################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 190 GACGTC GACGT^C AccI 868 GTAGAC GT^[AC][GT]AC AccIII 790 TCCGGA T^CCGGA AhdI 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 162 GGGCCC GGGCC^C AspEI 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 939 GTATCC GTATCC(6/5) BglI 91 GCCGATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 472 TACGTG [CT]AC^GT[GA] BsaI 259 GAGACC GGTCTC(1/5) BseAI 790 TCCGGA T^CCGGA BsgI 277 GTGCAG GTGCAG(16/14) BsmBI 1204 GAGACG CGTCTC(1/5) BspEI 790 TCCGGA T^CCGGA BssHII 331 GCGCGC G^CGCGC BstEII 549 GGTCACC G^GT[ACGT]ACC BstPI 549 GGTCACC G^GT[ACGT]ACC Eam1105I 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 549 GGTCACC G^GT[ACGT]ACC KspI 825 CCGCGG CCGC^GG MroI 790 TCCGGA T^CCGGA MseI 350 TTAA T^TAA MslI 468 CATTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 885 CCATGG C^CATGG NspI 701 GCATGT [GA]CATG^[CT] PflFI 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 366 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 128 AGGACCT [GA]G^G[AT]CC[CT] PstI 142 CTGCAG CTGCA^G PvuII 145 CAGCTG CAG^CTG SacI 1138 GAGCTC GAGCT^C SacII 825 CCGCGG CCGC^GG ScaI 1121 AGTACT AGT^ACT SmlI 590 CTCAAG C^T[CT][GA]AG StuI 899 AGGCCT AGG^CCT Tru9I 350 TTAA T^TAA Tth111I 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 366 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 839 CCAATTCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 332 CGCG CG^CG 826 CGCG AcyI 190 GACGTC G[GA]^CG[CT]C 461 GGCGTC AfaI 106 GTAC GT^AC 1122 GTAC AvaI 326 CCCGGG C^[CT]CG[GA]G 539 CCCGGG BanII 162 GGGCCC G[GA]GC[CT]^C 1138 GAGCTC BglII 227 AGATCT A^GATCT 380 AGATCT BsaHI 190 GACGTC G[GA]^CG[CT]C 461 GGCGTC BsaWI 320 TCCGGT [AT]^CCGG[AT] 790 TCCGGA BsoBI 326 CCCGGG C^[CT]CG[GA]G 539 CCCGGG BspMI 140 ACCTGC ACCTGC(4/8) 398 ACCTGC BstAPI 66 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 279 GCAGTCGCTGC BstUI 332 CGCG CG^CG 826 CGCG BstXI 508 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 555 CCAGGCCAGTGG HaeII 1154 GGCGCT [GA]GCGC^[CT] 1215 GGCGCT Hin1I 190 GACGTC G[GA]^CG[CT]C 461 GGCGTC MboII 582 GAAGA GAAGA(8/7) 644 TCTTC MspA1I 145 CAGCTG C[AC]G^C[GT]G 825 CCGCGG MvnI 332 CGCG CG^CG 826 CGCG PleI 714 GACTC GAGTC(4/5) 238 GAGTC RsaI 106 GTAC GT^AC 1122 GTAC SmaI 326 CCCGGG CCC^GGG 539 CCCGGG Tsp509I 841 AATT ^AATT 1068 AATT XmaCI 326 CCCGGG C^CCGGG 539 CCCGGG XmaI 326 CCCGGG C^CCGGG 539 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII