(Circular) (Six-base) MAPSORT of: pFN21AE5115.seq Check: 4781 from: 1 to: 5023 pFN21AE5115 5023 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2078 2078 Size: 5023 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2833 278 Size: 53 83 186 936 1297 2468 Fragments arranged by size: 2468 1297 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2366 1937 Size: 429 4594 Acc65I G'GTAC_C Cuts at: 2344 2344 Size: 5023 AclI AA'CG_TT Cuts at: 1563 3150 3523 1563 Size: 1587 373 3063 Fragments arranged by size: 3063 1587 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3086 4470 4738 1632 Size: 1454 1384 268 1917 Fragments arranged by size: 1917 1454 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4826 AflIII A'CryG_T Cuts at: 1203 3922 4899 1203 Size: 2719 977 1327 Fragments arranged by size: 2719 1327 977 AgeI A'CCGG_T Cuts at: 3854 3854 Size: 5023 AhdI GACnn_n'nnGTC Cuts at: 3752 3752 Size: 5023 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5023 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5023 AlwNI CAG_nnn'CTG Cuts at: 2766 4338 2766 Size: 1572 3451 ApaI G_GGCC'C Cuts at: 2092 2092 Size: 5023 ApaLI G'TGCA_C Cuts at: 3080 4236 3080 Size: 1156 3867 ApoI r'AATT_y Cuts at: 1493 2135 2332 2558 2710 4819 1493 Size: 642 197 226 152 2109 1697 Fragments arranged by size: 2109 1697 642 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 3577 160 Size: 872 2545 1606 Fragments arranged by size: 2545 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5023 AvaI C'yCGr_G Cuts at: 1870 1943 2217 2348 1870 Size: 73 274 131 4545 Fragments arranged by size: 4545 274 131 73 BamHI G'GATC_C Cuts at: 2353 2720 2353 Size: 367 4656 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 2344 3799 618 Size: 325 217 627 425 132 1455 1842 Fragments arranged by size: 1842 1455 627 425 325 217 132 BanII G_rGCy'C Cuts at: 1391 2092 2317 2342 1391 Size: 701 225 25 4072 Fragments arranged by size: 4072 701 225 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4052 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3214 3248 1903 Size: 34 1277 34 3678 Fragments arranged by size: 3678 1277 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2915 4125 2915 Size: 1210 3813 BclI T'GATC_A Cuts at: 1548 1548 Size: 5023 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3634 136 Size: 107 122 71 631 605 1962 1525 Fragments arranged by size: 1962 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5018 5018 Size: 5023 BlpI GC'TnA_GC Cuts at: 2440 2440 Size: 5023 Bme1580I G_kGCm'C Cuts at: 1790 2092 3084 4240 1790 Size: 302 992 1156 2573 Fragments arranged by size: 2573 1156 992 302 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5023 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2754 3712 442 Size: 416 53 83 399 24 1337 958 1753 Fragments arranged by size: 1753 1337 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5023 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3683 1239 Size: 2444 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1995 3148 4013 4311 1995 Size: 1153 865 298 2707 Fragments arranged by size: 2707 1153 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3686 882 Size: 617 2187 2219 Fragments arranged by size: 2219 2187 617 BsaAI yAC'GTr Cuts at: 493 1204 2814 493 Size: 711 1610 2702 Fragments arranged by size: 2702 1610 711 BsaBI GATnn'nnATC Cuts at: 5017 5017 Size: 5023 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2830 3212 3847 275 Size: 53 83 186 936 1297 382 635 1451 Fragments arranged by size: 1451 1297 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3456 3854 4128 4275 4686 3456 Size: 398 274 147 411 3793 Fragments arranged by size: 3793 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4798 Fragments arranged by size: 4798 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5023 BseYI C'CCAG_C Cuts at: 4226 4722 4226 Size: 496 4527 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2473 3234 4262 1540 Size: 275 186 472 761 1028 2301 Fragments arranged by size: 2301 1028 761 472 275 186 BsiHKAI G_wGCw'C Cuts at: 2317 3084 3169 4240 2317 Size: 767 85 1071 3100 Fragments arranged by size: 3100 1071 767 85 BsmI GAATG_Cn' Cuts at: 2534 2627 2534 Size: 93 4930 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2317 2342 3084 3169 4240 1391 Size: 399 302 225 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 225 85 25 BspEI T'CCGG_A Cuts at: 4686 4686 Size: 5023 BspHI T'CATG_A Cuts at: 2912 4642 2912 Size: 1730 3293 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 2311 2378 844 Size: 1234 233 67 3489 Fragments arranged by size: 3489 1234 233 67 BsrBI CCG'CTC Cuts at: 1394 1823 2910 1394 Size: 429 1087 3507 Fragments arranged by size: 3507 1087 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3518 3692 65 Size: 1183 2270 174 1396 Fragments arranged by size: 2270 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3667 3854 1700 Size: 110 1857 187 2869 Fragments arranged by size: 2869 1857 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5023 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5023 BssSI C'ACGA_G Cuts at: 3083 4095 3083 Size: 1012 4011 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5023 BstBI TT'CG_AA Cuts at: 3827 3827 Size: 5023 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5023 BstYI r'GATC_y Cuts at: 1972 2097 2353 2720 3105 3122 4574 4660 4672 Size: 125 256 367 385 17 1452 86 12 Cuts at: 4672 5018 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 256 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3532 Fragments arranged by size: 3532 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4284 Fragments arranged by size: 4284 683 56 BtsI GCAGTG_nn' Cuts at: 2260 2539 3333 3353 2260 Size: 279 794 20 3930 Fragments arranged by size: 3930 794 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5023 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4988 DraI TTT'AAA Cuts at: 2327 2327 Size: 5023 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4030 809 Size: 507 2714 1802 Fragments arranged by size: 2714 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2470 3359 4988 Size: 54 1425 50 275 18 640 889 1629 Cuts at: 4988 8 Size: 43 Fragments arranged by size: 1629 1425 889 640 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2470 1537 Size: 275 658 4090 Fragments arranged by size: 4090 658 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2953 1353 Size: 1600 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2262 3610 3984 4130 387 Size: 1064 138 673 1348 374 146 1280 Fragments arranged by size: 1348 1280 1064 673 374 146 138 EcoICRI GAG'CTC Cuts at: 2315 2315 Size: 5023 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3086 3683 4470 4738 1239 Size: 393 1454 597 787 268 1524 Fragments arranged by size: 1524 1454 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2088 2088 Size: 5023 EcoRI G'AATT_C Cuts at: 2332 2710 2332 Size: 378 4645 EcoRV GAT'ATC Cuts at: 2146 2146 Size: 5023 FspI TGC'GCA Cuts at: 3529 3529 Size: 5023 HaeII r_GCGC'y Cuts at: 1678 4170 1678 Size: 2492 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2249 2281 2299 Size: 32 135 32 517 32 128 32 18 Cuts at: 2299 2331 3664 3696 3738 3770 1373 Size: 32 1333 32 42 32 2626 Fragments arranged by size: 2626 1333 517 135 128 42 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 2367 2613 669 Size: 988 281 429 246 3079 Fragments arranged by size: 3079 988 429 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5023 HpaI GTT'AAC Cuts at: 2613 2613 Size: 5023 KpnI G_GTAC'C Cuts at: 2348 2348 Size: 5023 MfeI C'AATT_G Cuts at: 2108 2622 2108 Size: 514 4509 MluI A'CGCG_T Cuts at: 4899 4899 Size: 5023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4137 4321 1305 Size: 2832 184 2007 Fragments arranged by size: 2832 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3201 Fragments arranged by size: 3201 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3340 3499 518 Size: 648 1815 359 159 2042 Fragments arranged by size: 2042 1815 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2439 3116 4264 4509 1606 Size: 833 677 1148 245 2120 Fragments arranged by size: 2120 1148 833 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3532 Fragments arranged by size: 3532 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5023 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5023 NotI GC'GGCC_GC Cuts at: 2470 2470 Size: 5023 NspI r_CATG'y Cuts at: 1132 1229 2381 3926 1132 Size: 97 1152 1545 2229 Fragments arranged by size: 2229 1545 1152 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5023 PciI A'CATG_T Cuts at: 3922 3922 Size: 5023 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5023 PmeI GTTT'AAAC Cuts at: 2327 2327 Size: 5023 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2200 2232 3092 3124 4605 4637 2200 Size: 32 860 32 1481 32 2586 Fragments arranged by size: 2586 1481 860 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5023 PsiI TTA'TAA Cuts at: 2593 2593 Size: 5023 PspOMI G'GGCC_C Cuts at: 2088 2088 Size: 5023 PspXI vC'TCGA_Gb Cuts at: 2217 2217 Size: 5023 PstI C_TGCA'G Cuts at: 830 2084 2322 2375 830 Size: 1254 238 53 3478 Fragments arranged by size: 3478 1254 238 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5023 SacI G_AGCT'C Cuts at: 2317 2317 Size: 5023 SalI G'TCGA_C Cuts at: 1936 2365 1936 Size: 429 4594 SbfI CC_TGCA'GG Cuts at: 2375 2375 Size: 5023 ScaI AGT'ACT Cuts at: 3271 4983 3271 Size: 1712 3311 SfcI C'TryA_G Cuts at: 826 1045 2080 2318 2371 3506 4187 4378 826 Size: 219 1035 238 53 1135 681 191 1471 Fragments arranged by size: 1471 1135 1035 681 238 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5023 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5023 SmaI CCC'GGG Cuts at: 1872 2350 1872 Size: 478 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 2217 3127 4028 4290 820 Size: 197 926 67 207 910 901 262 1553 Fragments arranged by size: 1553 926 910 901 262 207 197 67 SnaBI TAC'GTA Cuts at: 493 2814 493 Size: 2321 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5023 SphI G_CATG'C Cuts at: 1132 2381 1132 Size: 1249 3774 SspI AAT'ATT Cuts at: 5 52 2947 5 Size: 47 2895 2081 Fragments arranged by size: 2895 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5023 StyI C'CwwG_G Cuts at: 513 1065 2004 2462 513 Size: 552 939 458 3074 Fragments arranged by size: 3074 939 552 458 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3050 3067 3220 3405 3850 325 Size: 1255 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3269 4981 96 Size: 274 80 33 51 1443 1292 1712 138 Fragments arranged by size: 1712 1443 1292 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4983 5015 4983 Size: 32 4991 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5023 XbaI T'CTAG_A Cuts at: 2359 2359 Size: 5023 XhoI C'TCGA_G Cuts at: 1943 2217 1943 Size: 274 4749 XmaI C'CCGG_G Cuts at: 1870 2348 1870 Size: 478 4545 XmnI GAAnn'nnTTC Cuts at: 3152 3152 Size: 5023 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2831 276 Size: 53 83 186 936 1297 2468 Fragments arranged by size: 2468 1297 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI