(Circular) (Six-base) MAPSORT of: pFN21AE5501.seq Check: 4373 from: 1 to: 5962 pFN21AE5501 5962 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2154 2154 Size: 5962 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2199 3772 278 Size: 53 83 186 936 663 1573 2468 Fragments arranged by size: 2468 1573 936 663 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3305 1937 Size: 937 431 4594 Fragments arranged by size: 4594 937 431 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 4025 5409 5677 1632 Size: 872 1521 1384 268 1917 Fragments arranged by size: 1917 1521 1384 872 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 2284 4691 2284 Size: 2407 3555 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2300 2332 2300 Size: 32 5930 AlwNI CAG_nnn'CTG Cuts at: 2290 2772 2975 3705 5277 2290 Size: 482 203 730 1572 2975 Fragments arranged by size: 2975 1572 730 482 203 ApaI G_GGCC'C Cuts at: 2171 2171 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2544 3287 1870 Size: 73 388 213 743 4545 Fragments arranged by size: 4545 743 388 213 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2171 3147 3281 1391 Size: 780 976 134 4072 Fragments arranged by size: 4072 976 780 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2955 3854 5064 2955 Size: 899 1210 3853 Fragments arranged by size: 3853 1210 899 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 4573 136 Size: 107 122 71 631 605 430 2471 1525 Fragments arranged by size: 2471 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 2232 2385 5957 2232 Size: 153 3572 2237 Fragments arranged by size: 3572 2237 153 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2171 2208 4023 5179 1790 Size: 381 37 1815 1156 2573 Fragments arranged by size: 2573 1815 1156 381 37 BmgBI CAC'GTC Cuts at: 1318 2281 3213 1318 Size: 963 932 4067 Fragments arranged by size: 4067 963 932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2782 3165 3693 Size: 416 53 83 399 24 1365 383 528 Cuts at: 3693 4651 442 Size: 958 1753 Fragments arranged by size: 1753 1365 958 528 416 399 383 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2159 2261 2399 2675 4622 1239 Size: 911 9 102 138 276 1947 2579 Fragments arranged by size: 2579 1947 911 276 138 102 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2580 4087 4952 5250 2580 Size: 1507 865 298 3292 Fragments arranged by size: 3292 1507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4625 882 Size: 617 759 2367 2219 Fragments arranged by size: 2367 2219 759 617 BsaAI yAC'GTr Cuts at: 493 1204 2479 3753 493 Size: 711 1275 1274 2702 Fragments arranged by size: 2702 1275 1274 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 2467 3769 4151 Size: 53 83 186 936 663 271 1302 382 Cuts at: 4151 4786 275 Size: 635 1451 Fragments arranged by size: 1451 1302 936 663 635 382 271 186 83 53 BsaWI w'CCGG_w Cuts at: 2325 2795 4395 4793 5067 5214 5625 2325 Size: 470 1600 398 274 147 411 2662 Fragments arranged by size: 2662 1600 470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 2300 2330 2849 Size: 30 165 30 765 30 102 30 519 Cuts at: 2849 2879 2912 2942 1178 Size: 30 33 30 4198 Fragments arranged by size: 4198 765 519 165 102 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 2396 2927 1186 Size: 1129 81 531 4221 Fragments arranged by size: 4221 1129 531 81 BseYI C'CCAG_C Cuts at: 2213 2931 5165 5661 2213 Size: 718 2234 496 2514 Fragments arranged by size: 2514 2234 718 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 1163 Size: 573 567 4822 Fragments arranged by size: 4822 573 567 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2145 3147 3243 4023 4108 5179 2082 Size: 63 1002 96 780 85 1071 2865 Fragments arranged by size: 2865 1071 1002 780 96 85 63 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 BsmBI CGTCTCn'nnnn_ Cuts at: 3203 3203 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2145 2171 2208 3147 3243 3281 Size: 399 292 63 26 37 939 96 38 Cuts at: 3281 4023 4108 5179 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 939 742 399 292 96 85 63 38 37 26 BspEI T'CCGG_A Cuts at: 2795 5625 2795 Size: 2830 3132 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2412 3317 844 Size: 1310 258 905 3489 Fragments arranged by size: 3489 1310 905 258 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 2336 1925 Size: 411 5551 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 2290 794 Size: 1283 213 4466 Fragments arranged by size: 4466 1283 213 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 2554 1184 Size: 1370 4592 BstXI CCAn_nnnn'nTGG Cuts at: 2520 2567 2520 Size: 47 5915 BstYI r'GATC_y Cuts at: 1972 2232 2385 2439 3292 3659 4044 4061 5513 Size: 260 153 54 853 367 385 17 1452 Cuts at: 5513 5599 5611 5957 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 853 385 367 346 260 153 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2248 2830 2890 513 Size: 552 1183 582 60 3585 Fragments arranged by size: 3585 1183 582 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927 Size: 54 1425 50 275 18 1579 889 1629 Cuts at: 5927 8 Size: 43 Fragments arranged by size: 1629 1579 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2429 4549 4923 5069 387 Size: 1064 138 840 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 840 374 146 138 EcoICRI GAG'CTC Cuts at: 3145 3145 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2159 2261 2399 2504 2675 4025 Size: 393 518 9 102 138 105 171 1350 Cuts at: 4025 4622 5409 5677 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1350 787 597 518 393 268 171 138 105 102 9 EcoO109I rG'GnC_Cy Cuts at: 2134 2167 2168 2134 Size: 33 1 5928 Fragments arranged by size: 5928 33 1 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 3148 2077 Size: 1071 4891 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 3163 3224 5109 1678 Size: 1485 61 1885 2531 Fragments arranged by size: 2531 1885 1485 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2849 2881 4603 Size: 32 135 32 698 32 547 32 1722 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1722 698 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 3920 4279 4438 518 Size: 648 1311 1443 359 159 2042 Fragments arranged by size: 2042 1443 1311 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2832 3378 4055 5203 5448 1606 Size: 546 680 546 677 1148 245 2120 Fragments arranged by size: 2120 1148 680 677 546 546 245 NcoI C'CATG_G Cuts at: 513 1065 2890 513 Size: 552 1825 3585 Fragments arranged by size: 3585 1825 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2710 3320 4865 1132 Size: 97 1481 610 1545 2229 Fragments arranged by size: 2229 1545 1481 610 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 1108 Size: 1269 4693 PfoI T'CCnGG_A Cuts at: 2397 2652 3147 2397 Size: 255 495 5212 Fragments arranged by size: 5212 495 255 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2300 2332 4031 4063 5544 5576 2300 Size: 32 1699 32 1481 32 2686 Fragments arranged by size: 2686 1699 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2134 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2167 2167 Size: 5962 PstI C_TGCA'G Cuts at: 830 2151 3314 830 Size: 1321 1163 3478 Fragments arranged by size: 3478 1321 1163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2152 2152 Size: 5962 SacI G_AGCT'C Cuts at: 3147 3147 Size: 5962 SacII CC_GC'GG Cuts at: 2833 2833 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 3128 4210 5922 3128 Size: 1082 1712 3168 Fragments arranged by size: 3168 1712 1082 SfcI C'TryA_G Cuts at: 826 1045 2091 2147 3109 3310 4445 5126 5317 Size: 219 1046 56 962 201 1135 681 191 Cuts at: 5317 826 Size: 1471 Fragments arranged by size: 1471 1135 1046 962 681 219 201 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2333 2546 3289 1872 Size: 461 213 743 4545 Fragments arranged by size: 4545 743 461 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 2595 4066 4967 5229 820 Size: 197 926 652 1471 901 262 1553 Fragments arranged by size: 1553 1471 926 901 652 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 4573 StyI C'CwwG_G Cuts at: 513 1065 2640 2890 3003 3204 3401 513 Size: 552 1575 250 113 201 197 3074 Fragments arranged by size: 3074 1575 552 250 201 197 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 2712 3989 4006 4159 4344 4789 Size: 1255 993 139 1277 17 153 185 445 Cuts at: 4789 325 Size: 1498 Fragments arranged by size: 1498 1277 1255 993 445 185 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3126 4208 5920 Size: 274 80 33 51 1443 1149 1082 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1443 1149 1082 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2912 2944 5922 5954 2168 Size: 32 712 32 2978 32 2176 Fragments arranged by size: 2978 2176 712 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2722 1316 Size: 1406 4556 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 2851 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2331 2544 3287 1870 Size: 461 213 743 4545 Fragments arranged by size: 4545 743 461 213 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2197 3770 276 Size: 53 83 186 936 663 1573 2468 Fragments arranged by size: 2468 1573 936 663 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI