Fragments arranged by size: 4828 518 70 64 20 2 2 2 BssSI C'ACGA_G Cuts at: 2480 3566 4578 2480 Size: 1086 1012 3408 Fragments arranged by size: 3408 1086 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2485 794 Size: 1691 3815 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2341 2836 3203 3588 3605 5057 5143 5155 Size: 369 495 367 385 17 1452 86 12 Cuts at: 5155 5501 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 495 385 369 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2321 2321 Size: 5506 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4954 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4767 Fragments arranged by size: 4767 683 56 BtsI GCAGTG_nn' Cuts at: 3022 3816 3836 3022 Size: 794 20 4692 Fragments arranged by size: 4692 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2118 2598 Size: 54 1425 50 275 18 198 90 480 Cuts at: 2598 2643 2953 3842 5471 8 Size: 45 310 889 1629 43 Fragments arranged by size: 1629 1425 889 480 310 275 198 90 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2953 1537 Size: 275 1141 4090 Fragments arranged by size: 4090 1141 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 3436 1353 Size: 689 1394 3423 Fragments arranged by size: 3423 1394 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2173 2708 4093 4467 4613 387 Size: 1064 138 584 535 1385 374 146 1280 Fragments arranged by size: 1385 1280 1064 584 535 374 146 138 EcoICRI GAG'CTC Cuts at: 2416 2452 2416 Size: 36 5470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2366 2762 3569 4166 4953 5221 Size: 393 598 136 396 807 597 787 268 Cuts at: 5221 1239 Size: 1524 Fragments arranged by size: 1524 807 787 598 597 396 393 268 136 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2324 2303 Size: 21 5485 EcoO109I rG'GnC_Cy Cuts at: 2113 2317 2713 2113 Size: 204 396 4906 Fragments arranged by size: 4906 396 204 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2776 2776 Size: 5506 FspI TGC'GCA Cuts at: 2555 4012 2555 Size: 1457 4049 HaeII r_GCGC'y Cuts at: 1678 2596 2637 2772 4653 1678 Size: 918 41 135 1881 2531 Fragments arranged by size: 2531 1881 918 135 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2326 2358 4147 Size: 32 135 32 578 32 144 32 1789 Cuts at: 4147 4179 4221 4253 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1789 578 144 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KasI G'GCGC_C Cuts at: 2633 2633 Size: 5506 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 4804 1305 Size: 3315 184 2007 Fragments arranged by size: 3315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 3396 Fragments arranged by size: 3396 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 3464 3823 3982 518 Size: 648 2298 359 159 2042 Fragments arranged by size: 2298 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2151 2440 2922 3599 4747 4992 1606 Size: 545 289 482 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 482 289 245 NarI GG'CG_CC Cuts at: 2634 2634 Size: 5506 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4954 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NspI r_CATG'y Cuts at: 1132 1229 2864 4409 1132 Size: 97 1635 1545 2229 Fragments arranged by size: 2229 1635 1545 97 PasI CC'CwG_GG Cuts at: 1348 2097 1348 Size: 749 4757 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 2596 1108 Size: 1323 165 4018 Fragments arranged by size: 4018 1323 165 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2713 2113 Size: 600 4906 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5506 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PspXI vC'TCGA_Gb Cuts at: 2708 2708 Size: 5506 PstI C_TGCA'G Cuts at: 830 2858 830 Size: 2028 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 SacI G_AGCT'C Cuts at: 2418 2454 2418 Size: 36 5470 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SapI GCTCTTCn'nnn_ Cuts at: 2042 2042 Size: 5506 SbfI CC_TGCA'GG Cuts at: 2858 2858 Size: 5506 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2572 2854 3989 4670 4861 826 Size: 219 1527 282 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 681 282 219 191 SfoI GGC'GCC Cuts at: 2635 2635 Size: 5506 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2833 1872 Size: 961 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 2618 2708 2774 3610 4511 Size: 197 926 666 9 90 66 836 901 Cuts at: 4511 4773 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 836 666 262 197 90 66 9 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 2727 1517 Size: 1210 4296 StyI C'CwwG_G Cuts at: 513 1065 2025 2187 2361 2658 2722 2945 513 Size: 552 960 162 174 297 64 223 3074 Fragments arranged by size: 3074 960 552 297 223 174 162 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2816 3533 3550 3703 3888 4333 325 Size: 1255 1236 717 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1236 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 3752 5464 Size: 274 80 33 51 1443 804 971 1712 Cuts at: 5464 96 Size: 138 Fragments arranged by size: 1712 1443 971 804 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5466 5498 5466 Size: 32 5474 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2128 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 2708 2774 1943 Size: 765 66 4675 Fragments arranged by size: 4675 765 66 XmaI C'CCGG_G Cuts at: 1870 2831 1870 Size: 961 4545 XmnI GAAnn'nnTTC Cuts at: 3635 3635 Size: 5506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3314 276 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI