(Linear) (Six-base) MAPSORT of: KIEE5501.seq Check: 4991 from: 1 to: 1257 KIEE5501.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 149 1257 Size: 149 1108 AatII G_ACGT'C Cuts at: 0 194 1257 Size: 194 1063 AccI GT'mk_AC Cuts at: 0 869 1257 Size: 869 388 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 1257 Size: 499 758 AhdI GACnn_n'nnGTC Cuts at: 0 279 1257 Size: 279 978 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 295 327 1257 Size: 295 32 930 Fragments arranged by size: 930 295 32 AlwNI CAG_nnn'CTG Cuts at: 0 285 767 970 1257 Size: 285 482 203 287 Fragments arranged by size: 482 287 285 203 ApaI G_GGCC'C Cuts at: 0 166 1257 Size: 166 1091 AvaI C'yCGr_G Cuts at: 0 326 539 1257 Size: 326 213 718 Fragments arranged by size: 718 326 213 BanII G_rGCy'C Cuts at: 0 166 1142 1257 Size: 166 976 115 Fragments arranged by size: 976 166 115 BciVI GTATCCnnnnn_n' Cuts at: 0 950 1257 Size: 950 307 BglI GCCn_nnn'nGGC Cuts at: 0 97 1257 Size: 97 1160 BglII A'GATC_T Cuts at: 0 227 380 1257 Size: 227 153 877 Fragments arranged by size: 877 227 153 Bme1580I G_kGCm'C Cuts at: 0 166 203 1257 Size: 166 37 1054 Fragments arranged by size: 1054 166 37 BmgBI CAC'GTC Cuts at: 0 276 1208 1257 Size: 276 932 49 Fragments arranged by size: 932 276 49 BmrI ACTGGGnnnn_n' Cuts at: 0 777 1160 1257 Size: 777 383 97 Fragments arranged by size: 777 383 97 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 154 256 394 670 1257 Size: 145 9 102 138 276 587 Fragments arranged by size: 587 276 145 138 102 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 575 1257 Size: 575 682 BsaI GGTCTCn'nnnn_ Cuts at: 0 253 1257 Size: 253 1004 BsaAI yAC'GTr Cuts at: 0 474 1257 Size: 474 783 BsaHI Gr'CG_yC Cuts at: 0 191 462 1257 Size: 191 271 795 Fragments arranged by size: 795 271 191 BsaWI w'CCGG_w Cuts at: 0 320 790 1257 Size: 320 470 467 Fragments arranged by size: 470 467 320 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 163 193 295 325 844 874 907 937 Size: 163 30 102 30 519 30 33 30 Cuts at: 937 1257 Size: 320 Fragments arranged by size: 519 320 163 102 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 310 391 922 1257 Size: 310 81 531 335 Fragments arranged by size: 531 335 310 81 BseYI C'CCAG_C Cuts at: 0 208 926 1257 Size: 208 718 331 Fragments arranged by size: 718 331 208 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 1257 Size: 298 959 BsiHKAI G_wGCw'C Cuts at: 0 77 140 1142 1238 1257 Size: 77 63 1002 96 19 Fragments arranged by size: 1002 96 77 63 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1198 1257 Size: 1198 59 Bsp1286I G_dGCh'C Cuts at: 0 77 140 166 203 1142 1238 1257 Size: 77 63 26 37 939 96 19 Fragments arranged by size: 939 96 77 63 37 26 19 BspEI T'CCGG_A Cuts at: 0 790 1257 Size: 790 467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 149 407 1257 Size: 149 258 850 Fragments arranged by size: 850 258 149 BssHII G'CGCG_C Cuts at: 0 331 1257 Size: 331 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 72 285 1257 Size: 72 213 972 Fragments arranged by size: 972 213 72 BstEII G'GTnAC_C Cuts at: 0 549 1257 Size: 549 708 BstXI CCAn_nnnn'nTGG Cuts at: 0 515 562 1257 Size: 515 47 695 Fragments arranged by size: 695 515 47 BstYI r'GATC_y Cuts at: 0 227 380 434 1257 Size: 227 153 54 823 Fragments arranged by size: 823 227 153 54 BtgI C'CryG_G Cuts at: 0 243 825 885 1257 Size: 243 582 60 372 Fragments arranged by size: 582 372 243 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 424 1257 Size: 424 833 EcoICRI GAG'CTC Cuts at: 0 1140 1257 Size: 1140 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 154 256 394 499 670 1257 Size: 145 9 102 138 105 171 587 Fragments arranged by size: 587 171 145 138 105 102 9 EcoO109I rG'GnC_Cy Cuts at: 0 129 162 163 1257 Size: 129 33 1 1094 Fragments arranged by size: 1094 129 33 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 72 1143 1257 Size: 72 1071 114 Fragments arranged by size: 1071 114 72 HaeII r_GCGC'y Cuts at: 0 1158 1219 1257 Size: 1158 61 38 Fragments arranged by size: 1158 61 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 265 297 844 876 1257 Size: 265 32 547 32 381 Fragments arranged by size: 547 381 265 32 32 MslI CAynn'nnrTG Cuts at: 0 472 1257 Size: 472 785 MspA1I CmG'CkG Cuts at: 0 147 827 1257 Size: 147 680 430 Fragments arranged by size: 680 430 147 NcoI C'CATG_G Cuts at: 0 885 1257 Size: 885 372 NspI r_CATG'y Cuts at: 0 705 1257 Size: 705 552 PflMI CCAn_nnn'nTGG Cuts at: 0 372 1257 Size: 372 885 PfoI T'CCnGG_A Cuts at: 0 392 647 1142 1257 Size: 392 255 495 115 Fragments arranged by size: 495 392 255 115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 295 327 1257 Size: 295 32 930 Fragments arranged by size: 930 295 32 PpuMI rG'GwC_Cy Cuts at: 0 129 1257 Size: 129 1128 PspOMI G'GGCC_C Cuts at: 0 162 1257 Size: 162 1095 PstI C_TGCA'G Cuts at: 0 146 1257 Size: 146 1111 PvuII CAG'CTG Cuts at: 0 147 1257 Size: 147 1110 SacI G_AGCT'C Cuts at: 0 1142 1257 Size: 1142 115 SacII CC_GC'GG Cuts at: 0 828 1257 Size: 828 429 ScaI AGT'ACT Cuts at: 0 1123 1257 Size: 1123 134 SfcI C'TryA_G Cuts at: 0 86 142 1104 1257 Size: 86 56 962 153 Fragments arranged by size: 962 153 86 56 SmaI CCC'GGG Cuts at: 0 328 541 1257 Size: 328 213 716 Fragments arranged by size: 716 328 213 SmlI C'TyrA_G Cuts at: 0 590 1257 Size: 590 667 StuI AGG'CCT Cuts at: 0 901 1257 Size: 901 356 StyI C'CwwG_G Cuts at: 0 635 885 998 1199 1257 Size: 635 250 113 201 58 Fragments arranged by size: 635 250 201 113 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 568 707 1257 Size: 568 139 550 Fragments arranged by size: 568 550 139 TatI w'GTAC_w Cuts at: 0 1121 1257 Size: 1121 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 163 195 907 939 1257 Size: 163 32 712 32 318 Fragments arranged by size: 712 318 163 32 32 Tth111I GACn'n_nGTC Cuts at: 0 717 1257 Size: 717 540 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 846 1257 Size: 846 411 XmaI C'CCGG_G Cuts at: 0 326 539 1257 Size: 326 213 718 Fragments arranged by size: 718 326 213 ZraI GAC'GTC Cuts at: 0 192 1257 Size: 192 1065 Enzymes that do cut: AarI AatII AccI AcuI AhdI AloI AlwNI ApaI AvaI BanII BciVI BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BssHII BstAPI BstEII BstXI BstYI BtgI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MslI MspA1I NcoI NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII ScaI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI BmtI BplI Bpu10I BsaBI BsiEI BsiWI BsmI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI