################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TseI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccII 21 CGCG CG^CG AfaI 368 GTAC GT^AC AluI 305 AGCT AG^CT Asp700I 99 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 577 GAAGAC GAAGAC(2/6) BpuAI 577 GAAGAC GAAGAC(2/6) BsmFI 263 GGGAC GGGAC(10/14) BspHI 537 TCATGA T^CATGA BsrI 455 ACTGG ACTGG(1/-1) BstBI 401 TTCGAA TT^CGAA BstF5I 240 CATCC GGATG(2/0) BstUI 21 CGCG CG^CG DdeI 165 CTTAG C^T[ACGT]AG DraI 489 TTTAAA TTT^AAA EarI 297 GAAGAG CTCTTC(1/4) FokI 240 CATCC GGATG(9/13) HphI 522 GGTGA GGTGA(8/7) Ksp632I 297 GAAGAG CTCTTC(1/4) MaeIII 40 GTCAC ^GT[ACGT]AC MvnI 21 CGCG CG^CG NspV 401 TTCGAA TT^CGAA PflMI 432 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 30 GACTC GAGTC(4/5) RcaI 537 TCATGA T^CATGA RsaI 368 GTAC GT^AC SfuI 401 TTCGAA TT^CGAA SspI 382 AATATT AAT^ATT TaqI 402 TCGA T^CGA Tsp45I 40 GTCAC ^GT[GC]AC TspRI 371 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 402 TCGA T^CGA Van91I 432 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 99 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 22 GCGG CCGC(-3/-1) 517 GCGG AcsI 353 AAATTC [GA]^AATT[CT] 607 GAATTT ApoI 353 AAATTC [GA]^AATT[CT] 607 GAATTT BstYI 415 AGATCC [GA]^GATC[CT] 441 GGATCT MflI 415 AGATCC [GA]^GATC[CT] 441 GGATCT MslI 205 CATAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 535 CATCATGATG NlaIV 178 GGCTCC GG[ACGT]^[ACGT]CC 263 GGGACC SfaNI 541 GATGC GCATC(5/9) 239 GCATC TfiI 399 GATTC G^A[AT]TC 424 GAATC XhoII 415 AGATCC [GA]^GATC[CT] 441 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AlwI AvaII BpmI BsaJI BsiYI BslI BssKI BstNI Cfr13I DpnII EcoRII HinfI MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI Tru9I Tsp509I